Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin Secretion
Phenolic compounds from natural products are considered effective enhancers of insulin secretion to prevent and treat type 2 diabetes (T2DM). The flowers of <i>Prunus persica </i>(L.) Batsch also contain many phenolic compounds. In this study, the extract of flowers of P. persica (PRPE)...
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doaj-9d0ad8de2b9547129137d8b56c0e859b2021-02-15T00:00:22ZengMDPI AGBiomolecules2218-273X2021-02-011127927910.3390/biom11020279Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin SecretionDahae Lee0Yutong Qi1Ranhee Kim2Jungbin Song3Hocheol Kim4Hyun Young Kim5Dae Sik Jang6Ki Sung Kang7College of Korean Medicine, Gachon University, Seongnam 13120, KoreaDepartment of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, KoreaDepartment of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaPhenolic compounds from natural products are considered effective enhancers of insulin secretion to prevent and treat type 2 diabetes (T2DM). The flowers of <i>Prunus persica </i>(L.) Batsch also contain many phenolic compounds. In this study, the extract of flowers of P. persica (PRPE) exhibited an insulin secretion effect in a glucose-stimulated insulin secretion (GSIS) assay, which led us to isolate and identify the bioactive compound(s) responsible for these effects. Compounds isolated from PRPE were screened for their efficacy in INS-1 rat pancreatic β-cells. Among them, caffeic acid (5), methyl caffeate (6), ferulic acid (7), chlorogenic acid (8), naringenin (11), nicotiflorin (12), and astragalin (13) isolated from PRPE increased GSIS without inducing cytotoxicity. Interestingly, the GSIS effect of methyl caffeate (6) as a phenolic compound was similar to gliclazide, an antidiabetic sulfonylurea drug. Western blot assay showed that methyl caffeate (6) enhanced the related signaling proteins of the activated pancreatic and duodenal homeobox-1 (PDX-1) and peroxisome proliferator-activated receptor-γ (PPAR-γ), but also the phosphorylation of the total insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), and Akt, which influence β-cell function and insulin secretion. This study provides evidence that methyl caffeate (6) isolated from PRPE may aid in the management of T2DM.https://www.mdpi.com/2218-273X/11/2/279Prunus persica (L.) Batschmethyl caffeateinsulinPI3KAKTPPARγ |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dahae Lee Yutong Qi Ranhee Kim Jungbin Song Hocheol Kim Hyun Young Kim Dae Sik Jang Ki Sung Kang |
spellingShingle |
Dahae Lee Yutong Qi Ranhee Kim Jungbin Song Hocheol Kim Hyun Young Kim Dae Sik Jang Ki Sung Kang Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin Secretion Biomolecules Prunus persica (L.) Batsch methyl caffeate insulin PI3K AKT PPARγ |
author_facet |
Dahae Lee Yutong Qi Ranhee Kim Jungbin Song Hocheol Kim Hyun Young Kim Dae Sik Jang Ki Sung Kang |
author_sort |
Dahae Lee |
title |
Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin Secretion |
title_short |
Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin Secretion |
title_full |
Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin Secretion |
title_fullStr |
Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin Secretion |
title_full_unstemmed |
Methyl Caffeate Isolated from the Flowers of <i>Prunus persica</i> (L.) Batsch Enhances Glucose-Stimulated Insulin Secretion |
title_sort |
methyl caffeate isolated from the flowers of <i>prunus persica</i> (l.) batsch enhances glucose-stimulated insulin secretion |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2021-02-01 |
description |
Phenolic compounds from natural products are considered effective enhancers of insulin secretion to prevent and treat type 2 diabetes (T2DM). The flowers of <i>Prunus persica </i>(L.) Batsch also contain many phenolic compounds. In this study, the extract of flowers of P. persica (PRPE) exhibited an insulin secretion effect in a glucose-stimulated insulin secretion (GSIS) assay, which led us to isolate and identify the bioactive compound(s) responsible for these effects. Compounds isolated from PRPE were screened for their efficacy in INS-1 rat pancreatic β-cells. Among them, caffeic acid (5), methyl caffeate (6), ferulic acid (7), chlorogenic acid (8), naringenin (11), nicotiflorin (12), and astragalin (13) isolated from PRPE increased GSIS without inducing cytotoxicity. Interestingly, the GSIS effect of methyl caffeate (6) as a phenolic compound was similar to gliclazide, an antidiabetic sulfonylurea drug. Western blot assay showed that methyl caffeate (6) enhanced the related signaling proteins of the activated pancreatic and duodenal homeobox-1 (PDX-1) and peroxisome proliferator-activated receptor-γ (PPAR-γ), but also the phosphorylation of the total insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), and Akt, which influence β-cell function and insulin secretion. This study provides evidence that methyl caffeate (6) isolated from PRPE may aid in the management of T2DM. |
topic |
Prunus persica (L.) Batsch methyl caffeate insulin PI3K AKT PPARγ |
url |
https://www.mdpi.com/2218-273X/11/2/279 |
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