Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein
The Rift Valley fever virus (RVFV) is an arthropod-borne virus that can not only cause severe disease in domestic animals but also in humans. However, the licensed vaccines or available therapeutics for humans do not exist. Here, we report two Gn-specific neutralizing antibodies (NAbs), isolated fro...
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doaj-9d0ed1fe82ec4897919df538ac482aaf2020-11-25T00:36:54ZengMDPI AGViruses1999-49152020-02-0112325910.3390/v12030259v12030259Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn ProteinMeng Hao0Guanying Zhang1Shengnan Zhang2Zhengshan Chen3Xiangyang Chi4Yunzhu Dong5Pengfei Fan6Yujiao Liu7Yi Chen8Xiaohong Song9Shuling Liu10Changming Yu11Jianmin Li12Xianzhu Xia13Comparative Medicine Center, Peking Union Medical College (PUMC) and Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Beijing 100021, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaCollege of Wildlife and Protected Areas, Northeast Forestry University, Harbin 150040, ChinaTianjin Institue of Envionmental and Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin 300050, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaBeijing Institute of Biotechnology, Beijing 100071, ChinaComparative Medicine Center, Peking Union Medical College (PUMC) and Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Beijing 100021, ChinaThe Rift Valley fever virus (RVFV) is an arthropod-borne virus that can not only cause severe disease in domestic animals but also in humans. However, the licensed vaccines or available therapeutics for humans do not exist. Here, we report two Gn-specific neutralizing antibodies (NAbs), isolated from a rhesus monkey immunized with recombinant human adenoviruses type 4 expressing Rift Valley fever virus Gn and Gc protein (rHAdV4-GnGcopt). The two NAbs were both able to protect host cells from RVFV infection. The interactions between NAbs and Gn were then characterized to demonstrate that these two NAbs might preclude RVFV glycoprotein rearrangement, hindering the exposure of fusion loops in Gc to endosomal membranes after the virus invades the host cell. The target region for the two NAbs is located in the Gn domain III, implying that Gn is a desired target for developing vaccines and neutralizing antibodies against RVFV.https://www.mdpi.com/1999-4915/12/3/259rift valley fever virus (rvfv)neutralizing antibody (nab)gn proteincritical residues |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meng Hao Guanying Zhang Shengnan Zhang Zhengshan Chen Xiangyang Chi Yunzhu Dong Pengfei Fan Yujiao Liu Yi Chen Xiaohong Song Shuling Liu Changming Yu Jianmin Li Xianzhu Xia |
spellingShingle |
Meng Hao Guanying Zhang Shengnan Zhang Zhengshan Chen Xiangyang Chi Yunzhu Dong Pengfei Fan Yujiao Liu Yi Chen Xiaohong Song Shuling Liu Changming Yu Jianmin Li Xianzhu Xia Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein Viruses rift valley fever virus (rvfv) neutralizing antibody (nab) gn protein critical residues |
author_facet |
Meng Hao Guanying Zhang Shengnan Zhang Zhengshan Chen Xiangyang Chi Yunzhu Dong Pengfei Fan Yujiao Liu Yi Chen Xiaohong Song Shuling Liu Changming Yu Jianmin Li Xianzhu Xia |
author_sort |
Meng Hao |
title |
Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein |
title_short |
Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein |
title_full |
Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein |
title_fullStr |
Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein |
title_full_unstemmed |
Characterization of Two Neutralizing Antibodies against Rift Valley Fever Virus Gn Protein |
title_sort |
characterization of two neutralizing antibodies against rift valley fever virus gn protein |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2020-02-01 |
description |
The Rift Valley fever virus (RVFV) is an arthropod-borne virus that can not only cause severe disease in domestic animals but also in humans. However, the licensed vaccines or available therapeutics for humans do not exist. Here, we report two Gn-specific neutralizing antibodies (NAbs), isolated from a rhesus monkey immunized with recombinant human adenoviruses type 4 expressing Rift Valley fever virus Gn and Gc protein (rHAdV4-GnGcopt). The two NAbs were both able to protect host cells from RVFV infection. The interactions between NAbs and Gn were then characterized to demonstrate that these two NAbs might preclude RVFV glycoprotein rearrangement, hindering the exposure of fusion loops in Gc to endosomal membranes after the virus invades the host cell. The target region for the two NAbs is located in the Gn domain III, implying that Gn is a desired target for developing vaccines and neutralizing antibodies against RVFV. |
topic |
rift valley fever virus (rvfv) neutralizing antibody (nab) gn protein critical residues |
url |
https://www.mdpi.com/1999-4915/12/3/259 |
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1725303671020847104 |