Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis
Corynebacterium pseudotuberculosis is the etiological agent of chronic caseous lymphadenitis. The bacterium infects goats and sheep causing great economic loss worldwide annually. The present work aims to evaluate the efficiency of gold nanoparticles (AuNPs) and AuNPs – laser combined therapy as ant...
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doaj-9d1c6259b75048ec911c0b1d206a7bdb2020-11-24T21:54:37ZengTaylor & Francis GroupInternational Journal of Veterinary Science and Medicine2314-45992017-06-0151232910.1016/j.ijvsm.2017.02.003Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosisMarwah M. Mohamed0Shereen A. Fouad1Hisham A. Elshoky2Gina M. Mohammed3Taher A. Salaheldin4Department of Bacterial Diagnostic Products, Veterinary Serum and Vaccine Research Institute, EgyptDepartment of Bacterial Diagnostic Products, Veterinary Serum and Vaccine Research Institute, EgyptNanotechnology and Advanced Material Central Lab, Agriculture Research Center, EgyptCentral Laboratory for Evaluation Veterinary Biologics, Agriculture Research Center, EgyptNanotechnology and Advanced Material Central Lab, Agriculture Research Center, EgyptCorynebacterium pseudotuberculosis is the etiological agent of chronic caseous lymphadenitis. The bacterium infects goats and sheep causing great economic loss worldwide annually. The present work aims to evaluate the efficiency of gold nanoparticles (AuNPs) and AuNPs – laser combined therapy as antibacterial approaches against C. pseudotuberculosis bacteria in vitro. Gold nanoparticles 25 nm were synthesized by co-precipitation method and characterized by different techniques including; Transmission Electron Microscope (TEM), X-ray Diffraction and Dynamic Light Scattering. Three concentrations of AuNPs (50, 100 and 200 μg/mL) were utilized for estimating the bacterial growth rate and the Minimum Inhibitory Concentration (MIC). The mechanism of interaction between AuNPs and bacteria was evaluated by transmission electron microscopic image analysis. Confocal Laser Scanning Microscopic technique was used to study the cytotoxic action of AuNPs and laser against C. psudotuberculosis. Results revealed that MIC of AuNPs and AuNPs – laser combined therapy were 200 μg/mL and 100 μg/mL respectively. TEM image analysis illustrated that gold nanoparticles penetrated the thick wall of C. psudotuberculosis and accumulated as intracellular agglomerates. Laser light enhanced the antimicrobial activity of gold nanoparticles by at least one fold due to its photo thermal combined effect that might be used as an effective antibacterial approach against C. pseudotuberculosis.http://www.sciencedirect.com/science/article/pii/S2314459917300030C. pseudotuberculosisGold nanoparticlesLaserAntibacterial agentCaseous lymphadenitisSheep and goats |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marwah M. Mohamed Shereen A. Fouad Hisham A. Elshoky Gina M. Mohammed Taher A. Salaheldin |
spellingShingle |
Marwah M. Mohamed Shereen A. Fouad Hisham A. Elshoky Gina M. Mohammed Taher A. Salaheldin Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis International Journal of Veterinary Science and Medicine C. pseudotuberculosis Gold nanoparticles Laser Antibacterial agent Caseous lymphadenitis Sheep and goats |
author_facet |
Marwah M. Mohamed Shereen A. Fouad Hisham A. Elshoky Gina M. Mohammed Taher A. Salaheldin |
author_sort |
Marwah M. Mohamed |
title |
Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis |
title_short |
Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis |
title_full |
Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis |
title_fullStr |
Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis |
title_full_unstemmed |
Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis |
title_sort |
antibacterial effect of gold nanoparticles against corynebacterium pseudotuberculosis |
publisher |
Taylor & Francis Group |
series |
International Journal of Veterinary Science and Medicine |
issn |
2314-4599 |
publishDate |
2017-06-01 |
description |
Corynebacterium pseudotuberculosis is the etiological agent of chronic caseous lymphadenitis. The bacterium infects goats and sheep causing great economic loss worldwide annually. The present work aims to evaluate the efficiency of gold nanoparticles (AuNPs) and AuNPs – laser combined therapy as antibacterial approaches against C. pseudotuberculosis bacteria in vitro. Gold nanoparticles 25 nm were synthesized by co-precipitation method and characterized by different techniques including; Transmission Electron Microscope (TEM), X-ray Diffraction and Dynamic Light Scattering. Three concentrations of AuNPs (50, 100 and 200 μg/mL) were utilized for estimating the bacterial growth rate and the Minimum Inhibitory Concentration (MIC). The mechanism of interaction between AuNPs and bacteria was evaluated by transmission electron microscopic image analysis. Confocal Laser Scanning Microscopic technique was used to study the cytotoxic action of AuNPs and laser against C. psudotuberculosis. Results revealed that MIC of AuNPs and AuNPs – laser combined therapy were 200 μg/mL and 100 μg/mL respectively. TEM image analysis illustrated that gold nanoparticles penetrated the thick wall of C. psudotuberculosis and accumulated as intracellular agglomerates. Laser light enhanced the antimicrobial activity of gold nanoparticles by at least one fold due to its photo thermal combined effect that might be used as an effective antibacterial approach against C. pseudotuberculosis. |
topic |
C. pseudotuberculosis Gold nanoparticles Laser Antibacterial agent Caseous lymphadenitis Sheep and goats |
url |
http://www.sciencedirect.com/science/article/pii/S2314459917300030 |
work_keys_str_mv |
AT marwahmmohamed antibacterialeffectofgoldnanoparticlesagainstcorynebacteriumpseudotuberculosis AT shereenafouad antibacterialeffectofgoldnanoparticlesagainstcorynebacteriumpseudotuberculosis AT hishamaelshoky antibacterialeffectofgoldnanoparticlesagainstcorynebacteriumpseudotuberculosis AT ginammohammed antibacterialeffectofgoldnanoparticlesagainstcorynebacteriumpseudotuberculosis AT taherasalaheldin antibacterialeffectofgoldnanoparticlesagainstcorynebacteriumpseudotuberculosis |
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