Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their Mothers
Early childhood caries (ECC), the most frequent disease of oral cavity in preschool children, is the consequence of microbial, genetic, biochemical, socioeconomic, physical, environmental and health-influencing behavioral factors. To investigate the role of the oral microbiome and the impact of host...
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doaj-9d41c3b9fc4245708b5e26ea081ca20f2020-11-25T01:02:25ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882018-10-01810.3389/fcimb.2018.00361413829Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their MothersYuqiao Zheng0Meng Zhang1Jin Li2Yuhong Li3Fei Teng4Han Jiang5Minquan Du6Hubei-Most KLOS&KLOBM, School & Hospital of Stomatology, Wuhan University, Wuhan City, ChinaHubei-Most KLOS&KLOBM, School & Hospital of Stomatology, Wuhan University, Wuhan City, ChinaHubei-Most KLOS&KLOBM, School & Hospital of Stomatology, Wuhan University, Wuhan City, ChinaHubei-Most KLOS&KLOBM, School & Hospital of Stomatology, Wuhan University, Wuhan City, ChinaQingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Shandong, ref-listChinaHubei-Most KLOS&KLOBM, School & Hospital of Stomatology, Wuhan University, Wuhan City, ChinaHubei-Most KLOS&KLOBM, School & Hospital of Stomatology, Wuhan University, Wuhan City, ChinaEarly childhood caries (ECC), the most frequent disease of oral cavity in preschool children, is the consequence of microbial, genetic, biochemical, socioeconomic, physical, environmental and health-influencing behavioral factors. To investigate the role of the oral microbiome and the impact of host and environmental factors in the occurrence and development of ECC, we studied the supragingival plaques of 14 twin pairs and a set of triplets with discordant caries phenotypes and 15 mothers, applying the Human Oral Microbe Identification using Next Generation Sequencing technique (HOMINGS). A total of 2,293,650 reads revealed 11 phyla, 116 genera, and 139 species of micromiome. Comparative analysis between the caries and caries-free group at species level revealed that the relative abundance of Streptococcus mutans, Lactobacillus fermentum, Actinomyces islaelii, Neisseria sica, and Veilonella dispar was much higher in caries group (P < 0.0001). Furthermore, monozygotic twins exhibited a higher degree of similarity than dizygotic twins. Finally, we analyzed the relationship between environmental factors and the oral microbiome, and our results indicat that the frequency of taking sweet food is associated with ECC. We conclude the following. First, the occurrence of Streptococcus mutans, Lactobacillus fermentum, Neisseria sica, and Veilonella dispar is strongly associated with the occurrence of ECC. Second, host genetic factors influence the oral microbiome composition, while environmental and behavioral factors like the frequency of taking sweet foods have an impact on the distribution of caries-related bacteria.https://www.frontiersin.org/article/10.3389/fcimb.2018.00361/fullearly childhood cariessupragingival plaquetwinsoral microbiomemother |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuqiao Zheng Meng Zhang Jin Li Yuhong Li Fei Teng Han Jiang Minquan Du |
spellingShingle |
Yuqiao Zheng Meng Zhang Jin Li Yuhong Li Fei Teng Han Jiang Minquan Du Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their Mothers Frontiers in Cellular and Infection Microbiology early childhood caries supragingival plaque twins oral microbiome mother |
author_facet |
Yuqiao Zheng Meng Zhang Jin Li Yuhong Li Fei Teng Han Jiang Minquan Du |
author_sort |
Yuqiao Zheng |
title |
Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their Mothers |
title_short |
Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their Mothers |
title_full |
Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their Mothers |
title_fullStr |
Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their Mothers |
title_full_unstemmed |
Comparative Analysis of the Microbial Profiles in Supragingival Plaque Samples Obtained From Twins With Discordant Caries Phenotypes and Their Mothers |
title_sort |
comparative analysis of the microbial profiles in supragingival plaque samples obtained from twins with discordant caries phenotypes and their mothers |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2018-10-01 |
description |
Early childhood caries (ECC), the most frequent disease of oral cavity in preschool children, is the consequence of microbial, genetic, biochemical, socioeconomic, physical, environmental and health-influencing behavioral factors. To investigate the role of the oral microbiome and the impact of host and environmental factors in the occurrence and development of ECC, we studied the supragingival plaques of 14 twin pairs and a set of triplets with discordant caries phenotypes and 15 mothers, applying the Human Oral Microbe Identification using Next Generation Sequencing technique (HOMINGS). A total of 2,293,650 reads revealed 11 phyla, 116 genera, and 139 species of micromiome. Comparative analysis between the caries and caries-free group at species level revealed that the relative abundance of Streptococcus mutans, Lactobacillus fermentum, Actinomyces islaelii, Neisseria sica, and Veilonella dispar was much higher in caries group (P < 0.0001). Furthermore, monozygotic twins exhibited a higher degree of similarity than dizygotic twins. Finally, we analyzed the relationship between environmental factors and the oral microbiome, and our results indicat that the frequency of taking sweet food is associated with ECC. We conclude the following. First, the occurrence of Streptococcus mutans, Lactobacillus fermentum, Neisseria sica, and Veilonella dispar is strongly associated with the occurrence of ECC. Second, host genetic factors influence the oral microbiome composition, while environmental and behavioral factors like the frequency of taking sweet foods have an impact on the distribution of caries-related bacteria. |
topic |
early childhood caries supragingival plaque twins oral microbiome mother |
url |
https://www.frontiersin.org/article/10.3389/fcimb.2018.00361/full |
work_keys_str_mv |
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