Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects
We report the isolation of a bacterium from Galleria mellonella larva and its identification using genome sequencing and phylogenomic analysis. This bacterium was named Alcaligenes faecalis strain MOR02. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of th...
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doaj-9d5597825b164d50af7f1a15096cb3c02020-11-24T22:50:47ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/570243570243Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to InsectsRosa Estela Quiroz-Castañeda0Ared Mendoza-Mejía1Verónica Obregón-Barboza2Fernando Martínez-Ocampo3Armando Hernández-Mendoza4Felipe Martínez-Garduño5Gabriel Guillén-Solís6Federico Sánchez-Rodríguez7Guadalupe Peña-Chora8Laura Ortíz-Hernández9Paul Gaytán-Colín10Edgar Dantán-González11Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoFacultad de Ciencias, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoInstituto de Biotecnología, Universidad Nacional Autónoma de México, 62210 Cuernavaca, MOR, MexicoInstituto de Biotecnología, Universidad Nacional Autónoma de México, 62210 Cuernavaca, MOR, MexicoCentro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoInstituto de Biotecnología, Universidad Nacional Autónoma de México, 62210 Cuernavaca, MOR, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, 62210 Cuernavaca, MOR, MexicoWe report the isolation of a bacterium from Galleria mellonella larva and its identification using genome sequencing and phylogenomic analysis. This bacterium was named Alcaligenes faecalis strain MOR02. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of the nematodes Steinernema feltiae, S. carpocapsae, and H. bacteriophora. Using G. mellonella larvae as a model, when the larvae were injected with 24,000 CFU in their hemocoel, more than 96% mortality was achieved after 24 h. Additionally, toxicity assays determined that 1 μg of supernatant extract from A. faecalis MOR02 killed more than 70% G. mellonella larvae 96 h after injection. A correlation of experimental data with sequence genome analyses was also performed. We discovered genes that encode proteins and enzymes that are related to pathogenicity, toxicity, and host/environment interactions that may be responsible for the observed phenotypic characteristics. Our data demonstrates that the bacteria are able to use different strategies to colonize nematodes and kill insects to their own benefit. However, there remains an extensive group of unidentified microorganisms that could be participating in the infection process. Additionally, a nematode-bacterium association could be established probably as a strategy of dispersion and colonization.http://dx.doi.org/10.1155/2015/570243 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rosa Estela Quiroz-Castañeda Ared Mendoza-Mejía Verónica Obregón-Barboza Fernando Martínez-Ocampo Armando Hernández-Mendoza Felipe Martínez-Garduño Gabriel Guillén-Solís Federico Sánchez-Rodríguez Guadalupe Peña-Chora Laura Ortíz-Hernández Paul Gaytán-Colín Edgar Dantán-González |
spellingShingle |
Rosa Estela Quiroz-Castañeda Ared Mendoza-Mejía Verónica Obregón-Barboza Fernando Martínez-Ocampo Armando Hernández-Mendoza Felipe Martínez-Garduño Gabriel Guillén-Solís Federico Sánchez-Rodríguez Guadalupe Peña-Chora Laura Ortíz-Hernández Paul Gaytán-Colín Edgar Dantán-González Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects BioMed Research International |
author_facet |
Rosa Estela Quiroz-Castañeda Ared Mendoza-Mejía Verónica Obregón-Barboza Fernando Martínez-Ocampo Armando Hernández-Mendoza Felipe Martínez-Garduño Gabriel Guillén-Solís Federico Sánchez-Rodríguez Guadalupe Peña-Chora Laura Ortíz-Hernández Paul Gaytán-Colín Edgar Dantán-González |
author_sort |
Rosa Estela Quiroz-Castañeda |
title |
Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects |
title_short |
Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects |
title_full |
Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects |
title_fullStr |
Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects |
title_full_unstemmed |
Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects |
title_sort |
identification of a new alcaligenes faecalis strain mor02 and assessment of its toxicity and pathogenicity to insects |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
We report the isolation of a bacterium from Galleria mellonella larva and its identification using genome sequencing and phylogenomic analysis. This bacterium was named Alcaligenes faecalis strain MOR02. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of the nematodes Steinernema feltiae, S. carpocapsae, and H. bacteriophora. Using G. mellonella larvae as a model, when the larvae were injected with 24,000 CFU in their hemocoel, more than 96% mortality was achieved after 24 h. Additionally, toxicity assays determined that 1 μg of supernatant extract from A. faecalis MOR02 killed more than 70% G. mellonella larvae 96 h after injection. A correlation of experimental data with sequence genome analyses was also performed. We discovered genes that encode proteins and enzymes that are related to pathogenicity, toxicity, and host/environment interactions that may be responsible for the observed phenotypic characteristics. Our data demonstrates that the bacteria are able to use different strategies to colonize nematodes and kill insects to their own benefit. However, there remains an extensive group of unidentified microorganisms that could be participating in the infection process. Additionally, a nematode-bacterium association could be established probably as a strategy of dispersion and colonization. |
url |
http://dx.doi.org/10.1155/2015/570243 |
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