Application of Optical Biosensors in Small-Molecule Screening Activities
The last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise t...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-03-01
|
Series: | Sensors |
Subjects: | |
Online Access: | http://www.mdpi.com/1424-8220/12/4/4311/ |
id |
doaj-9d749b4232e24cb4bad61f2fa065c610 |
---|---|
record_format |
Article |
spelling |
doaj-9d749b4232e24cb4bad61f2fa065c6102020-11-25T00:18:34ZengMDPI AGSensors1424-82202012-03-011244311432310.3390/s120404311Application of Optical Biosensors in Small-Molecule Screening ActivitiesWolfgang KnechtJohan F. CarlssonStefan GeschwindnerThe last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise to match the required throughput without compromising on data quality thus representing a sought-after complement to traditional fluidic systems. This article aims to highlight the application of the two most prominent optical biosensor technologies, namely surface plasmon resonance (SPR) and optical waveguide grating (OWG), in small-molecule screening and will present, review and discuss the advantages and disadvantages of different assay formats on these platforms. A particular focus will be on the specific advantages of the inhibition in solution assay (ISA) format in contrast to traditional direct binding assays (DBA). Furthermore we will discuss different application areas for both fluidic as well as plate-based biosensor systems by considering the individual strength of the platforms.http://www.mdpi.com/1424-8220/12/4/4311/screeningfragmentligandbiosensorsurface plasmon resonanceoptical waveguide gratingdrug discovery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wolfgang Knecht Johan F. Carlsson Stefan Geschwindner |
spellingShingle |
Wolfgang Knecht Johan F. Carlsson Stefan Geschwindner Application of Optical Biosensors in Small-Molecule Screening Activities Sensors screening fragment ligand biosensor surface plasmon resonance optical waveguide grating drug discovery |
author_facet |
Wolfgang Knecht Johan F. Carlsson Stefan Geschwindner |
author_sort |
Wolfgang Knecht |
title |
Application of Optical Biosensors in Small-Molecule Screening Activities |
title_short |
Application of Optical Biosensors in Small-Molecule Screening Activities |
title_full |
Application of Optical Biosensors in Small-Molecule Screening Activities |
title_fullStr |
Application of Optical Biosensors in Small-Molecule Screening Activities |
title_full_unstemmed |
Application of Optical Biosensors in Small-Molecule Screening Activities |
title_sort |
application of optical biosensors in small-molecule screening activities |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2012-03-01 |
description |
The last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise to match the required throughput without compromising on data quality thus representing a sought-after complement to traditional fluidic systems. This article aims to highlight the application of the two most prominent optical biosensor technologies, namely surface plasmon resonance (SPR) and optical waveguide grating (OWG), in small-molecule screening and will present, review and discuss the advantages and disadvantages of different assay formats on these platforms. A particular focus will be on the specific advantages of the inhibition in solution assay (ISA) format in contrast to traditional direct binding assays (DBA). Furthermore we will discuss different application areas for both fluidic as well as plate-based biosensor systems by considering the individual strength of the platforms. |
topic |
screening fragment ligand biosensor surface plasmon resonance optical waveguide grating drug discovery |
url |
http://www.mdpi.com/1424-8220/12/4/4311/ |
work_keys_str_mv |
AT wolfgangknecht applicationofopticalbiosensorsinsmallmoleculescreeningactivities AT johanfcarlsson applicationofopticalbiosensorsinsmallmoleculescreeningactivities AT stefangeschwindner applicationofopticalbiosensorsinsmallmoleculescreeningactivities |
_version_ |
1725375758944174080 |