Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas

The structure of a new phosphoglycolipid from the halophilic Gram-negative bacteria Halomonas elongata ATCC 33173T, Halomonas eurihalina ATCC 49336T, Halomonas almeriensis CECT 7050T, strain Sharm (AM238662), Halomonas halophila DSM 4770T, and Halomonas salina ATCC 49509T was elucidated by NMR and m...

Full description

Bibliographic Details
Main Authors: Assunta Giordano, Filomena M. Vella, Ida Romano, Agata Gambacorta
Format: Article
Language:English
Published: Elsevier 2007-08-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520425179
id doaj-9d7d644c2d1a4b86b0338b015866e8e5
record_format Article
spelling doaj-9d7d644c2d1a4b86b0338b015866e8e52021-04-28T06:07:04ZengElsevierJournal of Lipid Research0022-22752007-08-0148818251831Structural elucidation of a novel phosphoglycolipid isolated from six species of HalomonasAssunta Giordano0Filomena M. Vella1Ida Romano2Agata Gambacorta3Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, 34 80078 Pozzuoli, ItalyIstituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, 34 80078 Pozzuoli, ItalyIstituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, 34 80078 Pozzuoli, ItalyIstituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, 34 80078 Pozzuoli, ItalyThe structure of a new phosphoglycolipid from the halophilic Gram-negative bacteria Halomonas elongata ATCC 33173T, Halomonas eurihalina ATCC 49336T, Halomonas almeriensis CECT 7050T, strain Sharm (AM238662), Halomonas halophila DSM 4770T, and Halomonas salina ATCC 49509T was elucidated by NMR and mass spectroscopy studies. In all of the species examined, the polar lipid composition consisted of 1,2-diacylglycero-3-phosphorylethanolamine, 1,2-diacylglycero-3-phosphoryl-glycerol, bisphosphatidyl glycerol, and the new phosphoglycolipid PGL1. The structure of PGL1 was established to be (2-(α-d-glucopyranosyloxy)-3-hydroxy-propyl)-phosphatidyl diacylglycerol. C16:0;C18:1 and C16:0;C19:cyclopropane are the most abundant acyl chains linked to the phosphatidylglycerol moiety of each isolated PGL1. All of the species presenting the lipid PGL1 belong to Halomonas rRNA group 1, suggesting that the new phosphoglycolipid could be a chemotaxonomic marker of this phylogenetic group.http://www.sciencedirect.com/science/article/pii/S0022227520425179Halomonas elongata ATCC 33173THalomonas eurihalina ATCC 49336THalomonas almeriensis CECT 7050Tstrain Sharm (AM238662)Halomonas halophila DSM 4770THalomonas salina ATCC 49509T
collection DOAJ
language English
format Article
sources DOAJ
author Assunta Giordano
Filomena M. Vella
Ida Romano
Agata Gambacorta
spellingShingle Assunta Giordano
Filomena M. Vella
Ida Romano
Agata Gambacorta
Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas
Journal of Lipid Research
Halomonas elongata ATCC 33173T
Halomonas eurihalina ATCC 49336T
Halomonas almeriensis CECT 7050T
strain Sharm (AM238662)
Halomonas halophila DSM 4770T
Halomonas salina ATCC 49509T
author_facet Assunta Giordano
Filomena M. Vella
Ida Romano
Agata Gambacorta
author_sort Assunta Giordano
title Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas
title_short Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas
title_full Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas
title_fullStr Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas
title_full_unstemmed Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas
title_sort structural elucidation of a novel phosphoglycolipid isolated from six species of halomonas
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 2007-08-01
description The structure of a new phosphoglycolipid from the halophilic Gram-negative bacteria Halomonas elongata ATCC 33173T, Halomonas eurihalina ATCC 49336T, Halomonas almeriensis CECT 7050T, strain Sharm (AM238662), Halomonas halophila DSM 4770T, and Halomonas salina ATCC 49509T was elucidated by NMR and mass spectroscopy studies. In all of the species examined, the polar lipid composition consisted of 1,2-diacylglycero-3-phosphorylethanolamine, 1,2-diacylglycero-3-phosphoryl-glycerol, bisphosphatidyl glycerol, and the new phosphoglycolipid PGL1. The structure of PGL1 was established to be (2-(α-d-glucopyranosyloxy)-3-hydroxy-propyl)-phosphatidyl diacylglycerol. C16:0;C18:1 and C16:0;C19:cyclopropane are the most abundant acyl chains linked to the phosphatidylglycerol moiety of each isolated PGL1. All of the species presenting the lipid PGL1 belong to Halomonas rRNA group 1, suggesting that the new phosphoglycolipid could be a chemotaxonomic marker of this phylogenetic group.
topic Halomonas elongata ATCC 33173T
Halomonas eurihalina ATCC 49336T
Halomonas almeriensis CECT 7050T
strain Sharm (AM238662)
Halomonas halophila DSM 4770T
Halomonas salina ATCC 49509T
url http://www.sciencedirect.com/science/article/pii/S0022227520425179
work_keys_str_mv AT assuntagiordano structuralelucidationofanovelphosphoglycolipidisolatedfromsixspeciesofhalomonas
AT filomenamvella structuralelucidationofanovelphosphoglycolipidisolatedfromsixspeciesofhalomonas
AT idaromano structuralelucidationofanovelphosphoglycolipidisolatedfromsixspeciesofhalomonas
AT agatagambacorta structuralelucidationofanovelphosphoglycolipidisolatedfromsixspeciesofhalomonas
_version_ 1721504146628018176