Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction.
BACKGROUND: MicroRNAs (miRNAs) play key roles in diverse biological and pathological processes, including the regulation of proliferation, apoptosis, angiogenesis and cellular differentiation. Recently, circulating miRNAs have been reported as potential biomarkers for various pathologic conditions....
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doaj-9dc4e66cdb994198a8c30c3dc21caafa2020-11-25T00:11:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5092610.1371/journal.pone.0050926Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction.Guangwen LongFeng WangQuanlu DuanShenglan YangFuqiong ChenWei GongXu YangYan WangChen ChenDao Wen WangBACKGROUND: MicroRNAs (miRNAs) play key roles in diverse biological and pathological processes, including the regulation of proliferation, apoptosis, angiogenesis and cellular differentiation. Recently, circulating miRNAs have been reported as potential biomarkers for various pathologic conditions. This study investigated miR-30a, miR-195 and let-7b as potential of biomarker for acute myocardial infarction (AMI). METHODS AND RESULTS: Plasma samples from 18 patients with AMI and 30 healthy adults were collected. Total RNA was extracted from plasma with TRIzol LS Reagent. MiRNA levels and plasma cardiac troponin I (cTnI) concentrations were measured by quantitative real-time PCR and ELISA assay, respectively. Results showed that circulating miR-30a in AMI patients was highly expressed at 4 h, 8 h and 12 h after onset of AMI, and miR-195 was highly expressed at 8 h and 12 h. However, let-7b was lower in AMI patients than in controls throughout the whole time points. Interestingly, in these patients, circulating miR-30a, miR-195 and let-7b all reached their expression peak at 8 h. By the receiver operating characteristic (ROC) curve analyses, these plasma miRNAs were of significant diagnostic value for AMI. The combined ROC analysis revealed the an AUC value of 0.93 with 94% sensitivity and 90% specificity at 8 h after onset, and an AUC value of 0.92 with 90% sensitivity and 90% specificity at 12 h after onset, in discriminating the AMI patients from healthy controls. CONCLUSIONS: Our results imply that the plasma concentration of miR-30a, miR-195 and let-7b can be potential indicators for AMI.http://europepmc.org/articles/PMC3517576?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guangwen Long Feng Wang Quanlu Duan Shenglan Yang Fuqiong Chen Wei Gong Xu Yang Yan Wang Chen Chen Dao Wen Wang |
spellingShingle |
Guangwen Long Feng Wang Quanlu Duan Shenglan Yang Fuqiong Chen Wei Gong Xu Yang Yan Wang Chen Chen Dao Wen Wang Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction. PLoS ONE |
author_facet |
Guangwen Long Feng Wang Quanlu Duan Shenglan Yang Fuqiong Chen Wei Gong Xu Yang Yan Wang Chen Chen Dao Wen Wang |
author_sort |
Guangwen Long |
title |
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction. |
title_short |
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction. |
title_full |
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction. |
title_fullStr |
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction. |
title_full_unstemmed |
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction. |
title_sort |
circulating mir-30a, mir-195 and let-7b associated with acute myocardial infarction. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
BACKGROUND: MicroRNAs (miRNAs) play key roles in diverse biological and pathological processes, including the regulation of proliferation, apoptosis, angiogenesis and cellular differentiation. Recently, circulating miRNAs have been reported as potential biomarkers for various pathologic conditions. This study investigated miR-30a, miR-195 and let-7b as potential of biomarker for acute myocardial infarction (AMI). METHODS AND RESULTS: Plasma samples from 18 patients with AMI and 30 healthy adults were collected. Total RNA was extracted from plasma with TRIzol LS Reagent. MiRNA levels and plasma cardiac troponin I (cTnI) concentrations were measured by quantitative real-time PCR and ELISA assay, respectively. Results showed that circulating miR-30a in AMI patients was highly expressed at 4 h, 8 h and 12 h after onset of AMI, and miR-195 was highly expressed at 8 h and 12 h. However, let-7b was lower in AMI patients than in controls throughout the whole time points. Interestingly, in these patients, circulating miR-30a, miR-195 and let-7b all reached their expression peak at 8 h. By the receiver operating characteristic (ROC) curve analyses, these plasma miRNAs were of significant diagnostic value for AMI. The combined ROC analysis revealed the an AUC value of 0.93 with 94% sensitivity and 90% specificity at 8 h after onset, and an AUC value of 0.92 with 90% sensitivity and 90% specificity at 12 h after onset, in discriminating the AMI patients from healthy controls. CONCLUSIONS: Our results imply that the plasma concentration of miR-30a, miR-195 and let-7b can be potential indicators for AMI. |
url |
http://europepmc.org/articles/PMC3517576?pdf=render |
work_keys_str_mv |
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