Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM

Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM

The CRISPR/Cas9 system has been widely used for gene editing in zebrafish. However, the required NGG protospacer adjacent motif (PAM) of <i>Streptococcus pyogenes</i> Cas9 (SpCas9) notably restricts the editable range of the zebrafish genome. Recently, Cas9 from <i>S. canis</i&g...

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Bibliographic Details
Main Authors:	Yunxing Liu, Fang Liang, Zijiong Dong, Song Li, Jianmin Ye, Wei Qin
Format:	Article
Language:	English
Published:	MDPI AG 2021-08-01
Series:	Cells
Subjects:	CRISPR/Cas9 ScCas9 gene editing zebrafish NNG PAM
Online Access:	https://www.mdpi.com/2073-4409/10/8/2099

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