Genome Editing in Zebrafish by ScCas9 Recognizing NNG PAM
The CRISPR/Cas9 system has been widely used for gene editing in zebrafish. However, the required NGG protospacer adjacent motif (PAM) of <i>Streptococcus pyogenes</i> Cas9 (SpCas9) notably restricts the editable range of the zebrafish genome. Recently, Cas9 from <i>S. canis</i&g...
Main Authors: | Yunxing Liu, Fang Liang, Zijiong Dong, Song Li, Jianmin Ye, Wei Qin |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-08-01
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Series: | Cells |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4409/10/8/2099 |
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