In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics
Six antimicrobial-producing seaweed-derived Bacillus strains were evaluated in vitro as animal probiotics, in comparison to two Bacillus from an EU-authorized animal probiotic product. Antimicrobial activity was demonstrated on solid media against porcine Salmonella and E. coli. The marine isolates...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2014-04-01
|
Series: | Marine Drugs |
Subjects: | |
Online Access: | http://www.mdpi.com/1660-3397/12/5/2422 |
id |
doaj-9ddb81b32de2488582ed70164b929f0b |
---|---|
record_format |
Article |
spelling |
doaj-9ddb81b32de2488582ed70164b929f0b2020-11-24T21:04:24ZengMDPI AGMarine Drugs1660-33972014-04-011252422244510.3390/md12052422md12052422In Vitro Assessment of Marine Bacillus for Use as Livestock ProbioticsMaria Luz Prieto0Laurie O'Sullivan1Shiau Pin Tan2Peter McLoughlin3Helen Hughes4Montserrat Gutierrez5Jonathan A. Lane6Rita M. Hickey7Peadar G. Lawlor8Gillian E. Gardiner9Eco-Innovation Research Centre, Department of Chemical and Life Sciences, Waterford Institute of Technology, Waterford, IrelandEco-Innovation Research Centre, Department of Chemical and Life Sciences, Waterford Institute of Technology, Waterford, IrelandEco-Innovation Research Centre, Department of Chemical and Life Sciences, Waterford Institute of Technology, Waterford, IrelandEco-Innovation Research Centre, Department of Chemical and Life Sciences, Waterford Institute of Technology, Waterford, IrelandEco-Innovation Research Centre, Department of Chemical and Life Sciences, Waterford Institute of Technology, Waterford, IrelandVeterinary Public Health Regulatory Laboratory, Department of Agriculture, Food and the Marine, Backweston Complex, Celbridge, Co. Kildare, IrelandTeagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, IrelandTeagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, IrelandPig Development Department, Animal and Grassland Research and Innovation Centre, Teagasc, Moorepark, Fermoy, Co. Cork, IrelandEco-Innovation Research Centre, Department of Chemical and Life Sciences, Waterford Institute of Technology, Waterford, IrelandSix antimicrobial-producing seaweed-derived Bacillus strains were evaluated in vitro as animal probiotics, in comparison to two Bacillus from an EU-authorized animal probiotic product. Antimicrobial activity was demonstrated on solid media against porcine Salmonella and E. coli. The marine isolates were most active against the latter, had better activity than the commercial probiotics and Bacillus pumilus WIT 588 also reduced E. coli counts in broth. All of the marine Bacillus tolerated physiological concentrations of bile, with some as tolerant as one of the probiotics. Spore counts for all isolates remained almost constant during incubation in simulated gastric and ileum juices. All of the marine Bacillus grew anaerobically and the spores of all except one isolate germinated under anaerobic conditions. All were sensitive to a panel of antibiotics and none harbored Bacillus enterotoxin genes but all, except B. pumilus WIT 588, showed some degree of β-hemolysis. However, trypan blue dye exclusion and xCELLigence assays demonstrated a lack of toxicity in comparison to two pathogens; in fact, the commercial probiotics appeared more cytotoxic than the majority of the marine Bacillus. Overall, some of the marine-derived Bacillus, in particular B. pumilus WIT 588, demonstrate potential for use as livestock probiotics.http://www.mdpi.com/1660-3397/12/5/2422sporesantimicrobialE. coli xCELLigencepigs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Luz Prieto Laurie O'Sullivan Shiau Pin Tan Peter McLoughlin Helen Hughes Montserrat Gutierrez Jonathan A. Lane Rita M. Hickey Peadar G. Lawlor Gillian E. Gardiner |
spellingShingle |
Maria Luz Prieto Laurie O'Sullivan Shiau Pin Tan Peter McLoughlin Helen Hughes Montserrat Gutierrez Jonathan A. Lane Rita M. Hickey Peadar G. Lawlor Gillian E. Gardiner In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics Marine Drugs spores antimicrobial E. coli xCELLigence pigs |
author_facet |
Maria Luz Prieto Laurie O'Sullivan Shiau Pin Tan Peter McLoughlin Helen Hughes Montserrat Gutierrez Jonathan A. Lane Rita M. Hickey Peadar G. Lawlor Gillian E. Gardiner |
author_sort |
Maria Luz Prieto |
title |
In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics |
title_short |
In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics |
title_full |
In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics |
title_fullStr |
In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics |
title_full_unstemmed |
In Vitro Assessment of Marine Bacillus for Use as Livestock Probiotics |
title_sort |
in vitro assessment of marine bacillus for use as livestock probiotics |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2014-04-01 |
description |
Six antimicrobial-producing seaweed-derived Bacillus strains were evaluated in vitro as animal probiotics, in comparison to two Bacillus from an EU-authorized animal probiotic product. Antimicrobial activity was demonstrated on solid media against porcine Salmonella and E. coli. The marine isolates were most active against the latter, had better activity than the commercial probiotics and Bacillus pumilus WIT 588 also reduced E. coli counts in broth. All of the marine Bacillus tolerated physiological concentrations of bile, with some as tolerant as one of the probiotics. Spore counts for all isolates remained almost constant during incubation in simulated gastric and ileum juices. All of the marine Bacillus grew anaerobically and the spores of all except one isolate germinated under anaerobic conditions. All were sensitive to a panel of antibiotics and none harbored Bacillus enterotoxin genes but all, except B. pumilus WIT 588, showed some degree of β-hemolysis. However, trypan blue dye exclusion and xCELLigence assays demonstrated a lack of toxicity in comparison to two pathogens; in fact, the commercial probiotics appeared more cytotoxic than the majority of the marine Bacillus. Overall, some of the marine-derived Bacillus, in particular B. pumilus WIT 588, demonstrate potential for use as livestock probiotics. |
topic |
spores antimicrobial E. coli xCELLigence pigs |
url |
http://www.mdpi.com/1660-3397/12/5/2422 |
work_keys_str_mv |
AT marialuzprieto invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT laurieosullivan invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT shiaupintan invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT petermcloughlin invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT helenhughes invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT montserratgutierrez invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT jonathanalane invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT ritamhickey invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT peadarglawlor invitroassessmentofmarinebacillusforuseaslivestockprobiotics AT gillianegardiner invitroassessmentofmarinebacillusforuseaslivestockprobiotics |
_version_ |
1716771225701711872 |