Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment
Exposure to phthalates, endocrine-disrupting chemicals commonly used as plasticizers and in consumer products, has been associated with infertility and premature ovarian failure. Our objective was to investigate whether urinary phthalate metabolite concentrations were associated with pre-ovulatory f...
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Elsevier
2021-12-01
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Series: | Environment International |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412021004347 |
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doaj-9ddc8765b72b47a48e5cbbb053adebba |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Caitlin R. Sacha Irene Souter Paige L. Williams Jorge E. Chavarro Jennifer Ford Shruthi Mahalingaiah Patricia K. Donahoe Russ Hauser David Pépin Lidia Mínguez-Alarcón |
spellingShingle |
Caitlin R. Sacha Irene Souter Paige L. Williams Jorge E. Chavarro Jennifer Ford Shruthi Mahalingaiah Patricia K. Donahoe Russ Hauser David Pépin Lidia Mínguez-Alarcón Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment Environment International IVF Follicular fluid AMH Phthalates |
author_facet |
Caitlin R. Sacha Irene Souter Paige L. Williams Jorge E. Chavarro Jennifer Ford Shruthi Mahalingaiah Patricia K. Donahoe Russ Hauser David Pépin Lidia Mínguez-Alarcón |
author_sort |
Caitlin R. Sacha |
title |
Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment |
title_short |
Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment |
title_full |
Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment |
title_fullStr |
Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment |
title_full_unstemmed |
Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment |
title_sort |
urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatment |
publisher |
Elsevier |
series |
Environment International |
issn |
0160-4120 |
publishDate |
2021-12-01 |
description |
Exposure to phthalates, endocrine-disrupting chemicals commonly used as plasticizers and in consumer products, has been associated with infertility and premature ovarian failure. Our objective was to investigate whether urinary phthalate metabolite concentrations were associated with pre-ovulatory follicular fluid (FF) anti-müllerian hormone (AMH) concentrations in women undergoing fertility treatment. This cross-sectional analysis included 138 women with urinary phthalate data available in the Environment and Reproductive Health (EARTH) Study (2010–2016) in whom FF AMH concentrations were quantified using a sandwich enzyme-linked immunosorbent assay (ELISA). We also quantified 8 phthalate metabolite concentrations using tandem mass spectrometry in 1–2 urine samples per cycle (total 331 urines) and calculated the cycle-specific geometric mean for each metabolite. We applied cluster-weighted generalized estimating equation models (CWGEE) to evaluate the associations of tertiles of urinary phthalate metabolite concentrations with log-transformed FF AMH concentrations adjusting for potential confounders. Study participants had median age of 34.0 years (IQR 32.0, 37.0), 83% were white, and median BMI of 23.1 kg/m2 (IQR 21.2, 26.1). The following stimulation protocols were used: luteal phase agonist (70%), antagonist (14%), or flare (16%). Urinary concentrations of select phthalate metabolites were negatively associated with FF AMH. For example, women whose urinary mEOHP was in the lowest tertile (range 0.30–4.04 ng/ml) had an adjusted mean FF AMH of 0.72 ng/mL (95% CI = 0.36, 1.44), compared to women in the highest tertile (range 9.90–235), who had an adjusted mean of 0.24 ng/mL (95% CI = 0.12–0.48, p < 0.05). The negative association between urinary concentrations of certain phthalate metabolites with FF AMH concentrations may have implications for antral follicle recruitment and fertility treatment outcomes. |
topic |
IVF Follicular fluid AMH Phthalates |
url |
http://www.sciencedirect.com/science/article/pii/S0160412021004347 |
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doaj-9ddc8765b72b47a48e5cbbb053adebba2021-10-01T04:46:20ZengElsevierEnvironment International0160-41202021-12-01157106809Urinary phthalate metabolite concentrations are negatively associated with follicular fluid anti-müllerian hormone concentrations in women undergoing fertility treatmentCaitlin R. Sacha0Irene Souter1Paige L. Williams2Jorge E. Chavarro3Jennifer Ford4Shruthi Mahalingaiah5Patricia K. Donahoe6Russ Hauser7David Pépin8Lidia Mínguez-Alarcón9Massachusetts General Hospital Fertility Center, Boston, MA, United States; Massachusetts General Hospital Pediatric Surgical Research Laboratories, Boston, MA, United States; Harvard Medical School, Boston, MA, United States; Corresponding author at: Massachusetts General Hospital Fertility Center and Pediatric Surgery Laboratories, 32 Fruit Street - Yawkey 10A, Boston, MA 02114, United States.Massachusetts General Hospital Fertility Center, Boston, MA, United States; Harvard Medical School, Boston, MA, United StatesDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United StatesDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United States; Department of Nutrition and Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, United States; Channing Division of Network Medicine, Harvard Medical School & Brigham and Women’s Hospital, United StatesDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United StatesMassachusetts General Hospital Fertility Center, Boston, MA, United States; Harvard Medical School, Boston, MA, United States; Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United StatesMassachusetts General Hospital Pediatric Surgical Research Laboratories, Boston, MA, United States; Harvard Medical School, Boston, MA, United StatesDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United StatesMassachusetts General Hospital Pediatric Surgical Research Laboratories, Boston, MA, United States; Harvard Medical School, Boston, MA, United StatesDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United States; Channing Division of Network Medicine, Harvard Medical School & Brigham and Women’s Hospital, United StatesExposure to phthalates, endocrine-disrupting chemicals commonly used as plasticizers and in consumer products, has been associated with infertility and premature ovarian failure. Our objective was to investigate whether urinary phthalate metabolite concentrations were associated with pre-ovulatory follicular fluid (FF) anti-müllerian hormone (AMH) concentrations in women undergoing fertility treatment. This cross-sectional analysis included 138 women with urinary phthalate data available in the Environment and Reproductive Health (EARTH) Study (2010–2016) in whom FF AMH concentrations were quantified using a sandwich enzyme-linked immunosorbent assay (ELISA). We also quantified 8 phthalate metabolite concentrations using tandem mass spectrometry in 1–2 urine samples per cycle (total 331 urines) and calculated the cycle-specific geometric mean for each metabolite. We applied cluster-weighted generalized estimating equation models (CWGEE) to evaluate the associations of tertiles of urinary phthalate metabolite concentrations with log-transformed FF AMH concentrations adjusting for potential confounders. Study participants had median age of 34.0 years (IQR 32.0, 37.0), 83% were white, and median BMI of 23.1 kg/m2 (IQR 21.2, 26.1). The following stimulation protocols were used: luteal phase agonist (70%), antagonist (14%), or flare (16%). Urinary concentrations of select phthalate metabolites were negatively associated with FF AMH. For example, women whose urinary mEOHP was in the lowest tertile (range 0.30–4.04 ng/ml) had an adjusted mean FF AMH of 0.72 ng/mL (95% CI = 0.36, 1.44), compared to women in the highest tertile (range 9.90–235), who had an adjusted mean of 0.24 ng/mL (95% CI = 0.12–0.48, p < 0.05). The negative association between urinary concentrations of certain phthalate metabolites with FF AMH concentrations may have implications for antral follicle recruitment and fertility treatment outcomes.http://www.sciencedirect.com/science/article/pii/S0160412021004347IVFFollicular fluidAMHPhthalates |