From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi
Agriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium refe...
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doaj-9df5dcc409d44c2385b734abc48956d82021-09-26T00:43:59ZengMDPI AGMicroorganisms2076-26072021-09-0191924192410.3390/microorganisms9091924From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic FungiPierre Joly0Alexandra Calteau1Aurélie Wauquier2Rémi Dumas3Mylène Beuvin4David Vallenet5Julien Crovadore6Bastien Cochard7François Lefort8Jean-Yves Berthon9Greentech, Biopôle Clermont-Limagne, 63360 Saint Beauzire, FranceLABGeM, Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Université d’Évry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057 Evry, FranceGreentech, Biopôle Clermont-Limagne, 63360 Saint Beauzire, FranceGreentech, Biopôle Clermont-Limagne, 63360 Saint Beauzire, FranceLABGeM, Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Université d’Évry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057 Evry, FranceLABGeM, Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Université d’Évry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057 Evry, FrancePlants and Pathogens Group, Research Institute Land Nature and Environment, Geneva School of Engineering, Architecture and Landscape (HEPIA), HES-SO University of Applied Sciences and Arts Western Switzerland, 1254 Jussy, SwitzerlandPlants and Pathogens Group, Research Institute Land Nature and Environment, Geneva School of Engineering, Architecture and Landscape (HEPIA), HES-SO University of Applied Sciences and Arts Western Switzerland, 1254 Jussy, SwitzerlandPlants and Pathogens Group, Research Institute Land Nature and Environment, Geneva School of Engineering, Architecture and Landscape (HEPIA), HES-SO University of Applied Sciences and Arts Western Switzerland, 1254 Jussy, SwitzerlandGreentech, Biopôle Clermont-Limagne, 63360 Saint Beauzire, FranceAgriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium referred as strain B25 and belonging to the species <i>Bacillus velezensis</i> (synonym <i>B. amyloliquefaciens subsp. plantarum</i> or <i>B. methylotrophicus</i>), a promising plant growth promoting rhizobacterium (PGPR) and an inhibitor of pathogenic fungi inducing crops diseases. B25 strain activities were investigated. Its genes are well preserved, with their majority being common with other <i>Bacillus spp</i>. strains and responsible for the biosynthesis of secondary metabolites known to be involved in biocontrol and plant growth-promoting activities. No antibiotic resistance genes were found in the B25 strain plasmid. In vitro and in planta tests were conducted to confirm these PGPR and biocontrol properties, showing its efficiency against 13 different pathogenic fungi through antibiosis mechanism. B25 strain also showed good capacities to quickly colonize its environment, to solubilize phosphorus and to produce siderophores and little amounts of auxin-type phytohormones (around 13,051 µg/mL after 32 h). All these findings combined to the fact that B25 demonstrated good properties for industrialization of the production and an environmental-friendly profile, led to its commercialization under market authorization since 2018 in several biostimulant preparations and opened its potential use as a biocontrol agent.https://www.mdpi.com/2076-2607/9/9/1924<i>Bacillus velezensis</i>plant growth-promoting rhizobacteriumbiocontrol agentmicrobial ecotoxicology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pierre Joly Alexandra Calteau Aurélie Wauquier Rémi Dumas Mylène Beuvin David Vallenet Julien Crovadore Bastien Cochard François Lefort Jean-Yves Berthon |
spellingShingle |
Pierre Joly Alexandra Calteau Aurélie Wauquier Rémi Dumas Mylène Beuvin David Vallenet Julien Crovadore Bastien Cochard François Lefort Jean-Yves Berthon From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi Microorganisms <i>Bacillus velezensis</i> plant growth-promoting rhizobacterium biocontrol agent microbial ecotoxicology |
author_facet |
Pierre Joly Alexandra Calteau Aurélie Wauquier Rémi Dumas Mylène Beuvin David Vallenet Julien Crovadore Bastien Cochard François Lefort Jean-Yves Berthon |
author_sort |
Pierre Joly |
title |
From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi |
title_short |
From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi |
title_full |
From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi |
title_fullStr |
From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi |
title_full_unstemmed |
From Strain Characterization to Field Authorization: Highlights on <i>Bacillus velezensis</i> Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi |
title_sort |
from strain characterization to field authorization: highlights on <i>bacillus velezensis</i> strain b25 beneficial properties for plants and its activities on phytopathogenic fungi |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-09-01 |
description |
Agriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium referred as strain B25 and belonging to the species <i>Bacillus velezensis</i> (synonym <i>B. amyloliquefaciens subsp. plantarum</i> or <i>B. methylotrophicus</i>), a promising plant growth promoting rhizobacterium (PGPR) and an inhibitor of pathogenic fungi inducing crops diseases. B25 strain activities were investigated. Its genes are well preserved, with their majority being common with other <i>Bacillus spp</i>. strains and responsible for the biosynthesis of secondary metabolites known to be involved in biocontrol and plant growth-promoting activities. No antibiotic resistance genes were found in the B25 strain plasmid. In vitro and in planta tests were conducted to confirm these PGPR and biocontrol properties, showing its efficiency against 13 different pathogenic fungi through antibiosis mechanism. B25 strain also showed good capacities to quickly colonize its environment, to solubilize phosphorus and to produce siderophores and little amounts of auxin-type phytohormones (around 13,051 µg/mL after 32 h). All these findings combined to the fact that B25 demonstrated good properties for industrialization of the production and an environmental-friendly profile, led to its commercialization under market authorization since 2018 in several biostimulant preparations and opened its potential use as a biocontrol agent. |
topic |
<i>Bacillus velezensis</i> plant growth-promoting rhizobacterium biocontrol agent microbial ecotoxicology |
url |
https://www.mdpi.com/2076-2607/9/9/1924 |
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