Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon
Background: Electrophoresis of serum proteins is an orientation examination routinely used in clinical practice. For a few years, agarose gel electrophoresis has tended to be replaced with capillary electrophoresis owing to an increase in the accuracy of results. However, this technique is uncommo...
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Hamadan University of Medical Sciences
2018-12-01
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doaj-9dfcc7df5430402b8bafe8053063ea012021-08-02T21:04:37ZengHamadan University of Medical SciencesAvicenna Journal of Medical Biochemistry2345-41132018-12-0162505510.15171/ajmb.2018.11ajmb-2142Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, CameroonJudith Gwladys Ekwe Priso0Jean Pierre Nda Mefo’o1Cécile Okalla Ebongue2Eveline Ngouadjeu Dongho Tsakeu3Catherine Akono Ndi4Gérard Beyiha5Marie Solange Doualla6Dieudonné Adiogo7Clinical Biology Laboratory, Douala General Hospital, CameroonClinical Biology Laboratory, Douala General Hospital, CameroonClinical Biology Laboratory, Douala General Hospital, CameroonClinical Biology Laboratory, Douala General Hospital, CameroonClinical Biology Laboratory, Douala General Hospital, CameroonDepartment of Clinical Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala, CameroonDepartment of Clinical Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala, CameroonDepartment of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, Douala, CameroonBackground: Electrophoresis of serum proteins is an orientation examination routinely used in clinical practice. For a few years, agarose gel electrophoresis has tended to be replaced with capillary electrophoresis owing to an increase in the accuracy of results. However, this technique is uncommon and is not widely used in Cameroon. Objectives: The research aimed at studying the electrophoretic profile of serum proteins using capillary technique among patients attending the Douala General Hospital, Cameroon. Methods: Capillary electrophoresis was used to carry out tests on blood samples from any inpatients and outpatients and fasting for 8-12 hours. Capillary electrophoresis of serum samples was used for the separation of proteins into six fractions and the total protidemia of each serum samples was determined using the Biuret method. Results were interpreted by observing the shape of curves and quantitative variations in each fraction of the different serum proteins. Results: A total of 311 patients participated in the study. The sampled population aged 50±18 years on average and consisted of 55.3% men and 44.7% women. All capillary electrophoresis profiles presented six protein fractions, namely, albumin, alpha (1 and 2), beta (1 and 2) and gamma globulins. Pathological disorders were diagnosed in 290 patients and 21 patients had normal results. Inflammatory syndromes accounted for 63.34% and monoclonal gammopathies for 10.29% the main pathological disorder identified. Conclusion: Capillary electrophoresis provides a more precise identification of biological syndromes and clear distinction of the six fractions of each protein. Monoclonal profiles and inflammatory syndromes were well detected. A prevalence of 10.29% was determined for gammopathies.http://ajmb.umsha.ac.ir/PDF/ajmb-2142Electrophoresis profilecapillary techniqueclinical utilityDouala |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Judith Gwladys Ekwe Priso Jean Pierre Nda Mefo’o Cécile Okalla Ebongue Eveline Ngouadjeu Dongho Tsakeu Catherine Akono Ndi Gérard Beyiha Marie Solange Doualla Dieudonné Adiogo |
spellingShingle |
Judith Gwladys Ekwe Priso Jean Pierre Nda Mefo’o Cécile Okalla Ebongue Eveline Ngouadjeu Dongho Tsakeu Catherine Akono Ndi Gérard Beyiha Marie Solange Doualla Dieudonné Adiogo Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon Avicenna Journal of Medical Biochemistry Electrophoresis profile capillary technique clinical utility Douala |
author_facet |
Judith Gwladys Ekwe Priso Jean Pierre Nda Mefo’o Cécile Okalla Ebongue Eveline Ngouadjeu Dongho Tsakeu Catherine Akono Ndi Gérard Beyiha Marie Solange Doualla Dieudonné Adiogo |
author_sort |
Judith Gwladys Ekwe Priso |
title |
Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon |
title_short |
Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon |
title_full |
Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon |
title_fullStr |
Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon |
title_full_unstemmed |
Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon |
title_sort |
electrophoretic profile of serum proteins using capillary technique in patients attending the douala general hospital, cameroon |
publisher |
Hamadan University of Medical Sciences |
series |
Avicenna Journal of Medical Biochemistry |
issn |
2345-4113 |
publishDate |
2018-12-01 |
description |
Background: Electrophoresis of serum proteins is an orientation examination routinely used in clinical practice. For a few years, agarose gel electrophoresis has tended to be replaced with capillary electrophoresis owing to an increase in the accuracy of results. However, this technique is uncommon and is not widely used in Cameroon. Objectives: The research aimed at studying the electrophoretic profile of serum proteins using capillary technique among patients attending the Douala General Hospital, Cameroon. Methods: Capillary electrophoresis was used to carry out tests on blood samples from any inpatients and outpatients and fasting for 8-12 hours. Capillary electrophoresis of serum samples was used for the separation of proteins into six fractions and the total protidemia of each serum samples was determined using the Biuret method. Results were interpreted by observing the shape of curves and quantitative variations in each fraction of the different serum proteins. Results: A total of 311 patients participated in the study. The sampled population aged 50±18 years on average and consisted of 55.3% men and 44.7% women. All capillary electrophoresis profiles presented six protein fractions, namely, albumin, alpha (1 and 2), beta (1 and 2) and gamma globulins. Pathological disorders were diagnosed in 290 patients and 21 patients had normal results. Inflammatory syndromes accounted for 63.34% and monoclonal gammopathies for 10.29% the main pathological disorder identified. Conclusion: Capillary electrophoresis provides a more precise identification of biological syndromes and clear distinction of the six fractions of each protein. Monoclonal profiles and inflammatory syndromes were well detected. A prevalence of 10.29% was determined for gammopathies. |
topic |
Electrophoresis profile capillary technique clinical utility Douala |
url |
http://ajmb.umsha.ac.ir/PDF/ajmb-2142 |
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