Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens
The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-07-01
|
Series: | Animals |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2615/11/7/2150 |
id |
doaj-9e05540144d14b88abec75056b3cdbb3 |
---|---|
record_format |
Article |
spelling |
doaj-9e05540144d14b88abec75056b3cdbb32021-07-23T13:27:55ZengMDPI AGAnimals2076-26152021-07-01112150215010.3390/ani11072150Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral PathogensAlexandra Knox0Travis Beddoe1Department of Animal, Plant and Soil Sciences and Centre for AgriBioscience La Trobe University, Bundoora, VIC 2082, AustraliaDepartment of Animal, Plant and Soil Sciences and Centre for AgriBioscience La Trobe University, Bundoora, VIC 2082, AustraliaThe global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and subsequent significant economic losses. Additionally, horses serve as a host for several zoonotic diseases that could cause significant human health problems. The ability to rapidly diagnose equine viral diseases early could lead to better management, treatment, and biosecurity strategies. Current serological and molecular methods cannot be field-deployable and are not suitable for resource-poor laboratories due to the requirement of expensive equipment and trained personnel. Recently, isothermal nucleic acid amplification technologies, such as loop-mediated isothermal amplification (LAMP) and insulated isothermal polymerase chain reaction (iiPCR), have been developed to be utilized in-field, and provide rapid results within an hour. We will review current isothermal diagnostic techniques available to diagnose equine viruses of biosecurity and zoonotic concern and provide insight into their potential for in-field deployment.https://www.mdpi.com/2076-2615/11/7/2150equinevirusesloop-mediated isothermal amplificationinsulated isothermal polymerase chain reactionfield-deployablepoint-of-care testing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexandra Knox Travis Beddoe |
spellingShingle |
Alexandra Knox Travis Beddoe Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens Animals equine viruses loop-mediated isothermal amplification insulated isothermal polymerase chain reaction field-deployable point-of-care testing |
author_facet |
Alexandra Knox Travis Beddoe |
author_sort |
Alexandra Knox |
title |
Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens |
title_short |
Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens |
title_full |
Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens |
title_fullStr |
Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens |
title_full_unstemmed |
Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens |
title_sort |
isothermal nucleic acid amplification technologies for the detection of equine viral pathogens |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-07-01 |
description |
The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and subsequent significant economic losses. Additionally, horses serve as a host for several zoonotic diseases that could cause significant human health problems. The ability to rapidly diagnose equine viral diseases early could lead to better management, treatment, and biosecurity strategies. Current serological and molecular methods cannot be field-deployable and are not suitable for resource-poor laboratories due to the requirement of expensive equipment and trained personnel. Recently, isothermal nucleic acid amplification technologies, such as loop-mediated isothermal amplification (LAMP) and insulated isothermal polymerase chain reaction (iiPCR), have been developed to be utilized in-field, and provide rapid results within an hour. We will review current isothermal diagnostic techniques available to diagnose equine viruses of biosecurity and zoonotic concern and provide insight into their potential for in-field deployment. |
topic |
equine viruses loop-mediated isothermal amplification insulated isothermal polymerase chain reaction field-deployable point-of-care testing |
url |
https://www.mdpi.com/2076-2615/11/7/2150 |
work_keys_str_mv |
AT alexandraknox isothermalnucleicacidamplificationtechnologiesforthedetectionofequineviralpathogens AT travisbeddoe isothermalnucleicacidamplificationtechnologiesforthedetectionofequineviralpathogens |
_version_ |
1721289816972197888 |