Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells.
Blast-Induced Traumatic Brain Injury (bTBI) describes a spectrum of injuries caused by an explosive force that results in changes in brain function. The mechanism responsible for primary bTBI following a blast shockwave remains unknown. We have developed a pneumatic device that delivers shockwaves,...
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doaj-9e05898d85774f1aa058f845dc3bce252020-11-25T02:15:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3942110.1371/journal.pone.0039421Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells.Rea RavinPaul S BlankAlex SteinkampShay M RappaportNitay RavinLudmila BezrukovHugo Guerrero-CazaresAlfredo Quinones-HinojosaSergey M BezrukovJoshua ZimmerbergBlast-Induced Traumatic Brain Injury (bTBI) describes a spectrum of injuries caused by an explosive force that results in changes in brain function. The mechanism responsible for primary bTBI following a blast shockwave remains unknown. We have developed a pneumatic device that delivers shockwaves, similar to those known to induce bTBI, within a chamber optimal for fluorescence microscopy. Abrupt changes in pressure can be created with and without the presence of shear forces at the surface of cells. In primary cultures of human central nervous system cells, the cellular calcium response to shockwaves alone was negligible. Even when the applied pressure reached 15 atm, there was no damage or excitation, unless concomitant shear forces, peaking between 0.3 to 0.7 Pa, were present at the cell surface. The probability of cellular injury in response to a shockwave was low and cell survival was unaffected 20 hours after shockwave exposure.http://europepmc.org/articles/PMC3387147?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rea Ravin Paul S Blank Alex Steinkamp Shay M Rappaport Nitay Ravin Ludmila Bezrukov Hugo Guerrero-Cazares Alfredo Quinones-Hinojosa Sergey M Bezrukov Joshua Zimmerberg |
spellingShingle |
Rea Ravin Paul S Blank Alex Steinkamp Shay M Rappaport Nitay Ravin Ludmila Bezrukov Hugo Guerrero-Cazares Alfredo Quinones-Hinojosa Sergey M Bezrukov Joshua Zimmerberg Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells. PLoS ONE |
author_facet |
Rea Ravin Paul S Blank Alex Steinkamp Shay M Rappaport Nitay Ravin Ludmila Bezrukov Hugo Guerrero-Cazares Alfredo Quinones-Hinojosa Sergey M Bezrukov Joshua Zimmerberg |
author_sort |
Rea Ravin |
title |
Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells. |
title_short |
Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells. |
title_full |
Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells. |
title_fullStr |
Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells. |
title_full_unstemmed |
Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells. |
title_sort |
shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Blast-Induced Traumatic Brain Injury (bTBI) describes a spectrum of injuries caused by an explosive force that results in changes in brain function. The mechanism responsible for primary bTBI following a blast shockwave remains unknown. We have developed a pneumatic device that delivers shockwaves, similar to those known to induce bTBI, within a chamber optimal for fluorescence microscopy. Abrupt changes in pressure can be created with and without the presence of shear forces at the surface of cells. In primary cultures of human central nervous system cells, the cellular calcium response to shockwaves alone was negligible. Even when the applied pressure reached 15 atm, there was no damage or excitation, unless concomitant shear forces, peaking between 0.3 to 0.7 Pa, were present at the cell surface. The probability of cellular injury in response to a shockwave was low and cell survival was unaffected 20 hours after shockwave exposure. |
url |
http://europepmc.org/articles/PMC3387147?pdf=render |
work_keys_str_mv |
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