On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry
A simple, cheap and highly sensitive system with on-line preconcentration using solid-phase extraction in conjunction with flow injection spectrophotometry for the determination of tetracycline residues in milk samples is described. C18 was used as packing material i...
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Online Access: | http://www.mijst.mju.ac.th/vol2/418-430.pdf |
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doaj-9e1004fb75424309897eb6e651cf51bb2020-11-25T00:28:32ZengMaejo UniversityMaejo International Journal of Science and Technology1905-78732008-07-01202418430On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometryPrinya MasawatA simple, cheap and highly sensitive system with on-line preconcentration using solid-phase extraction in conjunction with flow injection spectrophotometry for the determination of tetracycline residues in milk samples is described. C18 was used as packing material in a designed minicolumn used for preconcentration of tetracyclines. Tetracycline standard or sample solutions were dissolved in a mixed buffer solution of pH 4.0 containing boric acid, citric acid and sodium phosphate, then loaded to the minicolumn for 6 min followed by elution with a solution containing methanol : mixed buffer solution (40:60 by volume) of pH 6.5 The absorbance of the eluate was measured at 370 nm. The calibration graph was linear in the range of 0.20-1.00, 0.20-4.00, and 0.20-1.00 mg L-1 for tetracycline (TC), oxytetracycline (OTC), and chlortetracycline (CTC) respectively. The limits of detection were 0.08, 0.10, and 0.09mg L-1 for TC, OTC, and CTC respectively. Relative standard deviations for 20 replicated determinations of 0.20, 0.40, and 0.60 mg L-1 of TC were 7.03, 7.23, and 6.55 % respectively. Per cent recoveries for four commercial types of milk: U.H.T., pasteurised, raw, and sterilised milk were in the range of 86–109 (TC), 90–109 (OTC), and 89–108 (CTC). The sample throughput was 6 h-1. http://www.mijst.mju.ac.th/vol2/418-430.pdftetracyclineoxytetracyclinechlortetracyclinetetracycline residuessolid-phase extraction flow injection spectrophotometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Prinya Masawat |
spellingShingle |
Prinya Masawat On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry Maejo International Journal of Science and Technology tetracycline oxytetracycline chlortetracycline tetracycline residues solid-phase extraction flow injection spectrophotometry |
author_facet |
Prinya Masawat |
author_sort |
Prinya Masawat |
title |
On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry |
title_short |
On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry |
title_full |
On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry |
title_fullStr |
On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry |
title_full_unstemmed |
On-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry |
title_sort |
on-line preconcentration and determination of tetracycline residues in milk using solid-phase extraction in conjunction with flow injection spectrophotometry |
publisher |
Maejo University |
series |
Maejo International Journal of Science and Technology |
issn |
1905-7873 |
publishDate |
2008-07-01 |
description |
A simple, cheap and highly sensitive system with on-line preconcentration using solid-phase extraction in conjunction with flow injection spectrophotometry for the determination of tetracycline residues in milk samples is described. C18 was used as packing material in a designed minicolumn used for preconcentration of tetracyclines. Tetracycline standard or sample solutions were dissolved in a mixed buffer solution of pH 4.0 containing boric acid, citric acid and sodium phosphate, then loaded to the minicolumn for 6 min followed by elution with a solution containing methanol : mixed buffer solution (40:60 by volume) of pH 6.5 The absorbance of the eluate was measured at 370 nm. The calibration graph was linear in the range of 0.20-1.00, 0.20-4.00, and 0.20-1.00 mg L-1 for tetracycline (TC), oxytetracycline (OTC), and chlortetracycline (CTC) respectively. The limits of detection were 0.08, 0.10, and 0.09mg L-1 for TC, OTC, and CTC respectively. Relative standard deviations for 20 replicated determinations of 0.20, 0.40, and 0.60 mg L-1 of TC were 7.03, 7.23, and 6.55 % respectively. Per cent recoveries for four commercial types of milk: U.H.T., pasteurised, raw, and sterilised milk were in the range of 86–109 (TC), 90–109 (OTC), and 89–108 (CTC). The sample throughput was 6 h-1. |
topic |
tetracycline oxytetracycline chlortetracycline tetracycline residues solid-phase extraction flow injection spectrophotometry |
url |
http://www.mijst.mju.ac.th/vol2/418-430.pdf |
work_keys_str_mv |
AT prinyamasawat onlinepreconcentrationanddeterminationoftetracyclineresiduesinmilkusingsolidphaseextractioninconjunctionwithflowinjectionspectrophotometry |
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