Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania major
Abstract Background Leishmaniasis, a disease caused by parasites of the genus Leishmania, infects roughly 12 million people worldwide, with about two million new cases per year. Prohibitins (PHBs) are highly conserved proteins belonging to the stomatin-prohibitin flotillin-HflC/K (SPFH) protein supe...
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doaj-9e139f07d64b4ed1af10076467173e042020-11-25T01:23:27ZengBMCParasites & Vectors1756-33052018-12-0111111410.1186/s13071-018-3195-8Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania majorTeresa Cruz-Bustos0Ana Karina Ibarrola-Vannucci1Isabel Díaz-Lozano2José Luis Ramírez3A. Osuna4Department of Parasitology, Faculty of Science, University of GranadaDepartment of Parasitology, Faculty of Science, University of GranadaDepartment of Parasitology, Faculty of Science, University of GranadaInstituto de Estudios Avanzados, (IDEA)Department of Parasitology, Faculty of Science, University of GranadaAbstract Background Leishmaniasis, a disease caused by parasites of the genus Leishmania, infects roughly 12 million people worldwide, with about two million new cases per year. Prohibitins (PHBs) are highly conserved proteins belonging to the stomatin-prohibitin flotillin-HflC/K (SPFH) protein superfamily. In this study, we examine the potential functions of two proteins of Leishmania major, PHB1 and PHB2, as well as how they might help protect the protozoan against oxidative stress. Results By immunolocalization in the parasite cells, PHB1 appeared in the mitochondria and plasma membrane, whereas PHB2 was grouped in the nucleus. When Leishmania cells were under oxidative stress, PHB1 migrates towards the plasma membrane and the paraxial rod, while PHB2 remained in the nucleus and near the kinetoplast. PHB1 presented higher mRNA levels than PHB2 in the amastigotes and the infective metacyclic forms. The mRNA expression of both prohibitins was affected by the presence of the Fe3+ ion. PHBs inhibited the Fenton reaction, where reactive oxygen species could nick DNA, implying that they play a crucial role in controlling oxidative stress. Conclusions Here, we propose that PHBs may help to protect membranes and DNA against superoxide ions, thus enhancing the survival capacity of the protozoan by controlling the ROS within the phagosome of the macrophages where the parasite multiplies.http://link.springer.com/article/10.1186/s13071-018-3195-8Leishmania majorProhibitin 1Prohibitin 2Oxidative stressImmunochemistryFunctionality |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Teresa Cruz-Bustos Ana Karina Ibarrola-Vannucci Isabel Díaz-Lozano José Luis Ramírez A. Osuna |
spellingShingle |
Teresa Cruz-Bustos Ana Karina Ibarrola-Vannucci Isabel Díaz-Lozano José Luis Ramírez A. Osuna Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania major Parasites & Vectors Leishmania major Prohibitin 1 Prohibitin 2 Oxidative stress Immunochemistry Functionality |
author_facet |
Teresa Cruz-Bustos Ana Karina Ibarrola-Vannucci Isabel Díaz-Lozano José Luis Ramírez A. Osuna |
author_sort |
Teresa Cruz-Bustos |
title |
Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania major |
title_short |
Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania major |
title_full |
Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania major |
title_fullStr |
Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania major |
title_full_unstemmed |
Characterization and functionality of two members of the SPFH protein superfamily, prohibitin 1 and 2 in Leishmania major |
title_sort |
characterization and functionality of two members of the spfh protein superfamily, prohibitin 1 and 2 in leishmania major |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2018-12-01 |
description |
Abstract Background Leishmaniasis, a disease caused by parasites of the genus Leishmania, infects roughly 12 million people worldwide, with about two million new cases per year. Prohibitins (PHBs) are highly conserved proteins belonging to the stomatin-prohibitin flotillin-HflC/K (SPFH) protein superfamily. In this study, we examine the potential functions of two proteins of Leishmania major, PHB1 and PHB2, as well as how they might help protect the protozoan against oxidative stress. Results By immunolocalization in the parasite cells, PHB1 appeared in the mitochondria and plasma membrane, whereas PHB2 was grouped in the nucleus. When Leishmania cells were under oxidative stress, PHB1 migrates towards the plasma membrane and the paraxial rod, while PHB2 remained in the nucleus and near the kinetoplast. PHB1 presented higher mRNA levels than PHB2 in the amastigotes and the infective metacyclic forms. The mRNA expression of both prohibitins was affected by the presence of the Fe3+ ion. PHBs inhibited the Fenton reaction, where reactive oxygen species could nick DNA, implying that they play a crucial role in controlling oxidative stress. Conclusions Here, we propose that PHBs may help to protect membranes and DNA against superoxide ions, thus enhancing the survival capacity of the protozoan by controlling the ROS within the phagosome of the macrophages where the parasite multiplies. |
topic |
Leishmania major Prohibitin 1 Prohibitin 2 Oxidative stress Immunochemistry Functionality |
url |
http://link.springer.com/article/10.1186/s13071-018-3195-8 |
work_keys_str_mv |
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