| Summary: | Xuesen Li,1 Na Li,2 Qinghui Niu,3 Haibin Zhu,4 Zhijie Wang,1 Qingxian Hou1 1Department of Spine Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, People’s Republic of China; 2Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, People’s Republic of China; 3Department of Hepatic Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, People’s Republic of China; 4Department of Orthopedics and Traumatology, Traditional Chinese Medical Hospital of Huangdao District, Qingdao, Shandong 266500, People’s Republic of ChinaCorrespondence: Qingxian HouDepartment of Spine Surgery, The Affiliated Hospital of Qingdao University, No. 1677, Wutaishan Road, Qingdao, Shandong 266555, People’s Republic of ChinaTel/Fax +86 532 82919525Email qingxiar547@163.comPurpose: Osteosarcoma (OS) is an invasive bone tumor that primarily affects children and adolescents. MicroRNA-629 (miR-629) acts as an oncogene involved in the development of various cancers. This study aims to reveal the clinical significance and biological function of miR-629 in OS.Patients and Methods: The levels of miR-629 expression in tissues and cells were detected through quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was used to evaluate the relationship between miR-621 expression and clinical parameters in patients with OS. Survival analysis was performed by the Kaplan–Meier method. Cox regression analysis of the effect of miR-629 expression on the prognosis of OS patients. CCK-8 and Transwell experiments were used to demonstrate the effect of miR-629 on OS cell function.Results: Compared with the controls, miR-629 levels were significantly elevated in patients with OS (P < 0.001), Furthermore, miR-629 upregulation showed significantly associated with clinical stage (P = 0.011), distant metastasis (P = 0.003) and poor survival (log rank test, P = 0.013) in OS patients. miR-629 might be a potential prognostic biomarker for OS (HR = 2.890, 95% CI = 1.126– 7.416, P = 0.027). Cell function experiments proved that the high expression of miR-629 promoted cell proliferation, migration, and invasion of OS.Conclusion: All experimental results demonstrated that miR-629 as an oncogene promotes the tumor cell growth, migration and invasion of OS, and miR-629 may act as a novel prognostic biomarker and therapeutic target for patients with this malignant tumor.Keywords: miR-629, prognosis, progression, osteosarcoma
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