IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK
Our paper presents new aspects regarding the micropropagation of Gisela 5 cherry rootstock. In the multiplication stage, modified MS media supplemented with 0.3 and 0.5 mg/l BAP as well as two types of gelling agents were tested: 3 g/l Plant Agar and 50 g/l wheat starch. The media supplemented with...
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Research Institute for Fruit Growing Pitesti
2013-12-01
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doaj-9e1f2cc046044ae090ab8c0b92cacb542020-11-24T23:50:21ZengResearch Institute for Fruit Growing Pitesti Fruit Growing Research2286-03042286-03042013-12-01XXIX104109IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCKDoina Clapa0Alexandru Firea 1Manuela Simu2Vladut Codrut Horga3Research Station for Fruit Growing Cluj, RomaniaResearch Station for Fruit Growing Cluj, RomaniaResearch Station for Fruit Growing Cluj, RomaniaBanat’s University of Agricultural Sciences and Veterinary Medicine Timişoara, RomaniaOur paper presents new aspects regarding the micropropagation of Gisela 5 cherry rootstock. In the multiplication stage, modified MS media supplemented with 0.3 and 0.5 mg/l BAP as well as two types of gelling agents were tested: 3 g/l Plant Agar and 50 g/l wheat starch. The media supplemented with 0.3 mg/l BAP and gelled with 3 g/l Plant Agar provided the highest proliferation rates (36.90 shoots/plantlet) and well-developed, high quality plantlets, whereas media gelled with starch also proved to be suitable but they caused deformations. In the in vitro rooting stage, MS media supplemented with 1 mg/l IBA provided 82.5 % rooting percentage and the plantlets were well developed. Ex vitro acclimatization of the plantlets rooted in vitro was successful in float hydroculture. Direct ex vitro rooting of shoots in floating perlite was also successful, with rooting pecentages exceeding 60 % but the plants were lesser developed as compared to the ones obtained by in vitro rooting and subsequent ex vitro acclimatization.http://www.icdp.ro/publicatii/lucrari%202013/20.Clapa%20Doina.pdftissue culturePrunus canencensvitro acclimatizationfloat hydrocultureperlit |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Doina Clapa Alexandru Firea Manuela Simu Vladut Codrut Horga |
spellingShingle |
Doina Clapa Alexandru Firea Manuela Simu Vladut Codrut Horga IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK Fruit Growing Research tissue culture Prunus canencens vitro acclimatization float hydroculture perlit |
author_facet |
Doina Clapa Alexandru Firea Manuela Simu Vladut Codrut Horga |
author_sort |
Doina Clapa |
title |
IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK |
title_short |
IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK |
title_full |
IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK |
title_fullStr |
IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK |
title_full_unstemmed |
IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK |
title_sort |
in vitro propagation of gisela 5 cherry rootstock |
publisher |
Research Institute for Fruit Growing Pitesti |
series |
Fruit Growing Research |
issn |
2286-0304 2286-0304 |
publishDate |
2013-12-01 |
description |
Our paper presents new aspects regarding the micropropagation of Gisela 5 cherry rootstock. In the multiplication stage, modified MS media supplemented with 0.3 and 0.5 mg/l BAP as well as two types of gelling agents were tested: 3 g/l Plant Agar and 50 g/l wheat starch. The media supplemented with 0.3 mg/l BAP and gelled with 3 g/l Plant Agar provided the highest proliferation rates (36.90 shoots/plantlet) and well-developed, high quality plantlets, whereas media gelled with starch also proved to be suitable but they caused deformations. In the in vitro rooting stage, MS media supplemented with 1 mg/l IBA provided 82.5 % rooting percentage and the plantlets were well developed. Ex vitro acclimatization of the plantlets rooted in vitro was successful in float hydroculture. Direct ex vitro rooting of shoots in floating perlite was also successful, with rooting pecentages exceeding 60 % but the plants were lesser developed as compared to the ones obtained by in vitro rooting and subsequent ex vitro acclimatization. |
topic |
tissue culture Prunus canencens vitro acclimatization float hydroculture perlit |
url |
http://www.icdp.ro/publicatii/lucrari%202013/20.Clapa%20Doina.pdf |
work_keys_str_mv |
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1725478965611593728 |