A different insight into blood coagulation in vitro
Objectives: The known model of blood coagulation involves a series of zymogen activation reaction sequences. At each stage, a zymogen is converted to an active protease by cleavage of one or more peptide bonds in the precursor molecule. The aim of this study was to investigate amino acid profiles du...
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2010-12-01
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doaj-9e1fccdef2d74a94af1e843c9e2f6e1a2021-09-02T16:20:21ZengModestum Publishing LTDJournal of Clinical and Experimental Investigations1309-85781309-66212010-12-0113173176A different insight into blood coagulation in vitroHanım KarahanBeri Hocaoğlu BozarslanBirgül IşıkM. Kemal BaşaralıSelvi KelekçiOsman EvliyaoğluObjectives: The known model of blood coagulation involves a series of zymogen activation reaction sequences. At each stage, a zymogen is converted to an active protease by cleavage of one or more peptide bonds in the precursor molecule. The aim of this study was to investigate amino acid profiles during coagulation process in different conditions in vitro.Methods: Samples of serum and plasma (treated by EDTA or citrate) were obtained from healthy donors and from patients with Phenylketonuria (PKU). Amino acid profiles analyzed with reverse phase HPLC column.Results: There were no differences between two plasma amino acid levels which were obtained by EDTA and acid citrate (p>0.05). Serum aspartate (asp), glutamate (glu), serine (ser), histidine (his) and phenylalanine (phe) levels were significantly higher in serum than plasma (p<0.05). This significant difference was not observed in patients with PKU.Conclusion: As a result the enzymatic reactions of coagulation process generate some aminoacids and these reactions take place in an appropriate chemical microenvironment. This microenvironment can be used to clarify the stages of coagulation cascades with further studies. J Clin Exp Invest 2010; 1(3): 173-176http://jceionline.org/upload/sayi/3/JCEI-00629.pdfCoagulationamino acidsPhenylketonuriain vitro |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hanım Karahan Beri Hocaoğlu Bozarslan Birgül Işık M. Kemal Başaralı Selvi Kelekçi Osman Evliyaoğlu |
spellingShingle |
Hanım Karahan Beri Hocaoğlu Bozarslan Birgül Işık M. Kemal Başaralı Selvi Kelekçi Osman Evliyaoğlu A different insight into blood coagulation in vitro Journal of Clinical and Experimental Investigations Coagulation amino acids Phenylketonuria in vitro |
author_facet |
Hanım Karahan Beri Hocaoğlu Bozarslan Birgül Işık M. Kemal Başaralı Selvi Kelekçi Osman Evliyaoğlu |
author_sort |
Hanım Karahan |
title |
A different insight into blood coagulation in vitro |
title_short |
A different insight into blood coagulation in vitro |
title_full |
A different insight into blood coagulation in vitro |
title_fullStr |
A different insight into blood coagulation in vitro |
title_full_unstemmed |
A different insight into blood coagulation in vitro |
title_sort |
different insight into blood coagulation in vitro |
publisher |
Modestum Publishing LTD |
series |
Journal of Clinical and Experimental Investigations |
issn |
1309-8578 1309-6621 |
publishDate |
2010-12-01 |
description |
Objectives: The known model of blood coagulation involves a series of zymogen activation reaction sequences. At each stage, a zymogen is converted to an active protease by cleavage of one or more peptide bonds in the precursor molecule. The aim of this study was to investigate amino acid profiles during coagulation process in different conditions in vitro.Methods: Samples of serum and plasma (treated by EDTA or citrate) were obtained from healthy donors and from patients with Phenylketonuria (PKU). Amino acid profiles analyzed with reverse phase HPLC column.Results: There were no differences between two plasma amino acid levels which were obtained by EDTA and acid citrate (p>0.05). Serum aspartate (asp), glutamate (glu), serine (ser), histidine (his) and phenylalanine (phe) levels were significantly higher in serum than plasma (p<0.05). This significant difference was not observed in patients with PKU.Conclusion: As a result the enzymatic reactions of coagulation process generate some aminoacids and these reactions take place in an appropriate chemical microenvironment. This microenvironment can be used to clarify the stages of coagulation cascades with further studies. J Clin Exp Invest 2010; 1(3): 173-176 |
topic |
Coagulation amino acids Phenylketonuria in vitro |
url |
http://jceionline.org/upload/sayi/3/JCEI-00629.pdf |
work_keys_str_mv |
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