Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)
Abstract Background Entomopathogenic fungi are widely distributed and well described within the fungal kingdom. This study reports the isolation, characterization, and virulence of 4 Purpureocillium lilacinum isolates against the sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodi...
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doaj-9e4b85ec15a54970b5f70f98ec7b49762021-02-07T12:23:48ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422021-02-013111910.1186/s41938-021-00372-yMorphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)Tingfei Sun0Jianhui Wu1Shaukat Ali2Key Laboratory for Biopesticide Creation and Application of Guangdong Province, College of Plant Protection, South China Agricultural UniversityKey Laboratory for Biopesticide Creation and Application of Guangdong Province, College of Plant Protection, South China Agricultural UniversityKey Laboratory for Biopesticide Creation and Application of Guangdong Province, College of Plant Protection, South China Agricultural UniversityAbstract Background Entomopathogenic fungi are widely distributed and well described within the fungal kingdom. This study reports the isolation, characterization, and virulence of 4 Purpureocillium lilacinum isolates against the sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Results Four strains of Purpureocillium lilacinum (XI-1, XI-4, XI-5, and J27) were isolated from soil samples from different localities of China. The morphological studies observed that four strains showed essentially the same morphological characteristics. After 7 days of cultivation, the colonies were purple, round, and bulged. Conidia were single-celled, oval to spindle-shaped, chain-like, and the spore size was about 2.0–2.3 × 3.1–4.0 μm. The genome-based identification results showed that ITS sequences of XI-1 (GenBank accession # MW386433), XI-4 (GenBank accession # MW386434), XI-5 (GenBank accession # MW386435), and J27 (GenBank accession # MW386436) were similar to another P. lilacinum. The newly identified strains of P. lilacinum proved pathogenicity to B. tabaci under laboratory conditions. In addition, the P. lilacinum isolate XI-5 was the most virulent one against different nymphal instars of whitefly having median lethal concentration (LC50) values of 4.99 × 106, 4.82 × 105, and 2.85 × 106 conidia/ml, respectively, 7 days post application. Conclusion The newly isolated strains of P. lilacinum can be developed as a potential biopesticide against the whitefly although extensive field bioassays as well as development of proper formulation are still required.https://doi.org/10.1186/s41938-021-00372-yEntomopathogenic fungiPurpureocillium lilacinumIsolationBemisia tabaciVirulence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tingfei Sun Jianhui Wu Shaukat Ali |
spellingShingle |
Tingfei Sun Jianhui Wu Shaukat Ali Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) Egyptian Journal of Biological Pest Control Entomopathogenic fungi Purpureocillium lilacinum Isolation Bemisia tabaci Virulence |
author_facet |
Tingfei Sun Jianhui Wu Shaukat Ali |
author_sort |
Tingfei Sun |
title |
Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) |
title_short |
Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) |
title_full |
Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) |
title_fullStr |
Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) |
title_full_unstemmed |
Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) |
title_sort |
morphological and molecular identification of four purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, bemisia tabaci (genn.) (hemiptera: aleyrodidae) |
publisher |
SpringerOpen |
series |
Egyptian Journal of Biological Pest Control |
issn |
2536-9342 |
publishDate |
2021-02-01 |
description |
Abstract Background Entomopathogenic fungi are widely distributed and well described within the fungal kingdom. This study reports the isolation, characterization, and virulence of 4 Purpureocillium lilacinum isolates against the sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Results Four strains of Purpureocillium lilacinum (XI-1, XI-4, XI-5, and J27) were isolated from soil samples from different localities of China. The morphological studies observed that four strains showed essentially the same morphological characteristics. After 7 days of cultivation, the colonies were purple, round, and bulged. Conidia were single-celled, oval to spindle-shaped, chain-like, and the spore size was about 2.0–2.3 × 3.1–4.0 μm. The genome-based identification results showed that ITS sequences of XI-1 (GenBank accession # MW386433), XI-4 (GenBank accession # MW386434), XI-5 (GenBank accession # MW386435), and J27 (GenBank accession # MW386436) were similar to another P. lilacinum. The newly identified strains of P. lilacinum proved pathogenicity to B. tabaci under laboratory conditions. In addition, the P. lilacinum isolate XI-5 was the most virulent one against different nymphal instars of whitefly having median lethal concentration (LC50) values of 4.99 × 106, 4.82 × 105, and 2.85 × 106 conidia/ml, respectively, 7 days post application. Conclusion The newly isolated strains of P. lilacinum can be developed as a potential biopesticide against the whitefly although extensive field bioassays as well as development of proper formulation are still required. |
topic |
Entomopathogenic fungi Purpureocillium lilacinum Isolation Bemisia tabaci Virulence |
url |
https://doi.org/10.1186/s41938-021-00372-y |
work_keys_str_mv |
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