SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease
Abstract Background Atherosclerosis is the primary cause of coronary artery disease (CAD), and stearoyl‐CoA desaturase (SCD) is associated with atherosclerosis. However, the associations between variants of SCD and CAD have not yet been decided. Methods This study analyzed SCD rs41290540 single‐nucl...
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2020-03-01
|
Series: | Molecular Genetics & Genomic Medicine |
Subjects: | |
Online Access: | https://doi.org/10.1002/mgg3.1136 |
id |
doaj-9e54ec917bbd44aeb64b63226f6b369e |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhou Liu Xiaojian Yin Hui Mai Guangning Li Zhijun Lin Wanxin Jie Kanglan Li Haihong Zhou Shouchao Wei Li Hu Wanjuan Peng Jiajing Lin Feng Yao Hua Tao Xing‐dong Xiong Keshen Li |
spellingShingle |
Zhou Liu Xiaojian Yin Hui Mai Guangning Li Zhijun Lin Wanxin Jie Kanglan Li Haihong Zhou Shouchao Wei Li Hu Wanjuan Peng Jiajing Lin Feng Yao Hua Tao Xing‐dong Xiong Keshen Li SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease Molecular Genetics & Genomic Medicine 3′‐untranslated region coronary artery disease microRNA single‐nucleotide polymorphism stearoyl‐CoA desaturase |
author_facet |
Zhou Liu Xiaojian Yin Hui Mai Guangning Li Zhijun Lin Wanxin Jie Kanglan Li Haihong Zhou Shouchao Wei Li Hu Wanjuan Peng Jiajing Lin Feng Yao Hua Tao Xing‐dong Xiong Keshen Li |
author_sort |
Zhou Liu |
title |
SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease |
title_short |
SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease |
title_full |
SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease |
title_fullStr |
SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease |
title_full_unstemmed |
SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease |
title_sort |
scd rs41290540 single‐nucleotide polymorphism modifies mir‐498 binding and is associated with a decreased risk of coronary artery disease |
publisher |
Wiley |
series |
Molecular Genetics & Genomic Medicine |
issn |
2324-9269 |
publishDate |
2020-03-01 |
description |
Abstract Background Atherosclerosis is the primary cause of coronary artery disease (CAD), and stearoyl‐CoA desaturase (SCD) is associated with atherosclerosis. However, the associations between variants of SCD and CAD have not yet been decided. Methods This study analyzed SCD rs41290540 single‐nucleotide polymorphism (SNP) in the 3′‐untranslated region for an association with a risk of CAD among the Chinese Han population. CAD patients and controls were genotyped for SNP rs41290540 in SCD by SNaPshot. The binding affinity of miR‐498 to rs41290540 was determined by a luciferase assay, and SCD expression was assessed using Western blot. Results A total of 969 CAD patients and 1,095 control subjects were involved in this study. The SCD rs41290540CC genotype is associated with a decreased risk of CAD compared with the AA genotype. Furthermore, the CC genotype is associated with lower serum total cholesterol (TC). Western blot analysis demonstrated that miR‐498 suppressed the expression of SCD. A luciferase assay confirmed that rs41290540 A>C variation in the SCD 3′UTR inhibits miR‐498 binding. Conclusion This study demonstrates that the SCD rs41290540 may be associated with a decreased risk of CAD, lower serum TC, and decreased miR‐498 binding. |
topic |
3′‐untranslated region coronary artery disease microRNA single‐nucleotide polymorphism stearoyl‐CoA desaturase |
url |
https://doi.org/10.1002/mgg3.1136 |
work_keys_str_mv |
AT zhouliu scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT xiaojianyin scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT huimai scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT guangningli scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT zhijunlin scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT wanxinjie scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT kanglanli scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT haihongzhou scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT shouchaowei scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT lihu scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT wanjuanpeng scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT jiajinglin scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT fengyao scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT huatao scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT xingdongxiong scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease AT keshenli scdrs41290540singlenucleotidepolymorphismmodifiesmir498bindingandisassociatedwithadecreasedriskofcoronaryarterydisease |
_version_ |
1716776717247315968 |
spelling |
doaj-9e54ec917bbd44aeb64b63226f6b369e2020-11-24T21:02:04ZengWileyMolecular Genetics & Genomic Medicine2324-92692020-03-0183n/an/a10.1002/mgg3.1136SCD rs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery diseaseZhou Liu0Xiaojian Yin1Hui Mai2Guangning Li3Zhijun Lin4Wanxin Jie5Kanglan Li6Haihong Zhou7Shouchao Wei8Li Hu9Wanjuan Peng10Jiajing Lin11Feng Yao12Hua Tao13Xing‐dong Xiong14Keshen Li15Department of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Huadu District People’s Hospital Southern Medical University Guangzhou ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaCardiovascular Medicine Center Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaInstitute of Aging Research Guangdong Medical University Dongguan ChinaDepartment of Neurology Guangdong Key Laboratory of Age‐Related Cardiac and Cerebral Diseases Institute of Neurology Affiliated Hospital of Guangdong Medical University Zhanjiang ChinaAbstract Background Atherosclerosis is the primary cause of coronary artery disease (CAD), and stearoyl‐CoA desaturase (SCD) is associated with atherosclerosis. However, the associations between variants of SCD and CAD have not yet been decided. Methods This study analyzed SCD rs41290540 single‐nucleotide polymorphism (SNP) in the 3′‐untranslated region for an association with a risk of CAD among the Chinese Han population. CAD patients and controls were genotyped for SNP rs41290540 in SCD by SNaPshot. The binding affinity of miR‐498 to rs41290540 was determined by a luciferase assay, and SCD expression was assessed using Western blot. Results A total of 969 CAD patients and 1,095 control subjects were involved in this study. The SCD rs41290540CC genotype is associated with a decreased risk of CAD compared with the AA genotype. Furthermore, the CC genotype is associated with lower serum total cholesterol (TC). Western blot analysis demonstrated that miR‐498 suppressed the expression of SCD. A luciferase assay confirmed that rs41290540 A>C variation in the SCD 3′UTR inhibits miR‐498 binding. Conclusion This study demonstrates that the SCD rs41290540 may be associated with a decreased risk of CAD, lower serum TC, and decreased miR‐498 binding.https://doi.org/10.1002/mgg3.11363′‐untranslated regioncoronary artery diseasemicroRNAsingle‐nucleotide polymorphismstearoyl‐CoA desaturase |