Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015

Background: Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. Raw milk or dairy products that are produced from unpasteurized milk may contain virulent Coxiella burnetii. The objective of this study was to determine the prevalence rate of...

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Main Authors: Lida Etemadfar, Nemat Shams, Amin Jaidari
Format: Article
Language:fas
Published: Lorestan University of Medical Science 2017-10-01
Series:Yafteh
Subjects:
Online Access:http://yafte.lums.ac.ir/browse.php?a_code=A-10-1693-78&slc_lang=en&sid=1
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spelling doaj-9e633c7031d14f02b11d91c443e87f252020-11-25T02:44:25ZfasLorestan University of Medical ScienceYafteh1563-07732017-10-011924149Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015Lida Etemadfar0Nemat Shams1Amin Jaidari2 Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran ,Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran ,Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran Background: Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. Raw milk or dairy products that are produced from unpasteurized milk may contain virulent Coxiella burnetii. The objective of this study was to determine the prevalence rate of C. burnetii in raw and unpasteurized cow bulk tank milk samples of traditional domestic dairy products vendors in Khorramabad, Iran. Materials and Methods: In this cross - sectional study a total of 120 raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors were collected from October 2015 to November 2015 and tested for C. burnetii used a nested PCR assay. Results: In this survey, 9 out of 120 (7.5%) raw and unpasteurized cow bulk tank milk samples were found PCR positive for C. burnetii. Conclusion: The Results of this study indicate that raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors are an important source of C. burnetii infection in Khorramabad.http://yafte.lums.ac.ir/browse.php?a_code=A-10-1693-78&slc_lang=en&sid=1Nested PCR Q - fever Raw milk Khorramabad
collection DOAJ
language fas
format Article
sources DOAJ
author Lida Etemadfar
Nemat Shams
Amin Jaidari
spellingShingle Lida Etemadfar
Nemat Shams
Amin Jaidari
Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015
Yafteh
Nested PCR
Q - fever
Raw milk
Khorramabad
author_facet Lida Etemadfar
Nemat Shams
Amin Jaidari
author_sort Lida Etemadfar
title Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015
title_short Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015
title_full Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015
title_fullStr Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015
title_full_unstemmed Genomic Detection of Coxiella burnetii in Raw and Unpasteurized Cow Milk of Traditional domestic dairy products Vendors in Khorramabad, Lorestan Province in 2015
title_sort genomic detection of coxiella burnetii in raw and unpasteurized cow milk of traditional domestic dairy products vendors in khorramabad, lorestan province in 2015
publisher Lorestan University of Medical Science
series Yafteh
issn 1563-0773
publishDate 2017-10-01
description Background: Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. Raw milk or dairy products that are produced from unpasteurized milk may contain virulent Coxiella burnetii. The objective of this study was to determine the prevalence rate of C. burnetii in raw and unpasteurized cow bulk tank milk samples of traditional domestic dairy products vendors in Khorramabad, Iran. Materials and Methods: In this cross - sectional study a total of 120 raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors were collected from October 2015 to November 2015 and tested for C. burnetii used a nested PCR assay. Results: In this survey, 9 out of 120 (7.5%) raw and unpasteurized cow bulk tank milk samples were found PCR positive for C. burnetii. Conclusion: The Results of this study indicate that raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors are an important source of C. burnetii infection in Khorramabad.
topic Nested PCR
Q - fever
Raw milk
Khorramabad
url http://yafte.lums.ac.ir/browse.php?a_code=A-10-1693-78&slc_lang=en&sid=1
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AT nematshams genomicdetectionofcoxiellaburnetiiinrawandunpasteurizedcowmilkoftraditionaldomesticdairyproductsvendorsinkhorramabadlorestanprovincein2015
AT aminjaidari genomicdetectionofcoxiellaburnetiiinrawandunpasteurizedcowmilkoftraditionaldomesticdairyproductsvendorsinkhorramabadlorestanprovincein2015
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