Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis B
Summary: Infections with hepatitis B virus (HBV) can initiate chronic hepatitis and liver injury, causing more than 600,000 deaths each year worldwide. Current treatments for chronic hepatitis B are inadequate and leave an unmet need for immunotherapeutic approaches. We designed virus-like vesicles...
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doaj-9e64bffbe3444bc090c0083118c49c3a2020-11-24T21:55:22ZengElsevieriScience2589-00422019-11-0121391402Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis BTimur O. Yarovinsky0Stephen W. Mason1Manisha Menon2Marie M. Krady3Maria Haslip4Bhaskara R. Madina5Xianyong Ma6Safiehkhatoon Moshkani7Carolina Chiale8Anasuya Chattopadhyay Pal9Bijan Almassian10John K. Rose11Michael D. Robek12Valerian Nakaar13CaroGen Corporation, Farmington, CT 06032, USA; Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA; Corresponding authorCaroGen Corporation, Farmington, CT 06032, USACaroGen Corporation, Farmington, CT 06032, USACaroGen Corporation, Farmington, CT 06032, USACaroGen Corporation, Farmington, CT 06032, USACaroGen Corporation, Farmington, CT 06032, USACaroGen Corporation, Farmington, CT 06032, USADepartment of Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USADepartment of Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USADepartment of Pathology, Yale University School of Medicine, New Haven, CT 06510, USACaroGen Corporation, Farmington, CT 06032, USADepartment of Pathology, Yale University School of Medicine, New Haven, CT 06510, USADepartment of Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208, USACaroGen Corporation, Farmington, CT 06032, USA; Corresponding authorSummary: Infections with hepatitis B virus (HBV) can initiate chronic hepatitis and liver injury, causing more than 600,000 deaths each year worldwide. Current treatments for chronic hepatitis B are inadequate and leave an unmet need for immunotherapeutic approaches. We designed virus-like vesicles (VLV) as self-amplifying RNA replicons expressing three HBV antigens (polymerase, core, and middle surface) from a single vector (HBV-VLV) to break immune exhaustion despite persistent HBV replication. The HBV-VLV induces HBV-specific T cells in naive mice and renders them resistant to acute challenge with HBV. Using a chronic model of HBV infection, we demonstrate efficacy of HBV-VLV priming in combination with DNA booster immunization, as 40% of treated mice showed a decline of serum HBV surface antigen below the detection limit and marked reduction in liver HBV RNA accompanied by induction of HBsAg-specific CD8 T cells. These results warrant further evaluation of HBV-VLV for immunotherapy of chronic hepatitis B. : Immunology; Virology; Medical Microbiology Subject Areas: Immunology, Virology, Medical Microbiologyhttp://www.sciencedirect.com/science/article/pii/S2589004219304201 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Timur O. Yarovinsky Stephen W. Mason Manisha Menon Marie M. Krady Maria Haslip Bhaskara R. Madina Xianyong Ma Safiehkhatoon Moshkani Carolina Chiale Anasuya Chattopadhyay Pal Bijan Almassian John K. Rose Michael D. Robek Valerian Nakaar |
spellingShingle |
Timur O. Yarovinsky Stephen W. Mason Manisha Menon Marie M. Krady Maria Haslip Bhaskara R. Madina Xianyong Ma Safiehkhatoon Moshkani Carolina Chiale Anasuya Chattopadhyay Pal Bijan Almassian John K. Rose Michael D. Robek Valerian Nakaar Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis B iScience |
author_facet |
Timur O. Yarovinsky Stephen W. Mason Manisha Menon Marie M. Krady Maria Haslip Bhaskara R. Madina Xianyong Ma Safiehkhatoon Moshkani Carolina Chiale Anasuya Chattopadhyay Pal Bijan Almassian John K. Rose Michael D. Robek Valerian Nakaar |
author_sort |
Timur O. Yarovinsky |
title |
Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis B |
title_short |
Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis B |
title_full |
Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis B |
title_fullStr |
Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis B |
title_full_unstemmed |
Virus-like Vesicles Expressing Multiple Antigens for Immunotherapy of Chronic Hepatitis B |
title_sort |
virus-like vesicles expressing multiple antigens for immunotherapy of chronic hepatitis b |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2019-11-01 |
description |
Summary: Infections with hepatitis B virus (HBV) can initiate chronic hepatitis and liver injury, causing more than 600,000 deaths each year worldwide. Current treatments for chronic hepatitis B are inadequate and leave an unmet need for immunotherapeutic approaches. We designed virus-like vesicles (VLV) as self-amplifying RNA replicons expressing three HBV antigens (polymerase, core, and middle surface) from a single vector (HBV-VLV) to break immune exhaustion despite persistent HBV replication. The HBV-VLV induces HBV-specific T cells in naive mice and renders them resistant to acute challenge with HBV. Using a chronic model of HBV infection, we demonstrate efficacy of HBV-VLV priming in combination with DNA booster immunization, as 40% of treated mice showed a decline of serum HBV surface antigen below the detection limit and marked reduction in liver HBV RNA accompanied by induction of HBsAg-specific CD8 T cells. These results warrant further evaluation of HBV-VLV for immunotherapy of chronic hepatitis B. : Immunology; Virology; Medical Microbiology Subject Areas: Immunology, Virology, Medical Microbiology |
url |
http://www.sciencedirect.com/science/article/pii/S2589004219304201 |
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