Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC)
This work aimed at the detection and quantification of Total Coliforms and Escherichia coli in Igapó Lake, in order to evaluate the quality of these waters as proper or unfit for recreation, in addition to the genotypic characterization of virulence factors associated with EPEC and STEC by PCR. The...
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doaj-9e807460f88b4c6a9aaed98d0db1ae852020-11-25T00:20:56ZengUniversidade Estadual de LondrinaSemina : Ciências Biológicas e da Saúde1676-54351679-03672014-12-01352112010.5433/1679-0367.2014v35n2p1110375Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC)Paulo Alfonso Schuroff0Nicole Ribeiro de Lima1Tatiane das Neves Burgos2Angélica Marim Lopes3Jacinta Sanchez Pelayo4Universidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de Londrina, Departamento de Microbiologia, Laboratório de BacteriologiaThis work aimed at the detection and quantification of Total Coliforms and Escherichia coli in Igapó Lake, in order to evaluate the quality of these waters as proper or unfit for recreation, in addition to the genotypic characterization of virulence factors associated with EPEC and STEC by PCR. The study area was the Igapó Lake I, II, III and IV. Samples were collected monthly from March 2011 to February 2012. The technique used for the detection and quantification of Total Coliforms and E. coli was the Colilert chromogenic substrate. In the technique of PCR, the eae and bfp genes were tested to characterize the typical EPEC pathotype; stx1, stx2, eae and hlyA the STEC pathotype and the samples that presented only the eae gene were characterized as atypical EPEC. According to CONAMA Resolution 357/2005, it has been observed that only Igapó Lake III was rated inappropriate for primary contact recreation, while for secondary contact recreation, all lakes were considered appropriate. Moreover, a strong relationship between rainfall and E. coli indices in Igapó Lake can be observed, which in dry months the quantity drastically decreases, while in rainy months the opposite relationship was observed. Of the 97 strains of E. coli isolated, two had the eae gene (atypical EPEC). None of the isolates contained genes stx1, stx2, bfp and hlyA. Thus, we hope to educate the population and public agencies of the importance of microbiological monitoring of recreational waters to prevent outbreaks of waterborne infections.http://www.uel.br/revistas/uel/index.php/seminabio/article/view/15545Lago IgapóÁguas recreacionaisE. coliFatores de virulência |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paulo Alfonso Schuroff Nicole Ribeiro de Lima Tatiane das Neves Burgos Angélica Marim Lopes Jacinta Sanchez Pelayo |
spellingShingle |
Paulo Alfonso Schuroff Nicole Ribeiro de Lima Tatiane das Neves Burgos Angélica Marim Lopes Jacinta Sanchez Pelayo Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) Semina : Ciências Biológicas e da Saúde Lago Igapó Águas recreacionais E. coli Fatores de virulência |
author_facet |
Paulo Alfonso Schuroff Nicole Ribeiro de Lima Tatiane das Neves Burgos Angélica Marim Lopes Jacinta Sanchez Pelayo |
author_sort |
Paulo Alfonso Schuroff |
title |
Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) |
title_short |
Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) |
title_full |
Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) |
title_fullStr |
Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) |
title_full_unstemmed |
Microbiological water quality of Igapó Lake Londrina - PR and genotypic characterization of virulence factors associated with enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) |
title_sort |
microbiological water quality of igapó lake londrina - pr and genotypic characterization of virulence factors associated with enteropathogenic escherichia coli (epec) and shiga toxin-producing e. coli (stec) |
publisher |
Universidade Estadual de Londrina |
series |
Semina : Ciências Biológicas e da Saúde |
issn |
1676-5435 1679-0367 |
publishDate |
2014-12-01 |
description |
This work aimed at the detection and quantification of Total Coliforms and Escherichia coli in Igapó Lake, in order to evaluate the quality of these waters as proper or unfit for recreation, in addition to the genotypic characterization of virulence factors associated with EPEC and STEC by PCR. The study area was the Igapó Lake I, II, III and IV. Samples were collected monthly from March 2011 to February 2012. The technique used for the detection and quantification of Total Coliforms and E. coli was the Colilert chromogenic substrate. In the technique of PCR, the eae and bfp genes were tested to characterize the typical EPEC pathotype; stx1, stx2, eae and hlyA the STEC pathotype and the samples that presented only the eae gene were characterized as atypical EPEC. According to CONAMA Resolution 357/2005, it has been observed that only Igapó Lake III was rated inappropriate for primary contact recreation, while for secondary contact recreation, all lakes were considered appropriate. Moreover, a strong relationship between rainfall and E. coli indices in Igapó Lake can be observed, which in dry months the quantity drastically decreases, while in rainy months the opposite relationship was observed. Of the 97 strains of E. coli isolated, two had the eae gene (atypical EPEC). None of the isolates contained genes stx1, stx2, bfp and hlyA. Thus, we hope to educate the population and public agencies of the importance of microbiological monitoring of recreational waters to prevent outbreaks of waterborne infections. |
topic |
Lago Igapó Águas recreacionais E. coli Fatores de virulência |
url |
http://www.uel.br/revistas/uel/index.php/seminabio/article/view/15545 |
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