Novel Platinum-Porphyrin as Sensing Compound for Efficient Fluorescent and Electrochemical Detection of H₂O₂

• Main Authors: Eugenia Fagadar-Cosma, Nicoleta Plesu, Anca Lascu, Diana Anghel, Maria Cazacu, Catalin Ianasi, Gheorghe Fagadar-Cosma, Ion Fratilescu, Camelia Epuran
• Format: Article
• Language: English
• Published: MDPI AG 2020-04-01
• Series: Chemosensors
• Subjects: Pt-porphyrin; photoactive material; UV-vis spectroscopy; fluorimetry; electrochemistry; hydrogen peroxide detection
• Online Access: https://www.mdpi.com/2227-9040/8/2/29

Metalloporphyrins are highly recognized for their capacity to act as sensitive substances used in formulation of optical, fluorescent, and electrochemical sensors. A novel compound, namely Pt(II)-5,10,15,20-tetra-(4-allyloxy-phenyl) porphyrin, was synthesized by metalation with PtCl₂(PhCN)₂ of the corresponding porphyrin base and was fully characterized by UV-vis, fluorimetry, FT-IR, ¹H-NMR, and ¹³C-NMR methods. The fluorescence response of this Pt-porphyrin in the presence of different concentrations of hydrogen peroxide was investigated. Besides, modified glassy carbon electrodes with this Pt-porphyrin (Pt-Porf-GCE) were realized and several electrochemical characterizations were comparatively performed with bare glassy carbon electrodes (GCE), in the absence or presence of hydrogen peroxide. The Pt-porphyrin demonstrated to be a successful sensitive material for the detection of hydrogen peroxide both by fluorimetric method in a concentration range relevant for biological samples (1.05–3.9 × 10⁻⁷ M) and by electrochemical method, in a larger concentration range from 1 × 10⁻⁶ M to 5 × 10⁻⁵ M. Based on different methods, this Pt-porphyrin can cover detection in diverse fields, from medical tests to food and agricultural monitoring, proving high accuracy (correlation coefficients over 99%) in both fluorimetric and electrochemical measurements.