Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).
Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by...
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2019-01-01
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Online Access: | https://doi.org/10.1371/journal.pone.0223794 |
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doaj-9e9bfe44b9a64bba93296f1317f155f62021-03-03T21:09:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011410e022379410.1371/journal.pone.0223794Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).José E Guzmán-FloresLidia Steinemann-HernándezLuis E González de la VaraMarina Gavilanes-RuizTony RomeoAdrián F AlvarezDimitris GeorgellisMembrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by mass spectrometry analyses of Detergent Resistant Membranes (DRM). We report that at least three of the four E. coli flotillin homologous proteins were found to reside in DRM, along with 77 more proteins. Moreover, the proteomic data were validated by subcellular localization, using immunoblot assays and fluorescence microscopy of selected proteins. Our results confirm the existence of lipid raft-like microdomains in the inner membrane of E. coli and represent the first comprehensive profiling of proteins in these bacterial membrane platforms.https://doi.org/10.1371/journal.pone.0223794 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José E Guzmán-Flores Lidia Steinemann-Hernández Luis E González de la Vara Marina Gavilanes-Ruiz Tony Romeo Adrián F Alvarez Dimitris Georgellis |
spellingShingle |
José E Guzmán-Flores Lidia Steinemann-Hernández Luis E González de la Vara Marina Gavilanes-Ruiz Tony Romeo Adrián F Alvarez Dimitris Georgellis Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM). PLoS ONE |
author_facet |
José E Guzmán-Flores Lidia Steinemann-Hernández Luis E González de la Vara Marina Gavilanes-Ruiz Tony Romeo Adrián F Alvarez Dimitris Georgellis |
author_sort |
José E Guzmán-Flores |
title |
Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM). |
title_short |
Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM). |
title_full |
Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM). |
title_fullStr |
Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM). |
title_full_unstemmed |
Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM). |
title_sort |
proteomic analysis of escherichia coli detergent-resistant membranes (drm). |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by mass spectrometry analyses of Detergent Resistant Membranes (DRM). We report that at least three of the four E. coli flotillin homologous proteins were found to reside in DRM, along with 77 more proteins. Moreover, the proteomic data were validated by subcellular localization, using immunoblot assays and fluorescence microscopy of selected proteins. Our results confirm the existence of lipid raft-like microdomains in the inner membrane of E. coli and represent the first comprehensive profiling of proteins in these bacterial membrane platforms. |
url |
https://doi.org/10.1371/journal.pone.0223794 |
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