Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers
Abstract An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (A...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-01-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-020-79702-3 |
id |
doaj-9e9d3543db6149498cb8f68e9f626e45 |
---|---|
record_format |
Article |
spelling |
doaj-9e9d3543db6149498cb8f68e9f626e452021-01-24T12:30:14ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111510.1038/s41598-020-79702-3Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayersJessie L. Webber0Rashin Namivandi-Zangeneh1Sławomir Drozdek2Kazimiera A. Wilk3Cyrille Boyer4Edgar H. H. Wong5Bronwyn H. Bradshaw-Hajek6Marta Krasowska7David A. Beattie8Future Industries Institute, University of South AustraliaCentre for Advanced Macromolecular Design and Australian Centre for NanoMedicine, School of Chemical Engineering, University of New South WalesFuture Industries Institute, University of South AustraliaDepartment of Engineering and Technology of Chemical Processes, Faculty of Chemistry, Wrocław University of Science and TechnologyCentre for Advanced Macromolecular Design and Australian Centre for NanoMedicine, School of Chemical Engineering, University of New South WalesCentre for Advanced Macromolecular Design and Australian Centre for NanoMedicine, School of Chemical Engineering, University of New South WalesUniSA STEM, University of South AustraliaFuture Industries Institute, University of South AustraliaFuture Industries Institute, University of South AustraliaAbstract An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm−2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.https://doi.org/10.1038/s41598-020-79702-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jessie L. Webber Rashin Namivandi-Zangeneh Sławomir Drozdek Kazimiera A. Wilk Cyrille Boyer Edgar H. H. Wong Bronwyn H. Bradshaw-Hajek Marta Krasowska David A. Beattie |
spellingShingle |
Jessie L. Webber Rashin Namivandi-Zangeneh Sławomir Drozdek Kazimiera A. Wilk Cyrille Boyer Edgar H. H. Wong Bronwyn H. Bradshaw-Hajek Marta Krasowska David A. Beattie Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers Scientific Reports |
author_facet |
Jessie L. Webber Rashin Namivandi-Zangeneh Sławomir Drozdek Kazimiera A. Wilk Cyrille Boyer Edgar H. H. Wong Bronwyn H. Bradshaw-Hajek Marta Krasowska David A. Beattie |
author_sort |
Jessie L. Webber |
title |
Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers |
title_short |
Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers |
title_full |
Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers |
title_fullStr |
Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers |
title_full_unstemmed |
Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers |
title_sort |
incorporation and antimicrobial activity of nisin z within carrageenan/chitosan multilayers |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-01-01 |
description |
Abstract An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm−2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors. |
url |
https://doi.org/10.1038/s41598-020-79702-3 |
work_keys_str_mv |
AT jessielwebber incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT rashinnamivandizangeneh incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT sławomirdrozdek incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT kazimieraawilk incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT cyrilleboyer incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT edgarhhwong incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT bronwynhbradshawhajek incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT martakrasowska incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT davidabeattie incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers |
_version_ |
1724325705221668864 |