Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material
Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepare...
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doaj-9eb3e8cbc36a4db28be60ab9033afbc62020-11-25T02:36:39ZengMDPI AGPharmaceutics1999-49232020-05-011248248210.3390/pharmaceutics12050482Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic MaterialJosé Antonio Lebrón0Francisco José Ostos1Manuel López-López2María Luisa Moyá3Carlos Sales4Encarnación García5Clara Beatriz García-Calderón6Margarita García-Calderón7María José Peña-Gómez8Iván V. Rosado9Fernando R. Balestra10Pablo Huertas11Pilar López-Cornejo12Department of Physical Chemistry, Faculty of Chemistry, University of Seville, c/Prof. García González nº 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, c/Prof. García González nº 1, 41012 Seville, SpainDepartment of Chemical Engineering, Physical Chemistry and Materials Science, Faculty of Experimental Sciences, Campus de El Carmen, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, c/Prof. García González nº 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, c/Prof. García González nº 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, c/Prof. García González nº 1, 41012 Seville, SpainInstitute of Biomedicine of Seville (IBIS), University Hospital Virgen del Rocio/CSIC/University of Seville, Avda. Manuel Siurot s/n, 41013 Seville, SpainDepartment of Vegetal Biochemistry and Molecular Biology, Faculty of Chemistry, c/Prof. García González nº 1, 41012 Seville, SpainInstitute of Biomedicine of Seville (IBIS), University Hospital Virgen del Rocio/CSIC/University of Seville, Avda. Manuel Siurot s/n, 41013 Seville, SpainInstitute of Biomedicine of Seville (IBIS), University Hospital Virgen del Rocio/CSIC/University of Seville, Avda. Manuel Siurot s/n, 41013 Seville, SpainDepartment of Genetics, University of Seville and Andalusian Center for Molecular Biology and Regenerative Medicine-CABIMER, 41092 Seville, SpainDepartment of Genetics, University of Seville and Andalusian Center for Molecular Biology and Regenerative Medicine-CABIMER, 41092 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, c/Prof. García González nº 1, 41012 Seville, SpainGene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction α was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between K<sub>app</sub> and α was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide’s condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy.https://www.mdpi.com/1999-4923/12/5/482non-toxic nanocarriersspecificity by cancer cellsgene therapytransfection processmetallo-liposomesruthenium(II)-based lipids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José Antonio Lebrón Francisco José Ostos Manuel López-López María Luisa Moyá Carlos Sales Encarnación García Clara Beatriz García-Calderón Margarita García-Calderón María José Peña-Gómez Iván V. Rosado Fernando R. Balestra Pablo Huertas Pilar López-Cornejo |
spellingShingle |
José Antonio Lebrón Francisco José Ostos Manuel López-López María Luisa Moyá Carlos Sales Encarnación García Clara Beatriz García-Calderón Margarita García-Calderón María José Peña-Gómez Iván V. Rosado Fernando R. Balestra Pablo Huertas Pilar López-Cornejo Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material Pharmaceutics non-toxic nanocarriers specificity by cancer cells gene therapy transfection process metallo-liposomes ruthenium(II)-based lipids |
author_facet |
José Antonio Lebrón Francisco José Ostos Manuel López-López María Luisa Moyá Carlos Sales Encarnación García Clara Beatriz García-Calderón Margarita García-Calderón María José Peña-Gómez Iván V. Rosado Fernando R. Balestra Pablo Huertas Pilar López-Cornejo |
author_sort |
José Antonio Lebrón |
title |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
title_short |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
title_full |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
title_fullStr |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
title_full_unstemmed |
Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material |
title_sort |
metallo-liposomes of ruthenium used as promising vectors of genetic material |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2020-05-01 |
description |
Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids derived from the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> complex, RuC11C11 or RuC19C19, both with different hydrophobic/lipophilic ratios, were mixed with the phospholipid DOPE. A relation between the size and the molar fraction α was found and a multidisciplinary study about the interaction between the metallo-liposomes and DNA was performed. The metallo-liposomes/DNA association was quantified and a relationship between K<sub>app</sub> and α was obtained. Techniques such as AFM, SEM, zeta potential, dynamic light scattering and agarose gel electrophoresis demonstrated the formation of lipoplexes and showed the structure of the liposomes. L/D values corresponding to the polynucleotide’s condensation were estimated. In vitro assays proved the low cell toxicity of the metallo-liposomes, lower for normal cells than for cancer cell lines, and a good internalization into cells. The latter as well as the transfection measurements carried out with plasmid DNA pEGFP-C1 have demonstrated a good availability of the Ru(II)-based liposomes for being used as non-toxic nanovectors in gene therapy. |
topic |
non-toxic nanocarriers specificity by cancer cells gene therapy transfection process metallo-liposomes ruthenium(II)-based lipids |
url |
https://www.mdpi.com/1999-4923/12/5/482 |
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