Automated cell identification and tracking using nanoparticle moving-light-displays.
An automated technique for the identification, tracking and analysis of biological cells is presented. It is based on the use of nanoparticles, enclosed within intra-cellular vesicles, to produce clusters of discrete, point-like fluorescent, light sources within the cells. Computational analysis of...
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2012-01-01
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22829889/pdf/?tool=EBI |
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doaj-9eca5d7beb7146dcb8f31eeb9942ddd42021-03-03T20:28:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4083510.1371/journal.pone.0040835Automated cell identification and tracking using nanoparticle moving-light-displays.James A TonkinPaul ReesMartyn R BrownRachel J ErringtonPaul J SmithSally C ChappellHuw D SummersAn automated technique for the identification, tracking and analysis of biological cells is presented. It is based on the use of nanoparticles, enclosed within intra-cellular vesicles, to produce clusters of discrete, point-like fluorescent, light sources within the cells. Computational analysis of these light ensembles in successive time frames of a movie sequence, using k-means clustering and particle tracking algorithms, provides robust and automated discrimination of live cells and their motion and a quantitative measure of their proliferation. This approach is a cytometric version of the moving light display technique which is widely used for analyzing the biological motion of humans and animals. We use the endocytosis of CdTe/ZnS, core-shell quantum dots to produce the light displays within an A549, epithelial, lung cancer cell line, using time-lapse imaging with frame acquisition every 5 minutes over a 40 hour time period. The nanoparticle moving light displays provide simultaneous collection of cell motility data, resolution of mitotic traversal dynamics and identification of familial relationships allowing construction of multi-parameter lineage trees.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22829889/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
James A Tonkin Paul Rees Martyn R Brown Rachel J Errington Paul J Smith Sally C Chappell Huw D Summers |
spellingShingle |
James A Tonkin Paul Rees Martyn R Brown Rachel J Errington Paul J Smith Sally C Chappell Huw D Summers Automated cell identification and tracking using nanoparticle moving-light-displays. PLoS ONE |
author_facet |
James A Tonkin Paul Rees Martyn R Brown Rachel J Errington Paul J Smith Sally C Chappell Huw D Summers |
author_sort |
James A Tonkin |
title |
Automated cell identification and tracking using nanoparticle moving-light-displays. |
title_short |
Automated cell identification and tracking using nanoparticle moving-light-displays. |
title_full |
Automated cell identification and tracking using nanoparticle moving-light-displays. |
title_fullStr |
Automated cell identification and tracking using nanoparticle moving-light-displays. |
title_full_unstemmed |
Automated cell identification and tracking using nanoparticle moving-light-displays. |
title_sort |
automated cell identification and tracking using nanoparticle moving-light-displays. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
An automated technique for the identification, tracking and analysis of biological cells is presented. It is based on the use of nanoparticles, enclosed within intra-cellular vesicles, to produce clusters of discrete, point-like fluorescent, light sources within the cells. Computational analysis of these light ensembles in successive time frames of a movie sequence, using k-means clustering and particle tracking algorithms, provides robust and automated discrimination of live cells and their motion and a quantitative measure of their proliferation. This approach is a cytometric version of the moving light display technique which is widely used for analyzing the biological motion of humans and animals. We use the endocytosis of CdTe/ZnS, core-shell quantum dots to produce the light displays within an A549, epithelial, lung cancer cell line, using time-lapse imaging with frame acquisition every 5 minutes over a 40 hour time period. The nanoparticle moving light displays provide simultaneous collection of cell motility data, resolution of mitotic traversal dynamics and identification of familial relationships allowing construction of multi-parameter lineage trees. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22829889/pdf/?tool=EBI |
work_keys_str_mv |
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