Effects of histatin-1 peptide on human corneal epithelial cells.
Ocular surface and corneal epithelial wounds are common and potentially debilitating problems. Ideal treatments for these injuries would promote epithelial healing without inflammation, infection and scarring. In addition the best treatments would be cost-efficient, effective, non-toxic and easily a...
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doaj-9ece089468e14e569689586a43365e662020-11-25T00:08:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017803010.1371/journal.pone.0178030Effects of histatin-1 peptide on human corneal epithelial cells.Dhara ShahMarwan AliDeepak ShuklaSandeep JainVinay Kumar AakaluOcular surface and corneal epithelial wounds are common and potentially debilitating problems. Ideal treatments for these injuries would promote epithelial healing without inflammation, infection and scarring. In addition the best treatments would be cost-efficient, effective, non-toxic and easily applied. Histatin-1 peptides have been shown to be safe and effective enhancers of epithelial wound healing in other model systems. We sought to determine whether histatin-1 peptides could enhance human corneal epithelial wound healing in vitro.Histatin-1 peptides were applied to human corneal epithelial cells and compared over useful dose ranges in scratch assays using time-lapse microscopy. In addition, path finding analysis, cell spreading assays, toxicity and proliferation assays were performed to further characterize the effects of histatin-1 peptide on human corneal limbal epithelial (HCLE).Histatin-1 enhanced human corneal epithelial wound healing in typical wound healing models. There was minimal toxicity and no significant enhancement of proliferation of corneal epithelium in response to histatin-1 application. Corneal epithelial spreading and pathfinding appeared to be enhanced by the application of histatin-1 peptides.Histatin -1 peptide may enhance migration of HCLE cells and wound healing in vitro. These peptides may have benefit in corneal epithelial wounds and need to be investigated further.http://europepmc.org/articles/PMC5441629?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dhara Shah Marwan Ali Deepak Shukla Sandeep Jain Vinay Kumar Aakalu |
spellingShingle |
Dhara Shah Marwan Ali Deepak Shukla Sandeep Jain Vinay Kumar Aakalu Effects of histatin-1 peptide on human corneal epithelial cells. PLoS ONE |
author_facet |
Dhara Shah Marwan Ali Deepak Shukla Sandeep Jain Vinay Kumar Aakalu |
author_sort |
Dhara Shah |
title |
Effects of histatin-1 peptide on human corneal epithelial cells. |
title_short |
Effects of histatin-1 peptide on human corneal epithelial cells. |
title_full |
Effects of histatin-1 peptide on human corneal epithelial cells. |
title_fullStr |
Effects of histatin-1 peptide on human corneal epithelial cells. |
title_full_unstemmed |
Effects of histatin-1 peptide on human corneal epithelial cells. |
title_sort |
effects of histatin-1 peptide on human corneal epithelial cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Ocular surface and corneal epithelial wounds are common and potentially debilitating problems. Ideal treatments for these injuries would promote epithelial healing without inflammation, infection and scarring. In addition the best treatments would be cost-efficient, effective, non-toxic and easily applied. Histatin-1 peptides have been shown to be safe and effective enhancers of epithelial wound healing in other model systems. We sought to determine whether histatin-1 peptides could enhance human corneal epithelial wound healing in vitro.Histatin-1 peptides were applied to human corneal epithelial cells and compared over useful dose ranges in scratch assays using time-lapse microscopy. In addition, path finding analysis, cell spreading assays, toxicity and proliferation assays were performed to further characterize the effects of histatin-1 peptide on human corneal limbal epithelial (HCLE).Histatin-1 enhanced human corneal epithelial wound healing in typical wound healing models. There was minimal toxicity and no significant enhancement of proliferation of corneal epithelium in response to histatin-1 application. Corneal epithelial spreading and pathfinding appeared to be enhanced by the application of histatin-1 peptides.Histatin -1 peptide may enhance migration of HCLE cells and wound healing in vitro. These peptides may have benefit in corneal epithelial wounds and need to be investigated further. |
url |
http://europepmc.org/articles/PMC5441629?pdf=render |
work_keys_str_mv |
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