Anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy
Abstract Background Antiaris toxicaria has previously shown anticonvulsant activity in acute animal models of epilepsy. The aqueous extract (AAE) was further investigated for activity in kindling with pentylenetetrazole and administration of pilocarpine and kainic acid which mimic temporal lobe epil...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2017-04-01
|
Series: | BMC Research Notes |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13104-017-2488-x |
id |
doaj-9ee88a1b4521497580b7471491b8f659 |
---|---|
record_format |
Article |
spelling |
doaj-9ee88a1b4521497580b7471491b8f6592020-11-25T02:16:18ZengBMCBMC Research Notes1756-05002017-04-011011910.1186/s13104-017-2488-xAnticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsyPriscilla Kolibea Mante0Donatus Wewura Adongo1Eric Woode2Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and TechnologyUniversity of Health and Allied SciencesDepartment of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and TechnologyAbstract Background Antiaris toxicaria has previously shown anticonvulsant activity in acute animal models of epilepsy. The aqueous extract (AAE) was further investigated for activity in kindling with pentylenetetrazole and administration of pilocarpine and kainic acid which mimic temporal lobe epilepsy in various animal species. Results ICR mice and Sprague–Dawley rats were pre-treated with AAE (200–800 mg kg−1) and convulsive episodes induced using pentylenetetrazole, pilocarpine and kainic acid. The potential of AAE to prevent or delay onset and alter duration of seizures were measured. In addition, damage to hippocampal cells was assessed in kainic acid-induced status epilepticus test. 800 mg kg−1 of the extract suppressed the kindled seizure significantly (P < 0.05) as did diazepam. AAE also produced significant effect (P < 0.01) on latency to first myoclonic jerks and on total duration of seizures. The latency to onset of wet dog shakes was increased significantly (P < 0.05) by AAE on kainic acid administration. Carbamazepine and Nifedipine (30 mg kg−1) also delayed the onset. Histopathological examination of brain sections showed no protective effect on hippocampal cells by AAE and nifedipine. Carbamazepine offered better preservation of hippocampal cells in the CA1, CA2 and CA3 regions. Conclusion Antiaris toxicaria may be effective in controlling temporal lobe seizures in rodents.http://link.springer.com/article/10.1186/s13104-017-2488-xHippocampusKainic acidPentylenetetrazolePilocarpineKindling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Priscilla Kolibea Mante Donatus Wewura Adongo Eric Woode |
spellingShingle |
Priscilla Kolibea Mante Donatus Wewura Adongo Eric Woode Anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy BMC Research Notes Hippocampus Kainic acid Pentylenetetrazole Pilocarpine Kindling |
author_facet |
Priscilla Kolibea Mante Donatus Wewura Adongo Eric Woode |
author_sort |
Priscilla Kolibea Mante |
title |
Anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy |
title_short |
Anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy |
title_full |
Anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy |
title_fullStr |
Anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy |
title_full_unstemmed |
Anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy |
title_sort |
anticonvulsant effects of antiaris toxicaria aqueous extract: investigation using animal models of temporal lobe epilepsy |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2017-04-01 |
description |
Abstract Background Antiaris toxicaria has previously shown anticonvulsant activity in acute animal models of epilepsy. The aqueous extract (AAE) was further investigated for activity in kindling with pentylenetetrazole and administration of pilocarpine and kainic acid which mimic temporal lobe epilepsy in various animal species. Results ICR mice and Sprague–Dawley rats were pre-treated with AAE (200–800 mg kg−1) and convulsive episodes induced using pentylenetetrazole, pilocarpine and kainic acid. The potential of AAE to prevent or delay onset and alter duration of seizures were measured. In addition, damage to hippocampal cells was assessed in kainic acid-induced status epilepticus test. 800 mg kg−1 of the extract suppressed the kindled seizure significantly (P < 0.05) as did diazepam. AAE also produced significant effect (P < 0.01) on latency to first myoclonic jerks and on total duration of seizures. The latency to onset of wet dog shakes was increased significantly (P < 0.05) by AAE on kainic acid administration. Carbamazepine and Nifedipine (30 mg kg−1) also delayed the onset. Histopathological examination of brain sections showed no protective effect on hippocampal cells by AAE and nifedipine. Carbamazepine offered better preservation of hippocampal cells in the CA1, CA2 and CA3 regions. Conclusion Antiaris toxicaria may be effective in controlling temporal lobe seizures in rodents. |
topic |
Hippocampus Kainic acid Pentylenetetrazole Pilocarpine Kindling |
url |
http://link.springer.com/article/10.1186/s13104-017-2488-x |
work_keys_str_mv |
AT priscillakolibeamante anticonvulsanteffectsofantiaristoxicariaaqueousextractinvestigationusinganimalmodelsoftemporallobeepilepsy AT donatuswewuraadongo anticonvulsanteffectsofantiaristoxicariaaqueousextractinvestigationusinganimalmodelsoftemporallobeepilepsy AT ericwoode anticonvulsanteffectsofantiaristoxicariaaqueousextractinvestigationusinganimalmodelsoftemporallobeepilepsy |
_version_ |
1724891276043616256 |