Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease Patients
To investigate whether different forms of α-synuclein (α-syn) proteins can induce inflammation and activate the NLRP3 inflammasome, we stimulated with monomeric or aggregated α-syn peripheral blood mononuclear cells of Parkinson disease (PD) patients and age- and sex-matched healthy controls (HC). A...
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doaj-9eecd70b44734144875c4452a472345f2021-03-25T05:16:35ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2021-03-011510.3389/fnins.2021.639646639646Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease PatientsFederica Piancone0Marina Saresella1Francesca La Rosa2Ivana Marventano3Mario Meloni4Jorge Navarro5Mario Clerici6Mario Clerici7Laboratory of Molecular Medicine and Biotechnology, IRCCS Fondazione don C Gnocchi, Milan, ItalyLaboratory of Molecular Medicine and Biotechnology, IRCCS Fondazione don C Gnocchi, Milan, ItalyLaboratory of Molecular Medicine and Biotechnology, IRCCS Fondazione don C Gnocchi, Milan, ItalyLaboratory of Molecular Medicine and Biotechnology, IRCCS Fondazione don C Gnocchi, Milan, ItalyDepartment of Neurology, IRCCS Fondazione don C Gnocchi, Milan, ItalyDepartment of Neurology, IRCCS Fondazione don C Gnocchi, Milan, ItalyLaboratory of Molecular Medicine and Biotechnology, IRCCS Fondazione don C Gnocchi, Milan, ItalyDepartment of Physiopathology and Transplants, University of Milano, Milan, ItalyTo investigate whether different forms of α-synuclein (α-syn) proteins can induce inflammation and activate the NLRP3 inflammasome, we stimulated with monomeric or aggregated α-syn peripheral blood mononuclear cells of Parkinson disease (PD) patients and age- and sex-matched healthy controls (HC). ASC-speck formation, i.e., the intracellular generation of functionally active inflammasome complexes, as well as the production of inflammasome-related [caspase-1, interleukin 1β (IL-18), and IL-1β], and pro–IL-6, or anti–IL-10 inflammatory cytokines were evaluated. Gastrointestinal permeability, suggested to be altered in PD, was also investigated by measuring plasma concentration of lipopolysaccharide (LPS) and I-FABP (fatty acid–binding protein). ASC-speck expression, as well as IL-18 and caspase-1 production and LPS and I-FABP plasma concentration, was comparable in PD and HC, indicating that α-syn does not stimulate the NLRP3 inflammasome and that PD does not associate with alterations of intestinal permeability. Interestingly, though, IL-1β and IL-6 production was increased, whereas that of IL-10 was reduced in α-syn–stimulated cells of PD compared to HC, suggesting that PD-associated neuroinflammation is not the consequence of the activation of the NLRP3 inflammasome but rather of an imbalance between proinflammatory and anti-inflammatory cytokines.https://www.frontiersin.org/articles/10.3389/fnins.2021.639646/fullParkinson’s diseaseα-synucleininflammationinflammasomecytokines |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Federica Piancone Marina Saresella Francesca La Rosa Ivana Marventano Mario Meloni Jorge Navarro Mario Clerici Mario Clerici |
spellingShingle |
Federica Piancone Marina Saresella Francesca La Rosa Ivana Marventano Mario Meloni Jorge Navarro Mario Clerici Mario Clerici Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease Patients Frontiers in Neuroscience Parkinson’s disease α-synuclein inflammation inflammasome cytokines |
author_facet |
Federica Piancone Marina Saresella Francesca La Rosa Ivana Marventano Mario Meloni Jorge Navarro Mario Clerici Mario Clerici |
author_sort |
Federica Piancone |
title |
Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease Patients |
title_short |
Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease Patients |
title_full |
Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease Patients |
title_fullStr |
Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease Patients |
title_full_unstemmed |
Inflammatory Responses to Monomeric and Aggregated α-Synuclein in Peripheral Blood of Parkinson Disease Patients |
title_sort |
inflammatory responses to monomeric and aggregated α-synuclein in peripheral blood of parkinson disease patients |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroscience |
issn |
1662-453X |
publishDate |
2021-03-01 |
description |
To investigate whether different forms of α-synuclein (α-syn) proteins can induce inflammation and activate the NLRP3 inflammasome, we stimulated with monomeric or aggregated α-syn peripheral blood mononuclear cells of Parkinson disease (PD) patients and age- and sex-matched healthy controls (HC). ASC-speck formation, i.e., the intracellular generation of functionally active inflammasome complexes, as well as the production of inflammasome-related [caspase-1, interleukin 1β (IL-18), and IL-1β], and pro–IL-6, or anti–IL-10 inflammatory cytokines were evaluated. Gastrointestinal permeability, suggested to be altered in PD, was also investigated by measuring plasma concentration of lipopolysaccharide (LPS) and I-FABP (fatty acid–binding protein). ASC-speck expression, as well as IL-18 and caspase-1 production and LPS and I-FABP plasma concentration, was comparable in PD and HC, indicating that α-syn does not stimulate the NLRP3 inflammasome and that PD does not associate with alterations of intestinal permeability. Interestingly, though, IL-1β and IL-6 production was increased, whereas that of IL-10 was reduced in α-syn–stimulated cells of PD compared to HC, suggesting that PD-associated neuroinflammation is not the consequence of the activation of the NLRP3 inflammasome but rather of an imbalance between proinflammatory and anti-inflammatory cytokines. |
topic |
Parkinson’s disease α-synuclein inflammation inflammasome cytokines |
url |
https://www.frontiersin.org/articles/10.3389/fnins.2021.639646/full |
work_keys_str_mv |
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