Production of unstable proteins through the formation of stable core complexes

Flexible or disordered domains often hinder the purification of proteins involved in functional interactions. Here the authors describe an approach that enables the production of stable and functional complexes of otherwise unstable proteins in quantities sufficient for structural and functional stu...

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Main Authors:	Nicolas Levy, Sylvia Eiler, Karine Pradeau-Aubreton, Benoit Maillot, François Stricher, Marc Ruff
Format:	Article
Language:	English
Published:	Nature Publishing Group 2016-03-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms10932

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spelling	doaj-9ef95dc3bce44f668e21a106a000cf312021-05-11T11:09:52ZengNature Publishing GroupNature Communications2041-17232016-03-01711910.1038/ncomms10932Production of unstable proteins through the formation of stable core complexesNicolas Levy0Sylvia Eiler1Karine Pradeau-Aubreton2Benoit Maillot3François Stricher4Marc Ruff5IGBMC, UDS, CNRS, INSERMIGBMC, UDS, CNRS, INSERMIGBMC, UDS, CNRS, INSERMIGBMC, UDS, CNRS, INSERMIGBMC, UDS, CNRS, INSERMIGBMC, UDS, CNRS, INSERMFlexible or disordered domains often hinder the purification of proteins involved in functional interactions. Here the authors describe an approach that enables the production of stable and functional complexes of otherwise unstable proteins in quantities sufficient for structural and functional studies.https://doi.org/10.1038/ncomms10932
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Nicolas Levy Sylvia Eiler Karine Pradeau-Aubreton Benoit Maillot François Stricher Marc Ruff
spellingShingle	Nicolas Levy Sylvia Eiler Karine Pradeau-Aubreton Benoit Maillot François Stricher Marc Ruff Production of unstable proteins through the formation of stable core complexes Nature Communications
author_facet	Nicolas Levy Sylvia Eiler Karine Pradeau-Aubreton Benoit Maillot François Stricher Marc Ruff
author_sort	Nicolas Levy
title	Production of unstable proteins through the formation of stable core complexes
title_short	Production of unstable proteins through the formation of stable core complexes
title_full	Production of unstable proteins through the formation of stable core complexes
title_fullStr	Production of unstable proteins through the formation of stable core complexes
title_full_unstemmed	Production of unstable proteins through the formation of stable core complexes
title_sort	production of unstable proteins through the formation of stable core complexes
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2016-03-01
description	Flexible or disordered domains often hinder the purification of proteins involved in functional interactions. Here the authors describe an approach that enables the production of stable and functional complexes of otherwise unstable proteins in quantities sufficient for structural and functional studies.
url	https://doi.org/10.1038/ncomms10932
work_keys_str_mv	AT nicolaslevy productionofunstableproteinsthroughtheformationofstablecorecomplexes AT sylviaeiler productionofunstableproteinsthroughtheformationofstablecorecomplexes AT karinepradeauaubreton productionofunstableproteinsthroughtheformationofstablecorecomplexes AT benoitmaillot productionofunstableproteinsthroughtheformationofstablecorecomplexes AT francoisstricher productionofunstableproteinsthroughtheformationofstablecorecomplexes AT marcruff productionofunstableproteinsthroughtheformationofstablecorecomplexes
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Production of unstable proteins through the formation of stable core complexes

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