Methodological Establishment of HPLC-DAD for Determination of 10 Active Ingredients in Wuweizi

• Main Authors: Feng YAO, Da-shuai CHEN
• Format: Article
• Language: English
• Published: Third Party Medicine International Publishing Group Co. Limited 2015-09-01
• Series: Journal of International Translational Medicine
• Subjects: High performance liquid chromatography-diode array detection; Wuweizi; Lignan; Microwave-assisted extraction; Concentration determination
• Online Access: http://www.jitm.hk/EN/abstract/abstract159.shtml

Objective: To establish high performance liquid chromatography-diode array detection (HPLC-DAD) method for the determination of active ingredients of multiple lignans in Nanwuweizi (Kadsura longepedunculata) and Beiwuweizi (Fructus Schisandrae Chinensis) so as to set up the standard for the quality control of Wuweizi (Schisandra chinensis). Methods: Brava BDS C18 column (250 mm×4.6 mm, 5 μm) was applied, with detection condition as follows: column temperature: 30℃; mobile phase: water (A)-acetonitrile (B); gradient elution: 0.8 mL/min and detection wavelength: 225 nm.Results: Ten ingredients in Beiwuweizi and 5 in Nanwuweizi were completely separately within 75 min. Peak area was in favorable linear relationship with concentration. In addition, the recovery rate was 96.87-103.29% and relative standard deviation (RSD) was 0.68-2.09%.Conclusion: HPLC-DAD is simple and reliable with favorable repeatability, so it can be used for the determination of multiple lignans in Wuweizi.