Role of membrane compartment occupied by Can1 (MCC) and eisosome subdomains in plant pathogenicity of the necrotrophic fungus Alternaria brassicicola

• Main Authors: Justine Colou, Guillaume Quang N’Guyen, Ophélie Dubreu, Kévin Fontaine, Anthony Kwasiborski, Franck Bastide, Florence Manero, Bruno Hamon, Sophie Aligon, Philippe Simoneau, Thomas Guillemette
• Format: Article
• Language: English
• Published: BMC 2019-12-01
• Series: BMC Microbiology
• Subjects: Eisosome; Fungi; Plant pathogen; Appressoria; Seed; Plasma membrane
• Online Access: https://doi.org/10.1186/s12866-019-1667-4

Abstract Background MCC/eisosomes are membrane microdomains that have been proposed to participate in the plasma membrane function in particular by regulating the homeostasis of lipids, promoting the recruitment of specific proteins and acting as provider of membrane reservoirs. Results Here we showed that several potential MCC/eisosomal protein encoding genes in the necrotrophic fungus A. brassicicola were overexpressed when germinated spores were exposed to antimicrobial defence compounds, osmotic and hydric stresses, which are major constraints encountered by the fungus during the plant colonization process. Mutants deficient for key MCC/eisosome components did not exhibit any enhanced susceptibility to phytoalexins and to applied stress conditions compared to the reference strain, except for a slight hypersensitivity of the ∆∆abpil1a-abpil1b strain to 2 M sorbitol. Depending on the considered mutants, we showed that the leaf and silique colonization processes were impaired by comparison to the wild-type, and assumed that these defects in aggressiveness were probably caused by a reduced appressorium formation rate. Conclusions This is the first study on the role of MCC/eisosomes in the pathogenic process of a plant pathogenic fungus. A link between these membrane domains and the fungus ability to form functional penetration structures was shown, providing new potential directions for plant disease control strategies.