Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti Children
Caveolin-1 (CAV1) variants have been suggested to be associated with obesity and related metabolic disorders, but information based on human studies is limited. In the present study, we aimed to investigate the potential association between the CAV1 rs1997623 C/A variant and metabolic syndrome (MetS...
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Frontiers Media S.A.
2018-12-01
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Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00689/full |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rasheeba Nizam Ebaa Al-Ozairi Jo Max Goodson Motesam Melhem Lena Davidsson Hessa Alkhandari Ashraf Al Madhoun Sara Shamsah Malak Qaddoumi Ghazi Alghanim Nouf Alhasawi Mohamed Abu-Farha Jehad Abubaker Ping Shi Mor-Li Hartman Mary Tavares Milad Bitar Hamad Ali Hossein Arefanian Sriraman Devarajan Faisal Al-Refaei Osama Alsmadi Jaakko Tuomilehto Fahd Al-Mulla |
spellingShingle |
Rasheeba Nizam Ebaa Al-Ozairi Jo Max Goodson Motesam Melhem Lena Davidsson Hessa Alkhandari Ashraf Al Madhoun Sara Shamsah Malak Qaddoumi Ghazi Alghanim Nouf Alhasawi Mohamed Abu-Farha Jehad Abubaker Ping Shi Mor-Li Hartman Mary Tavares Milad Bitar Hamad Ali Hossein Arefanian Sriraman Devarajan Faisal Al-Refaei Osama Alsmadi Jaakko Tuomilehto Fahd Al-Mulla Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti Children Frontiers in Genetics CAV1 HDLC metabolic syndrome Kuwaiti children obesity |
author_facet |
Rasheeba Nizam Ebaa Al-Ozairi Jo Max Goodson Motesam Melhem Lena Davidsson Hessa Alkhandari Ashraf Al Madhoun Sara Shamsah Malak Qaddoumi Ghazi Alghanim Nouf Alhasawi Mohamed Abu-Farha Jehad Abubaker Ping Shi Mor-Li Hartman Mary Tavares Milad Bitar Hamad Ali Hossein Arefanian Sriraman Devarajan Faisal Al-Refaei Osama Alsmadi Jaakko Tuomilehto Fahd Al-Mulla |
author_sort |
Rasheeba Nizam |
title |
Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti Children |
title_short |
Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti Children |
title_full |
Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti Children |
title_fullStr |
Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti Children |
title_full_unstemmed |
Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti Children |
title_sort |
caveolin-1 variant is associated with the metabolic syndrome in kuwaiti children |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2018-12-01 |
description |
Caveolin-1 (CAV1) variants have been suggested to be associated with obesity and related metabolic disorders, but information based on human studies is limited. In the present study, we aimed to investigate the potential association between the CAV1 rs1997623 C/A variant and metabolic syndrome (MetS) in Kuwaiti children. DNA from saliva samples collected from 1313 Kuwaiti children (mean age: 12 years) were genotyped using the TaqMan SNP genotyping assay. The classification of MetS was based on the presence/absence of four indicators; (1) central obesity, (2) elevated systolic or diastolic blood pressure, (3) low salivary high-density lipoprotein cholesterol (HDLC), and (4) high salivary glucose. In this study, children with MetS scored ≥3, children in the intermediate metabolic group scored 1 or 2 and children without MetS scored 0. About one-third of the children were obese. A total of 246 children (18.7%) were classified as having MetS; 834 children (63.5%) were in the intermediate metabolic group, and 233 children (17.7%) had no indication of MetS. Obesity was highly prevalent in the MetS group (91.9%) while 26.8% of children were obese in the intermediate metabolic group. None of the children were obese in the group without MetS. Analysis of the CAV1 rs1997623 variant revealed a significant association of the A-allele (p = 0.01, Odds Ratio (OR) = 1.66) and the heterozygous CA-genotype (p = 0.005, OR = 1.88) with MetS. Consistently, the A-allele (p = 0.002, OR = 1.71) and CA-genotype (p = 0.005, OR = 1.70) also showed significant association with the intermediate metabolic group. Furthermore, the A-allele (p = 0.01, OR = 1.33) and the CA-genotype (p = 0.008, OR = 1.55) were associated with low levels of saliva HDLC. Individuals who were heterozygous or homozygous for the variant (CA/AA) showed significantly lower levels of high HDLC compared to those harboring the CC-genotype (p = 0.023). Our study revealed a novel association of the CAV1 rs1997623 variant with the MetS and with low saliva HDLC levels in young Kuwaiti children and indicated the need for further in-depth studies to unravel the role of CAV1 gene in the genetic etiology of MetS. |
topic |
CAV1 HDLC metabolic syndrome Kuwaiti children obesity |
url |
https://www.frontiersin.org/article/10.3389/fgene.2018.00689/full |
work_keys_str_mv |
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doaj-9f2c18cab3db4466894fb8fcf7a07d862020-11-25T02:16:40ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-12-01910.3389/fgene.2018.00689398923Caveolin-1 Variant Is Associated With the Metabolic Syndrome in Kuwaiti ChildrenRasheeba Nizam0Ebaa Al-Ozairi1Jo Max Goodson2Motesam Melhem3Lena Davidsson4Hessa Alkhandari5Ashraf Al Madhoun6Sara Shamsah7Malak Qaddoumi8Ghazi Alghanim9Nouf Alhasawi10Mohamed Abu-Farha11Jehad Abubaker12Ping Shi13Mor-Li Hartman14Mary Tavares15Milad Bitar16Hamad Ali17Hossein Arefanian18Sriraman Devarajan19Faisal Al-Refaei20Osama Alsmadi21Jaakko Tuomilehto22Fahd Al-Mulla23Functional Genomics Unit, Dasman Diabetes Institute, Kuwait City, KuwaitClinical Division, Dasman Diabetes Institute, Kuwait City, KuwaitApplied Oral Sciences, The Forsyth Institute, Cambridge, MA, United StatesFunctional Genomics Unit, Dasman Diabetes Institute, Kuwait City, KuwaitFamily Medicine and Pediatric Unit, Dasman Diabetes Institute, Kuwait City, KuwaitFamily Medicine and Pediatric Unit, Dasman Diabetes Institute, Kuwait City, KuwaitFunctional Genomics Unit, Dasman Diabetes Institute, Kuwait City, KuwaitFaculty of Allied Health Sciences, Kuwait University, Kuwait City, KuwaitFunctional Genomics Unit, Dasman Diabetes Institute, Kuwait City, KuwaitFunctional Genomics Unit, Dasman Diabetes Institute, Kuwait City, KuwaitFunctional Genomics Unit, Dasman Diabetes Institute, Kuwait City, KuwaitBiochemistry and Molecular Biology Unit, Dasman Diabetes Institute, Kuwait City, KuwaitBiochemistry and Molecular Biology Unit, Dasman Diabetes Institute, Kuwait City, KuwaitApplied Oral Sciences, The Forsyth Institute, Cambridge, MA, United StatesApplied Oral Sciences, The Forsyth Institute, Cambridge, MA, United StatesApplied Oral Sciences, The Forsyth Institute, Cambridge, MA, United StatesFaculty of Medicine, Kuwait University, Kuwait City, KuwaitFaculty of Allied Health Sciences, Kuwait University, Kuwait City, KuwaitImmunology Unit, Dasman Diabetes Institute, Kuwait City, KuwaitNational Dasman Diabetes Biobank, Dasman Diabetes Institute, Kuwait City, KuwaitClinical Division, Dasman Diabetes Institute, Kuwait City, Kuwait0Cell Therapy and Applied Genomics, King Hussein Cancer Center, Amman, Jordan1Research Division, Dasman Diabetes Institute, Kuwait City, KuwaitFunctional Genomics Unit, Dasman Diabetes Institute, Kuwait City, KuwaitCaveolin-1 (CAV1) variants have been suggested to be associated with obesity and related metabolic disorders, but information based on human studies is limited. In the present study, we aimed to investigate the potential association between the CAV1 rs1997623 C/A variant and metabolic syndrome (MetS) in Kuwaiti children. DNA from saliva samples collected from 1313 Kuwaiti children (mean age: 12 years) were genotyped using the TaqMan SNP genotyping assay. The classification of MetS was based on the presence/absence of four indicators; (1) central obesity, (2) elevated systolic or diastolic blood pressure, (3) low salivary high-density lipoprotein cholesterol (HDLC), and (4) high salivary glucose. In this study, children with MetS scored ≥3, children in the intermediate metabolic group scored 1 or 2 and children without MetS scored 0. About one-third of the children were obese. A total of 246 children (18.7%) were classified as having MetS; 834 children (63.5%) were in the intermediate metabolic group, and 233 children (17.7%) had no indication of MetS. Obesity was highly prevalent in the MetS group (91.9%) while 26.8% of children were obese in the intermediate metabolic group. None of the children were obese in the group without MetS. Analysis of the CAV1 rs1997623 variant revealed a significant association of the A-allele (p = 0.01, Odds Ratio (OR) = 1.66) and the heterozygous CA-genotype (p = 0.005, OR = 1.88) with MetS. Consistently, the A-allele (p = 0.002, OR = 1.71) and CA-genotype (p = 0.005, OR = 1.70) also showed significant association with the intermediate metabolic group. Furthermore, the A-allele (p = 0.01, OR = 1.33) and the CA-genotype (p = 0.008, OR = 1.55) were associated with low levels of saliva HDLC. Individuals who were heterozygous or homozygous for the variant (CA/AA) showed significantly lower levels of high HDLC compared to those harboring the CC-genotype (p = 0.023). Our study revealed a novel association of the CAV1 rs1997623 variant with the MetS and with low saliva HDLC levels in young Kuwaiti children and indicated the need for further in-depth studies to unravel the role of CAV1 gene in the genetic etiology of MetS.https://www.frontiersin.org/article/10.3389/fgene.2018.00689/fullCAV1HDLCmetabolic syndromeKuwaiti childrenobesity |