Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil
The objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observ...
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Associação Brasileira de Divulgação Científica
2012-05-01
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doaj-9f736c22cf4b4f54b35c058b6b9ae3522020-11-24T22:20:53ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2012-05-01455436443Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oilC.G. HeldweinL.L. SilvaP. ReckziegelF.M.C. BarrosM.E. BürgerB. BaldisserottoC.A. MallmannD. SchmidtB.O. CaronB.M. HeinzmannThe objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observe the induction and potentiation of the anesthetic effect of EO, juvenile silver catfish (9.30 ± 1.85 g; 10.15 ± 0.95 cm; N = 6) were exposed to various concentrations of L. alba EO in the presence or absence of diazepam [an agonist of high-affinity binding sites for benzodiazepinic (BDZ) sites coupled to the GABA A receptor complex]. In another experiment, fish (N = 6) were initially anesthetized with the EO and then transferred to an anesthetic-free aquarium containing flumazenil (a selective antagonist of binding sites for BDZ coupled to the GABA A receptor complex) or water to assess recovery time from the anesthesia. In this case, flumazenil was used to observe the involvement of the GABA-BDZ receptor in the EO mechanism of action. The results showed that diazepam potentiates the anesthetic effect of EO at all concentrations tested. Fish exposed to diazepam and EO showed faster recovery from anesthesia when flumazenil was added to the recovery bath (12.0 ± 0.3 and 7.2 ± 0.7, respectively) than those exposed to water (9.2 ± 0.2 and 3.5 ± 0.3, respectively). In conclusion, the results demonstrated the involvement of the GABAergic system in the anesthetic effect of L. alba EO on silver catfish.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500009False-melissaSilver catfishBenzodiazepinesFlumazenilGABA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
C.G. Heldwein L.L. Silva P. Reckziegel F.M.C. Barros M.E. Bürger B. Baldisserotto C.A. Mallmann D. Schmidt B.O. Caron B.M. Heinzmann |
spellingShingle |
C.G. Heldwein L.L. Silva P. Reckziegel F.M.C. Barros M.E. Bürger B. Baldisserotto C.A. Mallmann D. Schmidt B.O. Caron B.M. Heinzmann Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil Brazilian Journal of Medical and Biological Research False-melissa Silver catfish Benzodiazepines Flumazenil GABA |
author_facet |
C.G. Heldwein L.L. Silva P. Reckziegel F.M.C. Barros M.E. Bürger B. Baldisserotto C.A. Mallmann D. Schmidt B.O. Caron B.M. Heinzmann |
author_sort |
C.G. Heldwein |
title |
Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil |
title_short |
Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil |
title_full |
Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil |
title_fullStr |
Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil |
title_full_unstemmed |
Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil |
title_sort |
participation of the gabaergic system in the anesthetic effect of lippia alba (mill.) n.e. brown essential oil |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2012-05-01 |
description |
The objective of this study was to identify the possible involvement of the GABAergic system in the anesthetic effect of Lippia alba essential oil (EO). We propose a new animal model using silver catfish (Rhamdia quelen) exposed to an anesthetic bath to study the mechanism of action of EO. To observe the induction and potentiation of the anesthetic effect of EO, juvenile silver catfish (9.30 ± 1.85 g; 10.15 ± 0.95 cm; N = 6) were exposed to various concentrations of L. alba EO in the presence or absence of diazepam [an agonist of high-affinity binding sites for benzodiazepinic (BDZ) sites coupled to the GABA A receptor complex]. In another experiment, fish (N = 6) were initially anesthetized with the EO and then transferred to an anesthetic-free aquarium containing flumazenil (a selective antagonist of binding sites for BDZ coupled to the GABA A receptor complex) or water to assess recovery time from the anesthesia. In this case, flumazenil was used to observe the involvement of the GABA-BDZ receptor in the EO mechanism of action. The results showed that diazepam potentiates the anesthetic effect of EO at all concentrations tested. Fish exposed to diazepam and EO showed faster recovery from anesthesia when flumazenil was added to the recovery bath (12.0 ± 0.3 and 7.2 ± 0.7, respectively) than those exposed to water (9.2 ± 0.2 and 3.5 ± 0.3, respectively). In conclusion, the results demonstrated the involvement of the GABAergic system in the anesthetic effect of L. alba EO on silver catfish. |
topic |
False-melissa Silver catfish Benzodiazepines Flumazenil GABA |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500009 |
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