Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent
Two chromium resistant alkalophilic bacterial strains (CRAB), namely Bacillus cohnii SR2 and Bacillus licheniformis SR3 were isolated and identified from tannery effluent. Bioreduction of chromium (VI) into chromium (III) by CRAB was investigated in the present study. Optimization of process paramet...
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doaj-9fa75a0e2209485380e863dbb0752d9b2020-11-25T03:05:53ZengElsevierJournal of King Saud University: Science1018-36472020-04-0132319691977Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluentRaja Kumaresan Sarankumar0Arumugam Arulprakash1Sandhanasamy Devanesan2Adikesavan Selvi3Mohamad S. AlSalhi4Aruliah Rajasekar5Anis Ahamed6Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, IndiaEnvironmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, IndiaDepartment of Physics and Astronomy, College of Science, King Saud University, P. Box: 2455, Riyadh 11451, Saudi ArabiaEnvironmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, IndiaDepartment of Physics and Astronomy, College of Science, King Saud University, P. Box: 2455, Riyadh 11451, Saudi Arabia; Corresponding authors.Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, India; Corresponding authors.Department of Botany and Microbiology, College of Science, King Saud University, P. Box: 2455, Riyadh 11451, Saudi ArabiaTwo chromium resistant alkalophilic bacterial strains (CRAB), namely Bacillus cohnii SR2 and Bacillus licheniformis SR3 were isolated and identified from tannery effluent. Bioreduction of chromium (VI) into chromium (III) by CRAB was investigated in the present study. Optimization of process parameters with respect to bioreduction percentage was studied by Response Surface Methodology (RSM) software, v12. Both the strains were found to achieve highest chromium (VI) bioreduction of 94 and 95% in 100 mg/L at the end of 25 h at pH 9.0. At an optimized concentration of 550 mg/L, a maximum bioreduction of 82 and 90% by SR2 and SR3 was noted within 25 h. In addition, both the strains showed tolerance to chromium until 1000 mg/L. Scale-up studies using Air lift bioreactor system by CRAB with real tannery effluent showed 100% bioreduction within 8 h. Therefore, this study signified the astonishing bioreduction capability of CRAB isolates that was quite evident in scale-up studies too. Keywords: Alkalophilic, Bioreduction, Chromium resistant, Response surface methodology, Tannery effluenthttp://www.sciencedirect.com/science/article/pii/S1018364720300598 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raja Kumaresan Sarankumar Arumugam Arulprakash Sandhanasamy Devanesan Adikesavan Selvi Mohamad S. AlSalhi Aruliah Rajasekar Anis Ahamed |
spellingShingle |
Raja Kumaresan Sarankumar Arumugam Arulprakash Sandhanasamy Devanesan Adikesavan Selvi Mohamad S. AlSalhi Aruliah Rajasekar Anis Ahamed Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent Journal of King Saud University: Science |
author_facet |
Raja Kumaresan Sarankumar Arumugam Arulprakash Sandhanasamy Devanesan Adikesavan Selvi Mohamad S. AlSalhi Aruliah Rajasekar Anis Ahamed |
author_sort |
Raja Kumaresan Sarankumar |
title |
Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent |
title_short |
Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent |
title_full |
Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent |
title_fullStr |
Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent |
title_full_unstemmed |
Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent |
title_sort |
bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent |
publisher |
Elsevier |
series |
Journal of King Saud University: Science |
issn |
1018-3647 |
publishDate |
2020-04-01 |
description |
Two chromium resistant alkalophilic bacterial strains (CRAB), namely Bacillus cohnii SR2 and Bacillus licheniformis SR3 were isolated and identified from tannery effluent. Bioreduction of chromium (VI) into chromium (III) by CRAB was investigated in the present study. Optimization of process parameters with respect to bioreduction percentage was studied by Response Surface Methodology (RSM) software, v12. Both the strains were found to achieve highest chromium (VI) bioreduction of 94 and 95% in 100 mg/L at the end of 25 h at pH 9.0. At an optimized concentration of 550 mg/L, a maximum bioreduction of 82 and 90% by SR2 and SR3 was noted within 25 h. In addition, both the strains showed tolerance to chromium until 1000 mg/L. Scale-up studies using Air lift bioreactor system by CRAB with real tannery effluent showed 100% bioreduction within 8 h. Therefore, this study signified the astonishing bioreduction capability of CRAB isolates that was quite evident in scale-up studies too. Keywords: Alkalophilic, Bioreduction, Chromium resistant, Response surface methodology, Tannery effluent |
url |
http://www.sciencedirect.com/science/article/pii/S1018364720300598 |
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