Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation
The change in the gut microbiome and microbial metabolites in a patient suffering from severe and enduring anorexia nervosa (AN) and diagnosed with small intestinal bacterial overgrowth syndrome (SIBO) was investigated. Microbial gut dysbiosis is associated with both AN and SIBO, and therefore gut m...
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Format: | Article |
Language: | English |
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MDPI AG
2019-09-01
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Series: | Microorganisms |
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Online Access: | https://www.mdpi.com/2076-2607/7/9/338 |
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doaj-9fdb62c220d440748d376f6af1566838 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Petra Prochazkova Radka Roubalova Jiri Dvorak Helena Tlaskalova-Hogenova Martina Cermakova Petra Tomasova Blanka Sediva Marek Kuzma Josef Bulant Martin Bilej Pavel Hrabak Eva Meisnerova Alena Lambertova Hana Papezova |
spellingShingle |
Petra Prochazkova Radka Roubalova Jiri Dvorak Helena Tlaskalova-Hogenova Martina Cermakova Petra Tomasova Blanka Sediva Marek Kuzma Josef Bulant Martin Bilej Pavel Hrabak Eva Meisnerova Alena Lambertova Hana Papezova Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation Microorganisms microbiome microbial metabolites fecal microbiota transplantation (FMT) small intestinal bacterial overgrowth syndrome short-chain fatty acids Firmicutes/Bacteroides <i>Akkermansia</i> |
author_facet |
Petra Prochazkova Radka Roubalova Jiri Dvorak Helena Tlaskalova-Hogenova Martina Cermakova Petra Tomasova Blanka Sediva Marek Kuzma Josef Bulant Martin Bilej Pavel Hrabak Eva Meisnerova Alena Lambertova Hana Papezova |
author_sort |
Petra Prochazkova |
title |
Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation |
title_short |
Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation |
title_full |
Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation |
title_fullStr |
Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation |
title_full_unstemmed |
Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation |
title_sort |
microbiota, microbial metabolites, and barrier function in a patient with anorexia nervosa after fecal microbiota transplantation |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2019-09-01 |
description |
The change in the gut microbiome and microbial metabolites in a patient suffering from severe and enduring anorexia nervosa (AN) and diagnosed with small intestinal bacterial overgrowth syndrome (SIBO) was investigated. Microbial gut dysbiosis is associated with both AN and SIBO, and therefore gut microbiome changes by serial fecal microbiota transplantation (FMT) is a possible therapeutic modality. This study assessed the effects of FMT on gut barrier function, microbiota composition, and the levels of bacterial metabolic products. The patient treatment with FMT led to the improvement of gut barrier function, which was altered prior to FMT. Very low bacterial alpha diversity, a lack of beneficial bacteria, together with a great abundance of fungal species were observed in the patient stool sample before FMT. After FMT, both bacterial species richness and gut microbiome evenness increased in the patient, while the fungal alpha diversity decreased. The total short-chain fatty acids (SCFAs) levels (molecules presenting an important source of energy for epithelial gut cells) gradually increased after FMT. Contrarily, one of the most abundant intestinal neurotransmitters, serotonin, tended to decrease throughout the observation period. Overall, gut microbial dysbiosis improvement after FMT was considered. However, there were no signs of patient clinical improvement. The need for an in-depth analysis of the donor´s stool and correct selection pre-FMT is evident. |
topic |
microbiome microbial metabolites fecal microbiota transplantation (FMT) small intestinal bacterial overgrowth syndrome short-chain fatty acids Firmicutes/Bacteroides <i>Akkermansia</i> |
url |
https://www.mdpi.com/2076-2607/7/9/338 |
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doaj-9fdb62c220d440748d376f6af15668382020-11-25T01:51:11ZengMDPI AGMicroorganisms2076-26072019-09-017933810.3390/microorganisms7090338microorganisms7090338Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota TransplantationPetra Prochazkova0Radka Roubalova1Jiri Dvorak2Helena Tlaskalova-Hogenova3Martina Cermakova4Petra Tomasova5Blanka Sediva6Marek Kuzma7Josef Bulant8Martin Bilej9Pavel Hrabak10Eva Meisnerova11Alena Lambertova12Hana Papezova13Laboratory of Cellular and Molecular Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicLaboratory of Cellular and Molecular Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicLaboratory of Cellular and Molecular Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicLaboratory of Cellular and Molecular Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicLaboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicLaboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicLaboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicLaboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 121 08 Prague 2, Czech RepublicLaboratory of Cellular and Molecular Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, 142 20 Prague 4, Czech RepublicDepartment of Internal Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 121 08 Prague 2, Czech RepublicDepartment of Internal Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 121 08 Prague 2, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 121 08 Prague 2, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 121 08 Prague 2, Czech RepublicThe change in the gut microbiome and microbial metabolites in a patient suffering from severe and enduring anorexia nervosa (AN) and diagnosed with small intestinal bacterial overgrowth syndrome (SIBO) was investigated. Microbial gut dysbiosis is associated with both AN and SIBO, and therefore gut microbiome changes by serial fecal microbiota transplantation (FMT) is a possible therapeutic modality. This study assessed the effects of FMT on gut barrier function, microbiota composition, and the levels of bacterial metabolic products. The patient treatment with FMT led to the improvement of gut barrier function, which was altered prior to FMT. Very low bacterial alpha diversity, a lack of beneficial bacteria, together with a great abundance of fungal species were observed in the patient stool sample before FMT. After FMT, both bacterial species richness and gut microbiome evenness increased in the patient, while the fungal alpha diversity decreased. The total short-chain fatty acids (SCFAs) levels (molecules presenting an important source of energy for epithelial gut cells) gradually increased after FMT. Contrarily, one of the most abundant intestinal neurotransmitters, serotonin, tended to decrease throughout the observation period. Overall, gut microbial dysbiosis improvement after FMT was considered. However, there were no signs of patient clinical improvement. The need for an in-depth analysis of the donor´s stool and correct selection pre-FMT is evident.https://www.mdpi.com/2076-2607/7/9/338microbiomemicrobial metabolitesfecal microbiota transplantation (FMT)small intestinal bacterial overgrowth syndromeshort-chain fatty acidsFirmicutes/Bacteroides<i>Akkermansia</i> |