Anti-tubercular activity of a natural stilbene and its synthetic derivatives
Objectives: Tuberculosis (TB) and multidrug- and extensively drug-resistant TB in particular are remaining a major global health challenge and efficient new drugs against TB are needed. This study evaluated the anti-tubercular activity of a natural stilbene and its synthetic derivatives against .Met...
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German Medical Science GMS Publishing House
2018-02-01
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doaj-9ff9143b47944e0283a4ea1a23a3fe972020-11-25T02:21:00ZengGerman Medical Science GMS Publishing HouseGMS Infectious Diseases2195-88312018-02-016Doc0110.3205/id000036Anti-tubercular activity of a natural stilbene and its synthetic derivativesReinheimer, Claudia0Büttner, Dominik1Proschak, Eugen2Bode, Helge B.3Kempf, Volkhard A. J.4Wichelhaus, Thomas A.5Institute of Medical Microbiology and Infection Control, Hospital of Goethe-University, Frankfurt, GermanyInstitute of Pharmaceutical Chemistry, Goethe-University, Frankfurt, GermanyInstitute of Pharmaceutical Chemistry, Goethe-University, Frankfurt, GermanyMerck endowed chair for Molecular Biotechnology, Department of Biosciences and Buchmann Institute for Molecular Life Sciences (BMLS), Goethe-University, Frankfurt, GermanyInstitute of Medical Microbiology and Infection Control, Hospital of Goethe-University, Frankfurt, GermanyInstitute of Medical Microbiology and Infection Control, Hospital of Goethe-University, Frankfurt, GermanyObjectives: Tuberculosis (TB) and multidrug- and extensively drug-resistant TB in particular are remaining a major global health challenge and efficient new drugs against TB are needed. This study evaluated the anti-tubercular activity of a natural stilbene and its synthetic derivatives against .Methods: Isopropylstilbene and its synthetic derivatives were analyzed for their anti-tubercular activity against ATCC 27294 as well as multidrug- and extensively drug-resistant clinical isolates by using MGIT 960 instrumentation and EpiCenter software equipped with TB eXiST module. Cytotoxic effects of drug candidates were determined by a MTT dye reduction assay using A549 adenocarcinomic human alveolar basal epithelial cells.Results: Growth of ATCC 27294 was suppressed by the natural isopropylstilbene HB64 as well as synthetic derivatives DB56 and DB55 at 25 µg/ml. Growth of clinical isolates MDR and XDR was suppressed by HB64 at 100 µg/ml as well as by synthetic derivatives DB56 and DB55 at 50 µg/ml and 25 µg/ml, respectively. No anti-tubercular activity was demonstrated for synthetic derivatives DB53, EB251, and RB57 at 100 µg/ml. Toxicity in terms of IC values of HB64, DB55 and DB56 were 7.92 µg/ml, 12.15 µg/ml and 16.01 µg/ml, respectively.Conclusions: Synthetical derivatives of stilbene might be effective candidates as anti-tubercular drugs. However, toxicity of these substances as determined by IC values might limit therapeutic success . Further investigations should address lowering the toxicity for parenteral administration by remodeling stilbene derivatives.http://www.egms.de/static/en/journals/id/2018-6/id000036.shtmltuberculosisdrug resistancenew substancesepoxide hydrolasesstilbene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reinheimer, Claudia Büttner, Dominik Proschak, Eugen Bode, Helge B. Kempf, Volkhard A. J. Wichelhaus, Thomas A. |
spellingShingle |
Reinheimer, Claudia Büttner, Dominik Proschak, Eugen Bode, Helge B. Kempf, Volkhard A. J. Wichelhaus, Thomas A. Anti-tubercular activity of a natural stilbene and its synthetic derivatives GMS Infectious Diseases tuberculosis drug resistance new substances epoxide hydrolases stilbene |
author_facet |
Reinheimer, Claudia Büttner, Dominik Proschak, Eugen Bode, Helge B. Kempf, Volkhard A. J. Wichelhaus, Thomas A. |
author_sort |
Reinheimer, Claudia |
title |
Anti-tubercular activity of a natural stilbene and its synthetic derivatives |
title_short |
Anti-tubercular activity of a natural stilbene and its synthetic derivatives |
title_full |
Anti-tubercular activity of a natural stilbene and its synthetic derivatives |
title_fullStr |
Anti-tubercular activity of a natural stilbene and its synthetic derivatives |
title_full_unstemmed |
Anti-tubercular activity of a natural stilbene and its synthetic derivatives |
title_sort |
anti-tubercular activity of a natural stilbene and its synthetic derivatives |
publisher |
German Medical Science GMS Publishing House |
series |
GMS Infectious Diseases |
issn |
2195-8831 |
publishDate |
2018-02-01 |
description |
Objectives: Tuberculosis (TB) and multidrug- and extensively drug-resistant TB in particular are remaining a major global health challenge and efficient new drugs against TB are needed. This study evaluated the anti-tubercular activity of a natural stilbene and its synthetic derivatives against .Methods: Isopropylstilbene and its synthetic derivatives were analyzed for their anti-tubercular activity against ATCC 27294 as well as multidrug- and extensively drug-resistant clinical isolates by using MGIT 960 instrumentation and EpiCenter software equipped with TB eXiST module. Cytotoxic effects of drug candidates were determined by a MTT dye reduction assay using A549 adenocarcinomic human alveolar basal epithelial cells.Results: Growth of ATCC 27294 was suppressed by the natural isopropylstilbene HB64 as well as synthetic derivatives DB56 and DB55 at 25 µg/ml. Growth of clinical isolates MDR and XDR was suppressed by HB64 at 100 µg/ml as well as by synthetic derivatives DB56 and DB55 at 50 µg/ml and 25 µg/ml, respectively. No anti-tubercular activity was demonstrated for synthetic derivatives DB53, EB251, and RB57 at 100 µg/ml. Toxicity in terms of IC values of HB64, DB55 and DB56 were 7.92 µg/ml, 12.15 µg/ml and 16.01 µg/ml, respectively.Conclusions: Synthetical derivatives of stilbene might be effective candidates as anti-tubercular drugs. However, toxicity of these substances as determined by IC values might limit therapeutic success . Further investigations should address lowering the toxicity for parenteral administration by remodeling stilbene derivatives. |
topic |
tuberculosis drug resistance new substances epoxide hydrolases stilbene |
url |
http://www.egms.de/static/en/journals/id/2018-6/id000036.shtml |
work_keys_str_mv |
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