PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
PVP-capped silver nanoparticles with a diameter of the metallic core of 70 nm, a hydrodynamic diameter of 120 nm and a zeta potential of −20 mV were prepared and investigated with regard to their biological activity. This review summarizes the physicochemical properties (dissolution, protein adsorpt...
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Format: | Article |
Language: | English |
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Beilstein-Institut
2014-11-01
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Series: | Beilstein Journal of Nanotechnology |
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Online Access: | https://doi.org/10.3762/bjnano.5.205 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sebastian Ahlberg Alexandra Antonopulos Jörg Diendorf Ralf Dringen Matthias Epple Rebekka Flöck Wolfgang Goedecke Christina Graf Nadine Haberl Jens Helmlinger Fabian Herzog Frederike Heuer Stephanie Hirn Christian Johannes Stefanie Kittler Manfred Köller Katrin Korn Wolfgang G. Kreyling Fritz Krombach Jürgen Lademann Kateryna Loza Eva M. Luther Marcelina Malissek Martina C. Meinke Daniel Nordmeyer Anne Pailliart Jörg Raabe Fiorenza Rancan Barbara Rothen-Rutishauser Eckart Rühl Carsten Schleh Andreas Seibel Christina Sengstock Lennart Treuel Annika Vogt Katrin Weber Reinhard Zellner |
spellingShingle |
Sebastian Ahlberg Alexandra Antonopulos Jörg Diendorf Ralf Dringen Matthias Epple Rebekka Flöck Wolfgang Goedecke Christina Graf Nadine Haberl Jens Helmlinger Fabian Herzog Frederike Heuer Stephanie Hirn Christian Johannes Stefanie Kittler Manfred Köller Katrin Korn Wolfgang G. Kreyling Fritz Krombach Jürgen Lademann Kateryna Loza Eva M. Luther Marcelina Malissek Martina C. Meinke Daniel Nordmeyer Anne Pailliart Jörg Raabe Fiorenza Rancan Barbara Rothen-Rutishauser Eckart Rühl Carsten Schleh Andreas Seibel Christina Sengstock Lennart Treuel Annika Vogt Katrin Weber Reinhard Zellner PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments Beilstein Journal of Nanotechnology aerosols biological properties cell biology nanoparticles nanotoxicology silver |
author_facet |
Sebastian Ahlberg Alexandra Antonopulos Jörg Diendorf Ralf Dringen Matthias Epple Rebekka Flöck Wolfgang Goedecke Christina Graf Nadine Haberl Jens Helmlinger Fabian Herzog Frederike Heuer Stephanie Hirn Christian Johannes Stefanie Kittler Manfred Köller Katrin Korn Wolfgang G. Kreyling Fritz Krombach Jürgen Lademann Kateryna Loza Eva M. Luther Marcelina Malissek Martina C. Meinke Daniel Nordmeyer Anne Pailliart Jörg Raabe Fiorenza Rancan Barbara Rothen-Rutishauser Eckart Rühl Carsten Schleh Andreas Seibel Christina Sengstock Lennart Treuel Annika Vogt Katrin Weber Reinhard Zellner |
author_sort |
Sebastian Ahlberg |
title |
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments |
title_short |
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments |
title_full |
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments |
title_fullStr |
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments |
title_full_unstemmed |
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments |
title_sort |
pvp-coated, negatively charged silver nanoparticles: a multi-center study of their physicochemical characteristics, cell culture and in vivo experiments |
publisher |
Beilstein-Institut |
series |
Beilstein Journal of Nanotechnology |
issn |
2190-4286 |
publishDate |
2014-11-01 |
description |
PVP-capped silver nanoparticles with a diameter of the metallic core of 70 nm, a hydrodynamic diameter of 120 nm and a zeta potential of −20 mV were prepared and investigated with regard to their biological activity. This review summarizes the physicochemical properties (dissolution, protein adsorption, dispersability) of these nanoparticles and the cellular consequences of the exposure of a broad range of biological test systems to this defined type of silver nanoparticles. Silver nanoparticles dissolve in water in the presence of oxygen. In addition, in biological media (i.e., in the presence of proteins) the surface of silver nanoparticles is rapidly coated by a protein corona that influences their physicochemical and biological properties including cellular uptake. Silver nanoparticles are taken up by cell-type specific endocytosis pathways as demonstrated for hMSC, primary T-cells, primary monocytes, and astrocytes. A visualization of particles inside cells is possible by X-ray microscopy, fluorescence microscopy, and combined FIB/SEM analysis. By staining organelles, their localization inside the cell can be additionally determined. While primary brain astrocytes are shown to be fairly tolerant toward silver nanoparticles, silver nanoparticles induce the formation of DNA double-strand-breaks (DSB) and lead to chromosomal aberrations and sister-chromatid exchanges in Chinese hamster fibroblast cell lines (CHO9, K1, V79B). An exposure of rats to silver nanoparticles in vivo induced a moderate pulmonary toxicity, however, only at rather high concentrations. The same was found in precision-cut lung slices of rats in which silver nanoparticles remained mainly at the tissue surface. In a human 3D triple-cell culture model consisting of three cell types (alveolar epithelial cells, macrophages, and dendritic cells), adverse effects were also only found at high silver concentrations. The silver ions that are released from silver nanoparticles may be harmful to skin with disrupted barrier (e.g., wounds) and induce oxidative stress in skin cells (HaCaT). In conclusion, the data obtained on the effects of this well-defined type of silver nanoparticles on various biological systems clearly demonstrate that cell-type specific properties as well as experimental conditions determine the biocompatibility of and the cellular responses to an exposure with silver nanoparticles. |
topic |
aerosols biological properties cell biology nanoparticles nanotoxicology silver |
url |
https://doi.org/10.3762/bjnano.5.205 |
work_keys_str_mv |
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doaj-9ff9b4c808fe4650a9603784c6b227612020-11-24T22:01:55ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862014-11-01511944196510.3762/bjnano.5.2052190-4286-5-205PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experimentsSebastian Ahlberg0Alexandra Antonopulos1Jörg Diendorf2Ralf Dringen3Matthias Epple4Rebekka Flöck5Wolfgang Goedecke6Christina Graf7Nadine Haberl8Jens Helmlinger9Fabian Herzog10Frederike Heuer11Stephanie Hirn12Christian Johannes13Stefanie Kittler14Manfred Köller15Katrin Korn16Wolfgang G. Kreyling17Fritz Krombach18Jürgen Lademann19Kateryna Loza20Eva M. Luther21Marcelina Malissek22Martina C. Meinke23Daniel Nordmeyer24Anne Pailliart25Jörg Raabe26Fiorenza Rancan27Barbara Rothen-Rutishauser28Eckart Rühl29Carsten Schleh30Andreas Seibel31Christina Sengstock32Lennart Treuel33Annika Vogt34Katrin Weber35Reinhard Zellner36See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.See end of main text.PVP-capped silver nanoparticles with a diameter of the metallic core of 70 nm, a hydrodynamic diameter of 120 nm and a zeta potential of −20 mV were prepared and investigated with regard to their biological activity. This review summarizes the physicochemical properties (dissolution, protein adsorption, dispersability) of these nanoparticles and the cellular consequences of the exposure of a broad range of biological test systems to this defined type of silver nanoparticles. Silver nanoparticles dissolve in water in the presence of oxygen. In addition, in biological media (i.e., in the presence of proteins) the surface of silver nanoparticles is rapidly coated by a protein corona that influences their physicochemical and biological properties including cellular uptake. Silver nanoparticles are taken up by cell-type specific endocytosis pathways as demonstrated for hMSC, primary T-cells, primary monocytes, and astrocytes. A visualization of particles inside cells is possible by X-ray microscopy, fluorescence microscopy, and combined FIB/SEM analysis. By staining organelles, their localization inside the cell can be additionally determined. While primary brain astrocytes are shown to be fairly tolerant toward silver nanoparticles, silver nanoparticles induce the formation of DNA double-strand-breaks (DSB) and lead to chromosomal aberrations and sister-chromatid exchanges in Chinese hamster fibroblast cell lines (CHO9, K1, V79B). An exposure of rats to silver nanoparticles in vivo induced a moderate pulmonary toxicity, however, only at rather high concentrations. The same was found in precision-cut lung slices of rats in which silver nanoparticles remained mainly at the tissue surface. In a human 3D triple-cell culture model consisting of three cell types (alveolar epithelial cells, macrophages, and dendritic cells), adverse effects were also only found at high silver concentrations. The silver ions that are released from silver nanoparticles may be harmful to skin with disrupted barrier (e.g., wounds) and induce oxidative stress in skin cells (HaCaT). In conclusion, the data obtained on the effects of this well-defined type of silver nanoparticles on various biological systems clearly demonstrate that cell-type specific properties as well as experimental conditions determine the biocompatibility of and the cellular responses to an exposure with silver nanoparticles.https://doi.org/10.3762/bjnano.5.205aerosolsbiological propertiescell biologynanoparticlesnanotoxicologysilver |