Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells
In this study, we mainly aimed at developing the Ifosfamide-loaded-lipid-core nanocapsules (IFS-LNC) to increase the therapeutic efficacy in osteosarcoma. The nanoparticle was prepared and evaluated in terms of physical, chemical and biological parameters. The lipid-core-nanocapsules were nanosized...
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doaj-9ffe97ed3a9d49ddb519c1ace92181952020-11-24T22:26:33ZengElsevierSaudi Journal of Biological Sciences1319-562X2018-09-0125611401145Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cellsSheng-Qun Wang0Qiao Zhang1Chao Sun2Guang-Yao Liu3Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, ChinaDepartment of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, ChinaDepartment of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, ChinaCorresponding author at: Department of Orthopaedics, China-Japan Union Hospital of Jilin University, 176 Xiantai Street, Changchun, Jilin 130033, China. Fax: +86 431 89876922.; Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, ChinaIn this study, we mainly aimed at developing the Ifosfamide-loaded-lipid-core nanocapsules (IFS-LNC) to increase the therapeutic efficacy in osteosarcoma. The nanoparticle was prepared and evaluated in terms of physical, chemical and biological parameters. The lipid-core-nanocapsules were nanosized with narrow particle size distribution and exhibited a high loading capacity. The LNC displayed a sustained release profile of the drug suggesting its potential application in biomedical field and prolonged anticancer therapy. The LNC showed an endocytosis-mediated cellular uptake in MG63 cancer cells which may lead to an accelerated disruption of the acidic endolysosomal vesicles with release of IFS into the cytoplasm. Specifically, IFS-LNC exhibited a significantly higher cytotoxicity than free IFS used at the same concentration. The indiscriminate ability of the drug-loaded formulation increased the apoptosis of cancer cells by increasing the expression levels of caspase-3 and caspase-9 in MG63 cells. Overall, nanoparticulate formulations of Ifosfamide enhanced the therapeutic efficacy in osteosarcoma. Keywords: Osteosarcoma, Nanocapsules, Ifosfamide, Apoptosis, Chemotherapyhttp://www.sciencedirect.com/science/article/pii/S1319562X16301772 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sheng-Qun Wang Qiao Zhang Chao Sun Guang-Yao Liu |
spellingShingle |
Sheng-Qun Wang Qiao Zhang Chao Sun Guang-Yao Liu Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells Saudi Journal of Biological Sciences |
author_facet |
Sheng-Qun Wang Qiao Zhang Chao Sun Guang-Yao Liu |
author_sort |
Sheng-Qun Wang |
title |
Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells |
title_short |
Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells |
title_full |
Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells |
title_fullStr |
Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells |
title_full_unstemmed |
Ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in MG63 osteosarcoma cells |
title_sort |
ifosfamide-loaded lipid-core-nanocapsules to increase the anticancer efficacy in mg63 osteosarcoma cells |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2018-09-01 |
description |
In this study, we mainly aimed at developing the Ifosfamide-loaded-lipid-core nanocapsules (IFS-LNC) to increase the therapeutic efficacy in osteosarcoma. The nanoparticle was prepared and evaluated in terms of physical, chemical and biological parameters. The lipid-core-nanocapsules were nanosized with narrow particle size distribution and exhibited a high loading capacity. The LNC displayed a sustained release profile of the drug suggesting its potential application in biomedical field and prolonged anticancer therapy. The LNC showed an endocytosis-mediated cellular uptake in MG63 cancer cells which may lead to an accelerated disruption of the acidic endolysosomal vesicles with release of IFS into the cytoplasm. Specifically, IFS-LNC exhibited a significantly higher cytotoxicity than free IFS used at the same concentration. The indiscriminate ability of the drug-loaded formulation increased the apoptosis of cancer cells by increasing the expression levels of caspase-3 and caspase-9 in MG63 cells. Overall, nanoparticulate formulations of Ifosfamide enhanced the therapeutic efficacy in osteosarcoma. Keywords: Osteosarcoma, Nanocapsules, Ifosfamide, Apoptosis, Chemotherapy |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X16301772 |
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