Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia
Background: In early 2019, an outbreak of severe dengue was reported in Manado, North Sulawesi Province, Indonesia. This epidemic raised public concern and recorded the highest number of cases in the last 10 years. This study aimed to determine the clinical spectrum, disease aetiology and virologica...
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doaj-a0046ce33cda4d72a217fbe4a1cae7b32021-05-22T04:35:54ZengElsevierInternational Journal of Infectious Diseases1201-97122021-05-01106185196Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, IndonesiaSuryadi N.N. Tatura0Dionisius Denis1Marsha S. Santoso2Rahma F. Hayati3Billy J. Kepel4Benediktus Yohan5R. Tedjo Sasmono6Division of Paediatric Infection and Tropical Medicine, Department of Paediatrics, Faculty of Medicine, Sam Ratulangi University, and Prof. R.D. Kandou General Hospital, Manado, North Sulawesi, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaFaculty of Medicine, Sam Ratulangi University, Manado, North Sulawesi, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaEijkman Institute for Molecular Biology, Jakarta, Indonesia; Corresponding author at: Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta 10430, Indonesia.Background: In early 2019, an outbreak of severe dengue was reported in Manado, North Sulawesi Province, Indonesia. This epidemic raised public concern and recorded the highest number of cases in the last 10 years. This study aimed to determine the clinical spectrum, disease aetiology and virological characteristics associated with this outbreak of severe dengue. Methods: Dengue was diagnosed using non-structural protein 1 detection, reverse transcription polymerase chain reaction and immunoglobulin (Ig)G/IgM serology. Envelope gene sequencing was conducted to determine the phylogeny of the dengue virus (DENV). Results: In total, 146 patients with a median age of 8 years (interquartile range IQR 5–11 years) were recruited. Most patients experienced expanded dengue syndrome, characterized by severe organ involvement including liver enlargement, stomach ache and coagulation problems. During the outbreak, DENV-3 was the dominant serotype (75.9%). Smaller numbers of DENV-1, -2 and -4 were also detected. Phylogenetically, the dominant DENV-3 strains were grouped in multiple clusters and were related to other Indonesian strains, suggesting the emergence of heterogenous local viruses. Conclusion: The occurrence of an outbreak of severe dengue in Manado was confirmed, and DENV-3 was found to be the dominant serotype during the outbreak. This study shows the benefits of virological surveillance in understanding the aetiological agents responsible for outbreaks of severe dengue.http://www.sciencedirect.com/science/article/pii/S1201971221002836DengueOutbreakSevereSerotypeGenotype |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Suryadi N.N. Tatura Dionisius Denis Marsha S. Santoso Rahma F. Hayati Billy J. Kepel Benediktus Yohan R. Tedjo Sasmono |
spellingShingle |
Suryadi N.N. Tatura Dionisius Denis Marsha S. Santoso Rahma F. Hayati Billy J. Kepel Benediktus Yohan R. Tedjo Sasmono Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia International Journal of Infectious Diseases Dengue Outbreak Severe Serotype Genotype |
author_facet |
Suryadi N.N. Tatura Dionisius Denis Marsha S. Santoso Rahma F. Hayati Billy J. Kepel Benediktus Yohan R. Tedjo Sasmono |
author_sort |
Suryadi N.N. Tatura |
title |
Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia |
title_short |
Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia |
title_full |
Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia |
title_fullStr |
Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia |
title_full_unstemmed |
Outbreak of severe dengue associated with DENV-3 in the city of Manado, North Sulawesi, Indonesia |
title_sort |
outbreak of severe dengue associated with denv-3 in the city of manado, north sulawesi, indonesia |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 |
publishDate |
2021-05-01 |
description |
Background: In early 2019, an outbreak of severe dengue was reported in Manado, North Sulawesi Province, Indonesia. This epidemic raised public concern and recorded the highest number of cases in the last 10 years. This study aimed to determine the clinical spectrum, disease aetiology and virological characteristics associated with this outbreak of severe dengue. Methods: Dengue was diagnosed using non-structural protein 1 detection, reverse transcription polymerase chain reaction and immunoglobulin (Ig)G/IgM serology. Envelope gene sequencing was conducted to determine the phylogeny of the dengue virus (DENV). Results: In total, 146 patients with a median age of 8 years (interquartile range IQR 5–11 years) were recruited. Most patients experienced expanded dengue syndrome, characterized by severe organ involvement including liver enlargement, stomach ache and coagulation problems. During the outbreak, DENV-3 was the dominant serotype (75.9%). Smaller numbers of DENV-1, -2 and -4 were also detected. Phylogenetically, the dominant DENV-3 strains were grouped in multiple clusters and were related to other Indonesian strains, suggesting the emergence of heterogenous local viruses. Conclusion: The occurrence of an outbreak of severe dengue in Manado was confirmed, and DENV-3 was found to be the dominant serotype during the outbreak. This study shows the benefits of virological surveillance in understanding the aetiological agents responsible for outbreaks of severe dengue. |
topic |
Dengue Outbreak Severe Serotype Genotype |
url |
http://www.sciencedirect.com/science/article/pii/S1201971221002836 |
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