Yeast Sensors for Novel Drugs: Chloroquine and Others Revealed

• Main Authors: Elizabeth Coetsee, Hendrik Swart, Pieter van Wyk, Carolina Pohl, Khumisho Dithebe, Andries Olivier, Chantel Swart, Lodewyk Kock
• Format: Article
• Language: English
• Published: MDPI AG 2012-09-01
• Series: Sensors
• Subjects: anticancer; anti-malarial; anti-mitochondrial; chloroquine; Lipomyces; NanoSAM; preservation; pro-mitochondrial; sensors; yeasts
• Online Access: http://www.mdpi.com/1424-8220/12/10/13058

In this study the mitochondrion is regarded as a target to reveal compounds that may be used to combat various diseases. Consequently, the sexual structures of yeasts (with high mitochondrial activity) were identified as sensors to screen for various anti-mitochondrial drugs that may be toxic to humans and that are directed, amongst others, against fungal diseases and cancer. Strikingly, these sensors indicated that chloroquine is a potent pro-mitochondrial drug which stimulated yeast sexual reproduction. In addition, these sensors   also showed that some Non-Steroidal Anti-Inflammatory drugs (NSAIDs), anti-malarial drugs, antifungal and anticancer drugs are anti-mitochondrial. These yeast sensor bio-assays may fast track studies aimed at discovering new drugs as well as their mechanisms and should now be further evaluated for selectivity towards anti-/ pro-mitochondrials, fertility drugs and contraceptives, using in vitro, in vivo, in silico and omics research.