Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i>
Echinoderms, such as the rock-boring sea urchin <i>Paracentrotus lividus</i>, attach temporarily to surfaces during locomotion using their tube feet. They can attach firmly to any substrate and release from it within seconds through the secretion of unknown molecules. The composition of...
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doaj-a02171efaabf44aea1346c7fa9ed77132020-11-25T02:03:24ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121394610.3390/ijms21030946ijms21030946Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i>Robert Pjeta0Herbert Lindner1Leopold Kremser2Willi Salvenmoser3Daniel Sobral4Peter Ladurner5Romana Santos6Institute of Zoology and Center of Molecular Biosciences Innsbruck, University of Innsbruck, 6020 Innsbruck, AustriaDivision of Clinical Biochemistry, Biocenter, Innsbruck Medical University, 6020 Innsbruck, AustriaDivision of Clinical Biochemistry, Biocenter, Innsbruck Medical University, 6020 Innsbruck, AustriaInstitute of Zoology and Center of Molecular Biosciences Innsbruck, University of Innsbruck, 6020 Innsbruck, AustriaDepartamento Ciências da Vida, Faculdade de Ciências e Tecnologia–Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, PortugalInstitute of Zoology and Center of Molecular Biosciences Innsbruck, University of Innsbruck, 6020 Innsbruck, AustriaCentro de Ciências do Mar e do Ambiente, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, PortugalEchinoderms, such as the rock-boring sea urchin <i>Paracentrotus lividus</i>, attach temporarily to surfaces during locomotion using their tube feet. They can attach firmly to any substrate and release from it within seconds through the secretion of unknown molecules. The composition of the adhesive, as well as the releasing secretion, remains largely unknown. This study re-analyzed a differential proteome dataset from Lebesgue et al. by mapping mass spectrometry-derived peptides to a <i>P. lividus</i> <i>de novo</i> transcriptome generated in this study. This resulted in a drastic increase in mapped proteins in comparison to the previous publication. The data were subsequently combined with a differential RNAseq approach to identify potential adhesion candidate genes. A gene expression analysis of 59 transcripts using whole mount in situ hybridization led to the identification of 16 transcripts potentially involved in bioadhesion. In the future these data could be useful for the production of synthetic reversible adhesives for industrial and medical purposes.https://www.mdpi.com/1422-0067/21/3/946echinodermsea urchinbioadhesiondifferential rnaseqmass spectrometryin situ hybridization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Robert Pjeta Herbert Lindner Leopold Kremser Willi Salvenmoser Daniel Sobral Peter Ladurner Romana Santos |
spellingShingle |
Robert Pjeta Herbert Lindner Leopold Kremser Willi Salvenmoser Daniel Sobral Peter Ladurner Romana Santos Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i> International Journal of Molecular Sciences echinoderm sea urchin bioadhesion differential rnaseq mass spectrometry in situ hybridization |
author_facet |
Robert Pjeta Herbert Lindner Leopold Kremser Willi Salvenmoser Daniel Sobral Peter Ladurner Romana Santos |
author_sort |
Robert Pjeta |
title |
Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i> |
title_short |
Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i> |
title_full |
Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i> |
title_fullStr |
Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i> |
title_full_unstemmed |
Integrative Transcriptome and Proteome Analysis of the Tube Foot and Adhesive Secretions of the Sea Urchin <i>Paracentrotus lividus</i> |
title_sort |
integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin <i>paracentrotus lividus</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
Echinoderms, such as the rock-boring sea urchin <i>Paracentrotus lividus</i>, attach temporarily to surfaces during locomotion using their tube feet. They can attach firmly to any substrate and release from it within seconds through the secretion of unknown molecules. The composition of the adhesive, as well as the releasing secretion, remains largely unknown. This study re-analyzed a differential proteome dataset from Lebesgue et al. by mapping mass spectrometry-derived peptides to a <i>P. lividus</i> <i>de novo</i> transcriptome generated in this study. This resulted in a drastic increase in mapped proteins in comparison to the previous publication. The data were subsequently combined with a differential RNAseq approach to identify potential adhesion candidate genes. A gene expression analysis of 59 transcripts using whole mount in situ hybridization led to the identification of 16 transcripts potentially involved in bioadhesion. In the future these data could be useful for the production of synthetic reversible adhesives for industrial and medical purposes. |
topic |
echinoderm sea urchin bioadhesion differential rnaseq mass spectrometry in situ hybridization |
url |
https://www.mdpi.com/1422-0067/21/3/946 |
work_keys_str_mv |
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