Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in Romania
Cereals are very susceptible to fungal attacks. Fungi have a unique biochemical pathway to assimilate a vast array of available substrates and produce toxic secondary metabolites, such as mycotoxins, which represent a clear public health concern. In this context, a maize survey was conducted in orde...
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doaj-a053e6b0fbcf4a0b8bbb499cf9af577c2020-12-02T06:40:43ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Agriculture1843-52461843-53862020-11-01772425210.15835/buasvmcn-agr:2020.001511359Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in RomaniaIrina SMEU0Elena Mirela CUCU1Alina Alexandra DOBRE2Hellene CASIAN3National R&D Institute for Food Bioresources - IBA Bucharest, Microbiology-ELISA Laboratory, 6th Dinu Vintilă Street, 2nd District, 020323 BucharestNational R&D Institute for Food Bioresources - IBA Bucharest, Microbiology-ELISA Laboratory, 6th Dinu Vintilă Street, 2nd District, 020323 BucharestNational R&D Institute for Food Bioresources - IBA Bucharest, Microbiology-ELISA Laboratory, 6th Dinu Vintilă Street, 2nd District, 020323 BucharestUniversity of Agronomic Sciences and Veterinary Medicine of Bucharest, 59th Mărăști Boulevard, 1st District, 011464 BucharestCereals are very susceptible to fungal attacks. Fungi have a unique biochemical pathway to assimilate a vast array of available substrates and produce toxic secondary metabolites, such as mycotoxins, which represent a clear public health concern. In this context, a maize survey was conducted in order to assess the diversity of mycotoxin-producing fungi. Low levels of total aflatoxins, acceptable by the European Union, were detected in maize samples. A semi-automated Biolog® Microbial Identification System was used for the identification of the fungal strains. Enzyme-linked immunosorbent assay (ELISA) was used for the quantification of total aflatoxins. The results indicated that Fusarium udum and Rhizopus oryzae were the prevalent fungi for the assessed maize samples, while both control and treated samples showed low levels of total aflatoxins, which did not exceed 1.5 μg kg-1. The registered total aflatoxin concentrations were consistent with the European regulations.https://journals.usamvcluj.ro/index.php/agriculture/article/view/13922aflatoxinsbiolog® systemmaize. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Irina SMEU Elena Mirela CUCU Alina Alexandra DOBRE Hellene CASIAN |
spellingShingle |
Irina SMEU Elena Mirela CUCU Alina Alexandra DOBRE Hellene CASIAN Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in Romania Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Agriculture aflatoxins biolog® system maize. |
author_facet |
Irina SMEU Elena Mirela CUCU Alina Alexandra DOBRE Hellene CASIAN |
author_sort |
Irina SMEU |
title |
Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in Romania |
title_short |
Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in Romania |
title_full |
Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in Romania |
title_fullStr |
Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in Romania |
title_full_unstemmed |
Application of the Biolog® Identification System for Aflatoxin-Producing Fungi Associated with Maize (Zea mays L.) Contamination in Romania |
title_sort |
application of the biolog® identification system for aflatoxin-producing fungi associated with maize (zea mays l.) contamination in romania |
publisher |
AcademicPres |
series |
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Agriculture |
issn |
1843-5246 1843-5386 |
publishDate |
2020-11-01 |
description |
Cereals are very susceptible to fungal attacks. Fungi have a unique biochemical pathway to assimilate a vast array of available substrates and produce toxic secondary metabolites, such as mycotoxins, which represent a clear public health concern. In this context, a maize survey was conducted in order to assess the diversity of mycotoxin-producing fungi. Low levels of total aflatoxins, acceptable by the European Union, were detected in maize samples. A semi-automated Biolog® Microbial Identification System was used for the identification of the fungal strains. Enzyme-linked immunosorbent assay (ELISA) was used for the quantification of total aflatoxins. The results indicated that Fusarium udum and Rhizopus oryzae were the prevalent fungi for the assessed maize samples, while both control and treated samples showed low levels of total aflatoxins, which did not exceed 1.5 μg kg-1. The registered total aflatoxin concentrations were consistent with the European regulations. |
topic |
aflatoxins biolog® system maize. |
url |
https://journals.usamvcluj.ro/index.php/agriculture/article/view/13922 |
work_keys_str_mv |
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