Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

• Main Authors: Bin Zhao, Xinhui Pei, Hualing Fu, Heidi Qunhui Xie, Wenjing Tian
• Format: Article
• Language: English
• Published: MDPI AG 2012-12-01
• Series: Sensors
• Subjects: ELISA; dioxins; Ah-I; multi-analyte immunoassay
• Online Access: http://www.mdpi.com/1424-8220/12/12/16710

With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.