Applicability of papain solutions in immunohematology

• Main Authors: Laís Priscila De Santis, Patrícia Carvalho Garcia, Valéria Nogueira Dias Paes Secco, Rosana Rossi Ferreira, Elenice Deffune
• Format: Article
• Language: English
• Published: Instituto Israelita de Ensino e Pesquisa Albert Einstein
• Series: Einstein (São Paulo)
• Subjects: Antígenos; Alergia e imunologia; Papaína; Enzimas
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082019000200208&lng=en&tlng=en

ABSTRACT Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.