Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19

The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute...

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Main Authors: Huey-Ling You, Meng-Chih Lin, Chen-Hsiang Lee
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:Biomedical Journal
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2319417020302353
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spelling doaj-a0ac88f66a4047cc8df7e5f7e8c3aa102021-04-28T06:09:13ZengElsevierBiomedical Journal2319-41702021-03-01441101104Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19Huey-Ling You0Meng-Chih Lin1Chen-Hsiang Lee2Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; Department of Medical Laboratory Sciences and Biotechnology, Fooyin University, Kaohsiung, TaiwanDivision of Chest Medicine, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; College of Medicine, Chang Gung University, Taoyuan, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan; Corresponding author. Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, 123, Ta-Pei Rd., Naio-Sung, Kaohsiung 833, Taiwan.The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and is considered to be the gold standard. In this report, we share our experience of using two different testing platforms: the cobas 6800 SARS-CoV-2 test, an automated system that was recently granted Emergency Use Authorization by the FDA, and a laboratory-developed test based on the protocol from the Taiwan Centers for Disease Control (CDC). There was an overall 96.2% agreement between the two platforms. However, the positive agreement between the two platforms was only 80.0%. We found 3 instances of discordance between the two systems and this emphasized the need for timely diagnosis with a reliable testing platform.http://www.sciencedirect.com/science/article/pii/S2319417020302353CoronavirusMolecular diagnosticsViral loadRT-PCRRNA extraction
collection DOAJ
language English
format Article
sources DOAJ
author Huey-Ling You
Meng-Chih Lin
Chen-Hsiang Lee
spellingShingle Huey-Ling You
Meng-Chih Lin
Chen-Hsiang Lee
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
Biomedical Journal
Coronavirus
Molecular diagnostics
Viral load
RT-PCR
RNA extraction
author_facet Huey-Ling You
Meng-Chih Lin
Chen-Hsiang Lee
author_sort Huey-Ling You
title Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
title_short Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
title_full Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
title_fullStr Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
title_full_unstemmed Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
title_sort comparison of the roche cobas 6800 sars-cov-2 test and the taiwan cdc protocol for the molecular diagnosis of covid-19
publisher Elsevier
series Biomedical Journal
issn 2319-4170
publishDate 2021-03-01
description The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and is considered to be the gold standard. In this report, we share our experience of using two different testing platforms: the cobas 6800 SARS-CoV-2 test, an automated system that was recently granted Emergency Use Authorization by the FDA, and a laboratory-developed test based on the protocol from the Taiwan Centers for Disease Control (CDC). There was an overall 96.2% agreement between the two platforms. However, the positive agreement between the two platforms was only 80.0%. We found 3 instances of discordance between the two systems and this emphasized the need for timely diagnosis with a reliable testing platform.
topic Coronavirus
Molecular diagnostics
Viral load
RT-PCR
RNA extraction
url http://www.sciencedirect.com/science/article/pii/S2319417020302353
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