Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute...
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doaj-a0ac88f66a4047cc8df7e5f7e8c3aa102021-04-28T06:09:13ZengElsevierBiomedical Journal2319-41702021-03-01441101104Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19Huey-Ling You0Meng-Chih Lin1Chen-Hsiang Lee2Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; Department of Medical Laboratory Sciences and Biotechnology, Fooyin University, Kaohsiung, TaiwanDivision of Chest Medicine, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; College of Medicine, Chang Gung University, Taoyuan, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan; Corresponding author. Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, 123, Ta-Pei Rd., Naio-Sung, Kaohsiung 833, Taiwan.The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and is considered to be the gold standard. In this report, we share our experience of using two different testing platforms: the cobas 6800 SARS-CoV-2 test, an automated system that was recently granted Emergency Use Authorization by the FDA, and a laboratory-developed test based on the protocol from the Taiwan Centers for Disease Control (CDC). There was an overall 96.2% agreement between the two platforms. However, the positive agreement between the two platforms was only 80.0%. We found 3 instances of discordance between the two systems and this emphasized the need for timely diagnosis with a reliable testing platform.http://www.sciencedirect.com/science/article/pii/S2319417020302353CoronavirusMolecular diagnosticsViral loadRT-PCRRNA extraction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huey-Ling You Meng-Chih Lin Chen-Hsiang Lee |
spellingShingle |
Huey-Ling You Meng-Chih Lin Chen-Hsiang Lee Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19 Biomedical Journal Coronavirus Molecular diagnostics Viral load RT-PCR RNA extraction |
author_facet |
Huey-Ling You Meng-Chih Lin Chen-Hsiang Lee |
author_sort |
Huey-Ling You |
title |
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19 |
title_short |
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19 |
title_full |
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19 |
title_fullStr |
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19 |
title_full_unstemmed |
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19 |
title_sort |
comparison of the roche cobas 6800 sars-cov-2 test and the taiwan cdc protocol for the molecular diagnosis of covid-19 |
publisher |
Elsevier |
series |
Biomedical Journal |
issn |
2319-4170 |
publishDate |
2021-03-01 |
description |
The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and is considered to be the gold standard. In this report, we share our experience of using two different testing platforms: the cobas 6800 SARS-CoV-2 test, an automated system that was recently granted Emergency Use Authorization by the FDA, and a laboratory-developed test based on the protocol from the Taiwan Centers for Disease Control (CDC). There was an overall 96.2% agreement between the two platforms. However, the positive agreement between the two platforms was only 80.0%. We found 3 instances of discordance between the two systems and this emphasized the need for timely diagnosis with a reliable testing platform. |
topic |
Coronavirus Molecular diagnostics Viral load RT-PCR RNA extraction |
url |
http://www.sciencedirect.com/science/article/pii/S2319417020302353 |
work_keys_str_mv |
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